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CD20 antibody | 2H7

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Anti Human CD20 Antibody, clone 2H7 thumbnail image 1 Anti Human CD20 Antibody, clone 2H7 thumbnail image 2 Anti Human CD20 Antibody, clone 2H7 thumbnail image 3 Anti Human CD20 Antibody, clone 2H7 thumbnail image 4 Anti Human CD20 Antibody, clone 2H7 thumbnail image 5 Anti Human CD20 Antibody, clone 2H7 thumbnail image 6 Anti Human CD20 Antibody, clone 2H7 thumbnail image 7 Anti Human CD20 Antibody, clone 2H7 thumbnail image 8 Anti Human CD20 Antibody, clone 2H7 thumbnail image 9 Anti Human CD20 Antibody, clone 2H7 thumbnail image 10 Anti Human CD20 Antibody, clone 2H7 thumbnail image 11 Anti Human CD20 Antibody, clone 2H7 thumbnail image 12 Anti Human CD20 Antibody, clone 2H7 thumbnail image 13 Anti Human CD20 Antibody, clone 2H7 thumbnail image 14 Anti Human CD20 Antibody, clone 2H7 thumbnail image 15 Anti Human CD20 Antibody, clone 2H7 thumbnail image 16 Anti Human CD20 Antibody, clone 2H7 thumbnail image 17 Anti Human CD20 Antibody, clone 2H7 thumbnail image 18 Anti Human CD20 Antibody, clone 2H7 thumbnail image 19 Anti Human CD20 Antibody, clone 2H7 thumbnail image 20 Anti Human CD20 Antibody, clone 2H7 thumbnail image 21 Anti Human CD20 Antibody, clone 2H7 thumbnail image 22 Anti Human CD20 Antibody, clone 2H7 thumbnail image 23 Anti Human CD20 Antibody, clone 2H7 thumbnail image 24 Anti Human CD20 Antibody, clone 2H7 thumbnail image 25 Anti Human CD20 Antibody, clone 2H7 thumbnail image 26 Anti Human CD20 Antibody, clone 2H7 thumbnail image 27 Anti Human CD20 Antibody, clone 2H7 thumbnail image 28 Anti Human CD20 Antibody, clone 2H7 thumbnail image 29 Anti Human CD20 Antibody, clone 2H7 thumbnail image 30 Anti Human CD20 Antibody, clone 2H7 thumbnail image 31 Anti Human CD20 Antibody, clone 2H7 thumbnail image 32 Anti Human CD20 Antibody, clone 2H7 thumbnail image 33 Anti Human CD20 Antibody, clone 2H7 thumbnail image 34 Anti Human CD20 Antibody, clone 2H7 thumbnail image 35 Anti Human CD20 Antibody, clone 2H7 thumbnail image 36 Anti Human CD20 Antibody, clone 2H7 thumbnail image 37 Anti Human CD20 Antibody, clone 2H7 thumbnail image 38 Anti Human CD20 Antibody, clone 2H7 thumbnail image 39 Anti Human CD20 Antibody, clone 2H7 thumbnail image 40 Anti Human CD20 Antibody, clone 2H7 thumbnail image 41 Anti Human CD20 Antibody, clone 2H7 thumbnail image 42 Anti Human CD20 Antibody, clone 2H7 thumbnail image 43 Anti Human CD20 Antibody, clone 2H7 thumbnail image 44 Anti Human CD20 Antibody, clone 2H7 thumbnail image 45 Anti Human CD20 Antibody, clone 2H7 thumbnail image 46 Anti Human CD20 Antibody, clone 2H7 thumbnail image 47 Anti Human CD20 Antibody, clone 2H7 thumbnail image 48 Anti Human CD20 Antibody, clone 2H7 thumbnail image 49 Anti Human CD20 Antibody, clone 2H7 thumbnail image 50 Anti Human CD20 Antibody, clone 2H7 thumbnail image 51 Anti Human CD20 Antibody, clone 2H7 thumbnail image 52 Anti Human CD20 Antibody, clone 2H7 thumbnail image 53 Anti Human CD20 Antibody, clone 2H7 thumbnail image 54 Anti Human CD20 Antibody, clone 2H7 thumbnail image 55 Anti Human CD20 Antibody, clone 2H7 thumbnail image 56 Anti Human CD20 Antibody, clone 2H7 thumbnail image 57 Anti Human CD20 Antibody, clone 2H7 thumbnail image 58 Anti Human CD20 Antibody, clone 2H7 thumbnail image 59
Anti Human CD20 Antibody, clone 2H7 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and Amethyst Orange conjugated Mouse IgG2b isotype control (MCA691AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and Amethyst Orange conjugated Mouse anti Human CD20 (MCA1710AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD20 Antibody, clone 2H7 gallery image 2
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse anti Human CD20 (MCA1710F). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD20 Antibody, clone 2H7 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD19 (MCA2495F) and Alexa Fluor 647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. FITC conjugated Mouse anti Human CD19 (MCA2495F) and Alexa Fluor 647 conjugated Mouse anti Human CD20 (MCA1710A647). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD20 Antibody, clone 2H7 gallery image 4
Published customer image:
APC conjugated Mouse anti Human CD20 antibody, clone 2H7 (MCA1710APC) used as part of a panel of antibodies to identify lymphocyte populations in feline peripheral blood by flow cytometry.
Image caption:
Gating strategy used in flow cytometric analyses of PBMC.
Representative pseudocolor plots are shown. After exclusion of dead cells (A) and doublets (B), gating on lymphocytes and monocytes was performed according to their forward and side scattering (FSC/SSC)properties (C). (D) Among lymphocytes CD4+ T cells, CD8+ T cells and CD20+ B cells (upper panel) were gated according to the appropriate Fluorescence-minus-one (FMO) controls (lower panel).

