CD20 antibody | 2H7

Mouse anti Human CD20:StarBright Violet 570

Product Type
Monoclonal Antibody
Clone
2H7
Isotype
IgG2b
Specificity
CD20

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MCA1710SBV570
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Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.

The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells.

The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 570 402 570
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD20 antibody

  1. Chan, H.T. et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts.
    Cancer Res. 63: 5480-9.
  2. Cragg, M.S. et al. (2003) Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts.
    Blood. 101: 1045-52.
  3. Jaramillo, M.C. et al. (2009) Increased manganese superoxide dismutase expression or treatment with manganese porphyrin potentiates dexamethasone-induced apoptosis in lymphoma cells.
    Cancer Res. 69: 5450-7.
  4. Teeling, J.L. et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20.
    J Immunol. 177 (1): 362-71.
  5. Polyak, M.J. et al. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
    Blood. 1;99:3256-62.
  6. Greig, B. et al. (2014) Stabilization media increases recovery in paucicellular cerebrospinal fluid specimens submitted for flow cytometry testing.
    Cytometry B Clin Cytom. 86: 135-8.
  7. van den Akker, E. et al. (2010) The majority of the in vitro erythroid expansion potential resides in CD34(-) cells, outweighing the contribution of CD34(+) cells and significantly increasing the erythroblast yield from peripheral blood samples.
    Haematologica. 95: 1594-8.
  8. Jaramillo, M.C. et al. (2015) Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.
    Free Radic Biol Med. 83: 89-100.
  9. View The Latest Product References
  10. Cecchinato, V. et al. (2017) Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.
    J Immunol. 198 (1): 184-195.
  11. Kohler, S.L. et al. (2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication.
    J Immunol. 196 (6): 2711-22.
  12. Grobárová V et al. (2016) Quambalarine B, a Secondary Metabolite from Quambalaria cyanescens with Potential Anticancer Properties.
    J Nat Prod. 79 (9): 2304-14.
  13. Popov, J. et al. (2017) Unique therapeutic properties and preparation methodology of multivalent rituximab-lipid nanoparticles.
    Eur J Pharm Biopharm. 117: 256-69.
  14. Sieg, M. et al. (2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.
    Viruses. 11 (2) Feb 09 [Epub ahead of print].

Flow Cytometry

UniProt
P11836
Entrez Gene
MS4A1
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0042113 B cell activation
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