CD20 antibody | RM272

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Rabbit anti CD20

Product Type
Monoclonal Antibody
Clone
RM272
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6340
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti CD20 antibody recognizes B-lymphocyte antigen CD20, also known as Bp35 and MS4A1.

CD20 is a non-glycosylated protein expressed on the surface of B lymphocytes and consisting of four hydrophobic transmembrane domains (Pavlasova and Mraz 2020). CD20 is involved with the regulation of transmembrane Ca2+ conductance, and is also involved with cell cycle progression during human B cell proliferation and activation (Payandeh et al. 2018). Deficiencies of CD20 have been shown to produce attenuated antibody production and germinal centre formation. Owing to its structure and expression pattern, CD20 is an important therapeutic target. Therapeutic antibodies against CD20 have revolutionized treatment of B cell haematological malignancies. Anti-CD20 antibodies also play roles in the treatment of inflammatory and autoimmune diseases (Casan et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of human CD20

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P11836
Entrez Gene
MS4A1
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0042113 B cell activation
Regulatory
For research purposes only

Applications of CD20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Fluorescent Spectraviewer

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