From: Sieg M, Busch J, Eschke M, Böttcher D, Heenemann K, Vahlenkamp A, Reinert A, Seeger J, Heilmann R, Scheffler K, Vahlenkamp TW.
A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.
Viruses. 2019 Feb 9;11(2). pii: E146.
doi: 10.3390/v11020146.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD20 Antibody, clone 2H7 gallery image 5
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488) and SBV515 conjugated Mouse anti Human CD19 (MCA1940SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 6
Figure A. PE conjugated Mouse anti Human CD19 (MCA1940PE). Figure B. PE conjugated Mouse anti Human CD19 (MCA1940PE) and SBV515 conjugated Mouse anti Human CD20 (MCA1710SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 7
Mouse anti Human CD20 antibody, clone 2C7 (MCA1710) in an immunohistochemical analysis of acetone fixed, frozen human tonsil section.
Anti Human CD20 Antibody, clone 2H7 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488) and StarBright Violet 670 conjugated Mouse anti Human CD19 (MCA1940SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 9
Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 10
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD19 (MCA1940SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD19 (MCA1940SBV790) and StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 11
Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 12
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright Violet 670 conjugated Mouse anti Human CD20 (MCA1710SBV670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 13
Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 570 conjugated Mouse anti Human CD19 (MCA1940SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 14
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710) and StarBright Violet 570 conjugated Mouse anti Human CD20 (MCA1710SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 15
Figure A. PE conjugated Mouse anti Human CD20 (MCA1710PE). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 710 conjugated Mouse anti Human CD19 (MCA1940SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 16
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515) and StarBright Violet 790 conjugated Mouse anti Human CD19 (MCA1940SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 17
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD19 (MCA1940SBV515) and StarBright Violet 790 conjugated Mouse anti Human CD20 (MCA1710SBV790). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 18
Figure A. PE conjugated Mouse anti Human CD20 ( ). Figure B. PE conjugated Mouse anti Human CD20 (MCA1710PE) and StarBright Violet 475 conjugated Mouse anti Human CD19 (MCA1940SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 19
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1940SBV710). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1940SBV710) and StarBright Violet 475 conjugated Mouse anti Human CD20 (MCA1710SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 20
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 21
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD20 (MCA1710SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD20 (MCA1710SBV670) and StarBright UltraViolet 400 conjugated Mouse anti Human CD19 MCA1940SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 22
Figure A. StarBright UltraViolet 510 conjugated Mouse anti Human CD20 (MCA1710SBUV510). Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD20 (MCA1710SBUV510) and StarBright Violet 760 conjugated Mouse anti Human CD19 (MCA1940SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 23
Figure A. Starbright UltraViolet conjugated Mouse anti Human CD19 (MCA1940SBUV510). Figure B. Starbright UltraViolet conjugated Mouse anti Human CD19 (MCA1940SBUV510) and StarBright Violet 760 conjugated Mouse anti Human CD20 (MCA1710SBV760). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 24
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD20 (MCA1710A488) and StarBright UltraViolet 665 conjugated Mouse anti Human CD19 (MCA1940SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 25
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400) and StarBright UltraViolet 665 conjugated Mouse anti Human CD20 (MCA1710SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 26
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD19 (MCA1940A647) and StarBright UltraViolet 445 conjugated Mouse anti human CD20 (MCA1710SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 27
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD20 (MCA1710SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD20 (MCA1710SBB700) and StarBright UltraViolet 445 conjugated Mouse anti Human CD19 (MCA1940SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 28
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440) and StarBright UltraViolet 795 conjugated Rat anti Mouse CD19 (MCA1940SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 29
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD19 (MCA1940A488) and StarBright UltraViolet 795 conjugated Mouse anti human CD20 (MCA1710SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 30
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440) and StarBright UltraViolet 510 conjugated Rat anti Mouse CD19 (MCA1940SBUV510). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 31
Figure A. StarBright Violet 670 conjugated Mouse anti Human CD19 (MCA1940SBV670). Figure B. StarBright Violet 670 conjugated Mouse anti Human CD19 (MCA1940SBV670) and StarBright UltraViolet 510 conjugated Mouse anti Human CD20 (MCA1710SBUV510). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 32
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515) and StarBright UltraViolet 575 conjugated Mouse anti Human CD19 (MCA1940SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 33
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD19 (MCA1940A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD19 (MCA1940A700) and StarBright UltraViolet 605 conjugated Mouse anti Human CD20 (MCA1710SBUV605). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 34
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400) and StarBright UltraViolet 605 conjugated Mouse anti Human CD19 (MCA1940SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 35
