CD163 antibody | EDHu-1
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification of infiltrating macrophages in the pulmonary tissues of cattle by immunohistochemistry on formalin fixed tissue sections.
Image caption:
Immunohistochemical Labeling of Leukocytes in the Lungs.
Shown are the results of IHC labeling for CD3-like immunoreactivity (CD3-li), CD163-li, IBA-1-li, and IL-17-li in the lung of uninfected control and representative infected Holstein calves. Note: CD3-li, CD163-li, and IL-17-li is significantly increased in the lungs of the infected calf. Furthermore, CD3+, CD163+, IBA-1+ and IL-17+ mononuclear cells are components of vasculitis lesions. Vascular endothelial cells consistently exhibit pronounced IL-17-li in the infected calf, but endothelial IL-17-li is rare in the control calf. Scale bar: 200 μm.
From: Fry LM, Schneider DA, Frevert CW, Nelson DD, Morrison WI, Knowles DP (2016)
East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
PLoS ONE 11(5): e0156004.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.
Image caption:
Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D. Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i). The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n). Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 μm, (b), (c) to (v) 5 μm.
From: Schultz, N. et al. (2016).
Amylin alters human brain pericyte viability and NG2 expression.
J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of CD163 expression on porcine alveolar macrophages by flow cytometry.
Image caption:
Morphologic and phenotypic characterization of porcine alveolar macrophage cells (PAM) by flow cytometry using SWC3, SWC1, CD163, CD14, CD16, MHCII and DC-sign staining. This figure is a representative of three independent experiments
From: Seeboth J, Solinhac R, Oswald IP, Guzylack-Piriou L.
The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 2012 Apr 24;43:35.
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of perivascular macrophages on guinea pig myocardial tissue sections by immunofluorescence on formalin fixed, paraffin embedded tissue sections following heat mediated antigen retrieval in sodium citrate buffer pH 6.0.
Image caption:
Immunofluorescence analysis of myocardial HO-1 and CD163 expression. Surgical non-treated (NT) control animals showed minimal HO-1 (green) expression in resident CD163+ perivascular macrophages (red) (A–C). Sporadic HO-1 expression (white arrow) localized to CD163+ cells observed with Hb alone (D–F). With LPS alone, CD163+ infiltrates and perivascular macrophages showed minimal HO-1 expression (white arrowhead) (G–I). Increased HO-1 expression localized to CD163+ infiltrates and perivascular macrophages observed with LPS + Hb (white arrows) (J–L,M–O). Hp reduces HO-1 expression in CD163+ infiltrates and perivascular cells (P–R,S–U). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 10 μm.
From: Baek JH, Zhang X, Williams MC, Schaer DJ, Buehler PW, D'Agnillo F.
Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel). 2014 Mar 31;6(4):1244-59.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of macrophage infiltration in the brains of SIV infected Rhesus macaques by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Massive activation and productive infection of microglia in CD4-depleted RMs. (a) ISH showing the levels of SIV vRNA+ cells in brain from a representative SIV-uninfected control (top), SIV-infected control (middle) and CD4-depleted SIV-infected (bottom) animal. The amount of SIV vRNA+ cells was markedly higher in CD4-depleted animals than in controls. (b) Immunofluorescence staining for CD163 (left panels), HLA-DR (middle panels) and proliferating cell nuclear antigen (PCNA; right panels) in the parenchyma of one representative SIV-uninfected control (top), one SIV-infected control (middle) and one SIV-infected CD4-depleted (bottom) RM. Nuclei are stained in green; markers of interest in red. (c) Quantitative analyses showing the number of cells per mm2 of tissue that stained positively for the markers of interest. Increased expression of CD163, HLA-DR and PCNA was found in CD4-depleted animals (orange square; n = 4) when compared to both groups of controls (SIV- open circle, n = 3; SIV+ closed circle, n = 3). (d) Single and combined staining for IBA-1 (green), CD163 (blue), and SIV-vRNA (red) in the brain of one representative SIV-infected CD4 depleted RM. The box on the right is a magnification of a microglial cell (IBA-1+) that expresses CD163 and is productively infected (SIV vRNA+).
From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014)
CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells.
