CD20 antibody | 2H7
Mouse anti Human CD20:StarBright Violet 570
- Product Type
- Monoclonal Antibody
- Clone
- 2H7
- Isotype
- IgG2b
- Specificity
- CD20
Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells. The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 570 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 570 404 571 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD20 antibody
-
Chan, H.T. et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts.
Cancer Res. 63: 5480-9. -
Cragg, M.S. et al. (2003) Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts.
Blood. 101: 1045-52. -
Jaramillo, M.C. et al. (2009) Increased manganese superoxide dismutase expression or treatment with manganese porphyrin potentiates dexamethasone-induced apoptosis in lymphoma cells.
Cancer Res. 69: 5450-7. -
Teeling, J.L. et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20.
J Immunol. 177 (1): 362-71. -
Polyak, M.J. & Deans, J.P. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Blood. 99 (9): 3256-62. -
Greig, B. et al. (2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.
Cytometry B Clin Cytom. 86: 135-8. -
van den Akker, E. et al. (2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.
Haematologica. 95: 1594-8. -
Jaramillo, M.C. et al. (2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.
Free Radic Biol Med. 83: 89-100.
View The Latest Product References
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Cecchinato, V. et al. (2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.
J Immunol. 198 (1): 184-195. -
Kohler, S.L. et al. (2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.
J Immunol. 196 (6): 2711-22. -
Grobárová V et al. (2016) Quambalarine B, a Secondary Metabolite from Quambalaria cyanescens with Potential Anticancer Properties.
J Nat Prod. 79 (9): 2304-14. -
Popov, J. et al. (2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.
Eur J Pharm Biopharm. 117: 256-69. -
Sieg, M. et al. (2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.
Viruses. 11 (2): 146.
- UniProt
- P11836
- Entrez Gene
- MS4A1
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0042113 B cell activation
MCA1710SBV570
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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