Figure A. PE-Alexa Fluor 750 conjugated Mouse anti Human CD19 (MCA1940P750). Figure B. PE-Alexa Fluor 750 conjugated Mouse anti Human CD19 (MCA1940P750) and StarBright Blue 580 conjugated Mouse anti Human CD20 (MC1710SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 36
Figure A. StarBright Ultra Violet 510 conjugated Mouse anti Human CD20 (MCA1710SBUV510). Figure B. StarBright Ultra Violet 510 conjugated Mouse anti Human CD20 (MCA1710SBUV510) and StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 37
Figure A. RPE-Alexa Fluor 750 conjugated Mouse anti Human CD19 (MCA1940P750). Figure B. RPE-Alexa Fluor 750 conjugated Mouse anti Human CD19 (MCA1940P750) and StarBright Blue 615 conjugated Mouse anti Human CD20 (MCA1710SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 38
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440) and StarBright Blue 615 conjugated Mouse anti Human CD19 (MCA1940SBB615). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 39
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD20 (MCA1710SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 40
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440) and StarBright Blue 675 conjugated Mouse anti Human CD19 (MCA1940SBB675). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 41
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610) and StarBright Blue 765 conjugated Mouse anti Human CD20 (MCA1710SBB765). All experiments performed on red blood cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 42
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD20 (MCA1710SBV440) and StarBright Blue 765 conjugated Mouse anti Human CD19 (MCA1940SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 43
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD19 (MCA1940SBV440) and StarBright Blue 810 conjugated Mouse anti Human CD20 (MCA1710SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 44
Figure A. StarBright Ultra Violet 605 conjugated Mouse anti Human CD20 (MCA1710SBUV605). Figure B. StarBright Ultra Violet 605 conjugated Mouse anti Human CD20 (MCA1710SBUV605) and StarBright Blue 810 conjugated Mouse anti Human CD19 (MCA1940SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 45
Figure A. StarBright UltraViolet 795 conjugated Mouse anti Human CD20 (MCA1710SBUV795). Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD20 (MCA1710SBUV795) and StarBright Yellow 575 conjugated Mouse anti Human CD19 (MCA1940SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD20 Antibody, clone 2H7 gallery image 46
Figure A. StarBright Blue 765 conjugated Mouse anti Human CD19 (MCA1940SBB765). Figure B StarBright Blue 765 conjugated Mouse anti Human CD19 (MCA1940SBB765) and StarBright Yellow 575 conjugated Mouse anti Human CD20 (MCA1710SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 47
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400) and StarBright Yellow 720 conjugated Mouse anti Human CD19 (MCA1940SBY720). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 48
Figure A. StarBright Yellow 575 conjugated Mouse anti Human CD20 (MCA1940SBY575). Figure B. StarBright Yellow 575 conjugated Mouse anti Human CD20 (MCA1940SBY575) and StarBright Yellow 720 conjugated Mouse anti Human CD20 (MCA1710SBY720). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 49
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD20 (MCA1710SBV515) and StarBright Yellow 605 conjugated Mouse anti Human CD19 (MCA1940SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 50
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD19 (MCA1940SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD19 (MCA1940SBV475) and StarBright Yellow 605 conjugated Mouse anti Human CD20 (MCA1710SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 51
Figure A. StarBright Ultra Violet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). Figure B. StarBright Ultra Violet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400) and StarBright Yellow 665 conjugated Mouse anti Human CD20 (MCA1710SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 52
Figure A. StarBright Violet 570 conjugated Mouse anti Human CD20 (MCA1710SBV570). Figure B. StarBright Violet 570 conjugated Mouse anti Human CD20 (MCA1710SBV570) and StarBright Yellow 800 conjugated Mouse anti Human CD19 (MCA1940SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 53
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD20 (MCA1710SBUV400) and StarBright Yellow 665 conjugated Mouse anti Human CD19 (MCA1940SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 54
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD19 (MCA1940SBUV400) and StarBright Yellow 800 conjugated Mouse anti Human CD20 (MCA1710SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 55
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD19 (MCA1940SBV610) and StarBright Red 775 conjugated Mouse anti Human CD20 (MCA1710SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 56
Figure A. StarBright Blue 675 conjugated Mouse anti Human CD20 (MCA1710SBB675). Figure B. StarBright Blue 675 conjugated Mouse anti Human CD20 (MCA1710SBB675) and StarBright Red 815 conjugated Mouse anti Human CD19 (MCA1940SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 57
Figure A. StarBright Blue 675 conjugated Mouse anti Human CD19 (MCA1940SBB675). Figure B. StarBright Blue 675 conjugated Mouse anti Human CD19 (MCA1940SBB675) and StarBright Red 815 conjugated Mouse anti Human CD20 (MCA1710SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 58
Figure A. StarBright Yellow 605 conjugated Mouse anti Human CD20 (MCA1710SBY605). Figure B. StarBright Yellow 605 conjugated Mouse anti Human CD20 (MCA1710SBY605) and StarBright UltraViolet 740 conjugated Mouse anti Human CD19 (MCA1940SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD20 Antibody, clone 2H7 gallery image 59
Figure A. StarBright Yellow 605 conjugated Mouse anti Human CD19 (MCA1940SBY605). Figure B. StarBright Yellow 605 conjugated Mouse anti Human CD19 (MCA1940SBY605) and StarBright UltraViolet 740 conjugated Mouse anti Human CD20 (MCA1710SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD20:StarBright Yellow 720

