CD25 antibody | MEM-181
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|Mouse anti Human CD25, clone MEM-181 recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Norman et al. 1991) located within its extracellular domain.
The IL-2 receptor exists in three forms. A high affinity form consisting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.
CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attia et al. 2010).
Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwara et al.2013).
- Target Species
- Product Form
- Purified IgG conjugated to StarBright Violet 475 - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
- Human PHA blasts; day 3 of culture.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 475 405 479
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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References for CD25 antibody
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71.
Thorborn, G. et al. (2010) Increased sensitivity of CD4+ T-effector cells to CD4+CD25+ Treg suppression compensates for reduced Treg number in asymptomatic HIV-1 infection.
PLoS One. 5: e9254.
Cutler, A.J. et al. (2010) Umbilical cord-derived mesenchymal stromal cells modulate monocyte function to suppress T cell proliferation.
J Immunol. 185: 6617-23.
Lawson, J.M. et al. (2008) Increased resistance to CD4+CD25hi regulatory T cell-mediated suppression in patients with type 1 diabetes.
Clin Exp Immunol. 154: 353-9.
Holderness, J. et al. (2007) Select plant tannins induce IL-2Ralpha up-regulation and augment cell division in gammadelta T cells.
J Immunol. 179: 6468-78.
Zhang, Y. et al. (2013) Accelerated in vivo proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
PLoS Pathog. 9 (4): e1003310.
Nocentini, G. et al. (2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.
Arthritis Res Ther. 16: 444.
Soukup, K. et al. (2015) The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis.
J Immunol. 195 (2): 541-52.
View The Latest Product References
Kusunoki, Y. et al. (2010) T-cell immunosenescence and inflammatory response in atomic bomb survivors.
Radiat Res. 174 (6): 870-6.
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088.
Knutson, K.L. et al. (2015) Regulatory T cells, inherited variation, and clinical outcome in epithelial ovarian cancer.
Cancer Immunol Immunother. 64 (12): 1495-504.
Boland, J.W. et al. (2014) A preliminary evaluation of the effects of opioids on innate and adaptive human in vitro immune function.
BMJ Support Palliat Care. 4 (4): 357-67.
Luger, R. et al. (2013) Toll-like receptor 4 engagement drives differentiation of human and murine dendritic cells from a pro- into an anti-inflammatory mode.
PLoS One. 8 (2): e54879.
Rezalotfi, A. et al. (2020) Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells.
J Immunol Res. 2020: 6261814.
Thymianou, S et al. (2019) MBP7285 on Human Tcell Activation
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