Product Type
Monoclonal Antibody
Clone
2H7
Isotype
IgG2b
Specificity
CD20

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Product Code Applications Pack Size List Price Your Price Qty
MCA1710SBY720
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SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
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Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.

The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.

The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Yellow 720 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Yellow 720 549 719
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for CD20 antibody

  1. Chan, H.T. et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts.
    Cancer Res. 63: 5480-9.
  2. Cragg, M.S. et al. (2003) Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts.
    Blood. 101: 1045-52.
  3. Jaramillo, M.C. et al. (2009) Increased manganese superoxide dismutase expression or treatment with manganese porphyrin potentiates dexamethasone-induced apoptosis in lymphoma cells.
    Cancer Res. 69: 5450-7.
  4. Teeling, J.L. et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20.
    J Immunol. 177 (1): 362-71.
  5. Polyak, M.J. & Deans, J.P. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
    Blood. 99 (9): 3256-62.
  6. Greig, B. et al. (2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.
    Cytometry B Clin Cytom. 86: 135-8.
  7. van den Akker, E. et al. (2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.
    Haematologica. 95: 1594-8.
  8. Jaramillo, M.C. et al. (2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.
    Free Radic Biol Med. 83: 89-100.
    9. View The Latest Product References
  9. Cecchinato, V. et al. (2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.
    J Immunol. 198 (1): 184-195.
  10. Kohler, S.L. et al. (2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.
    J Immunol. 196 (6): 2711-22.
  11. Grobárová V et al. (2016) Quambalarine B, a Secondary Metabolite from Quambalaria cyanescens with Potential Anticancer Properties.
    J Nat Prod. 79 (9): 2304-14.
  12. Popov, J. et al. (2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.
    Eur J Pharm Biopharm. 117: 256-69.
  13. Sieg, M. et al. (2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.
    Viruses. 11 (2): 146.

Flow Cytometry

UniProt
P11836
Entrez Gene
MS4A1
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0042113 B cell activation

MCA1710SBY720

100007334

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