PLoS Pathog 10(10): e1004467.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification of intestinal and lymph node macrophages in SIV infected macaques by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Massive SIV infection of LN and intestinal macrophages in CD4-depleted RMs. (a) Immunofluorescence staining for T cell (CD3; blue) and macrophage (CD68 and/or CD163; green) lineage markers combined with in situ hybridization for SIV vRNA (red) in the LN isolated at day 42 post-infection from one representative undepleted control (left) and one CD4-depleted RM. The vast majority of SIV vRNA+ cells express CD3 in undepleted control but express CD68/CD163 in CD4-depleted RM. (b) The same staining is shown for colon (top) and jejunum (bottom) tissues in two representative CD4-depleted animals. (c) Quantitative image analysis of LN and mucosal tissues showing the fraction of SIV vRNA+ cells that express CD3 or CD68/CD163 in CD4-depleted (n = 12; the 8 animals in this study plus the 4 in Ortiz et al 2011) or undepleted control RMs (n = 6; two SIVmac251 infected animals belonging to a different study were added as controls). (d) Relative abundance of SIV vRNA+ in productively infected T cells and macrophages, determined by measuring the volumetric sum of the SIV vRNA+ intensity integration values in situ from confocal images collected under identical laser settings, is shown in four CD4-depleted RMs. Macrophages on average have higher per cell SIV vRNA+ content compared to CD4+ T cells within the same host. Statistical analyses were determined by Mann-Whitney test.
From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014)
CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells.
PLoS Pathog 10(10): e1004467.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in vitro by immunofluorescence.
Image caption:
Expression of Mf-markers and NF-κB subunits in co-cultures of HSC-3 cells and M1 or R848 Mfs. Vybrant CM-Dil labeled HSC-3 cells (red) and unlabeled M1- or R848 Mfs were incubated with DMSO or 10 ng/ml TNF-α for 30 min where after cells were fixed for immunofluorescence with antibodies for CD68, CD163, NF-κB p50 and p65 subunits. Some samples were pre-incubated with 10 μM BAY 11-7082 prior to TNF-α activation. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mountain medium to visualize nuclei (blue). Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD163 and NF-κB p65 immunofluorescence from HSC-3 cell and M1 Mf co-cultures (A). CD68 and NF-κB p50 immunofluorescence from HSC-3 cell and R848 Mf co-cultures (B). Red arrows indicate HSC-3 cells and green arrows Mfs. Scale bars 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in human tongue carcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
TAMs localize to evading carcinoma cells in vivo. Human tongue carcinoma tissue sections were subjected to double-staining immunohistochemistry with antibodies for CD163, NF-κB p50 and NF-κB p65. CD163 (red) and NF-κB p50 (grey) staining in A, B. Black arrows show cells with both red and grey color, indicating co-localization of the antibodies. CD163 (red) and NF-κB p65 (grey) staining in C,D, G,H. Blue arrows in H show tongue carcinoma cells. NF-κB p50 (grey) and NF-κB p65 (red) staining in E,F. Black boxes in A, C and G indicates site of magnification in B, D and H respectively. Scale bars in A,C,E and G 200 μm and in B, D, F, and H 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in vitro by immunofluorescence.
Image caption:
Vybrant CM-Dil labeled HSC-3 cells (red) and unlabeled M1-, M2 and R848 Mfs were incubated with DMSO where after cells were fixed for immunofluorescence with antibodies for CD68, CD163 and pancytokeratin. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mounting medium to visualize nuclei (blue). Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD68 marker staining in DMSO-treated M1 Mfs (A), M2 Mfs (B) and R848 Mfs (C). CD163 marker staining in DMSO-treated M2 Mfs treated (D) and R848 Mfs (E). DMSO-treated HSC-3 cells (red) stained with pancytokeratin (green) in F. Scale bars 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification and enumeration of infiltrating macrophages in both polyploid and non-polyploid colorectal adenoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical assessment of stromal immune cell infiltrates in polypoid and nonpolypoid colorectal adenomas.
CD4+, FOXP3+, CD8+, CD68+, CD163+, and MHC-I+ immune cell densities (i.e., number of positively-stained cells per unit area; see Methods) in the stroma of polypoid adenomas were consistently higher than those observed in nonpolypoid lesions. Intraepithelial CD8+ (cytotoxic) T cells are also seen in the epithelial compartments of both types of tumor. In calculating CD68+ and CD163+ macrophage densities, we counted only nuclei surrounded by CD68+ or CD163+ granulations and excluded elongated or fragmented cells with granular cytoplasmic positivity. MHC-I labelling (granular, compact, or membranous) was observed in a variety of stromal cells. MHC-I+ epithelial cells were also present in both types of tumor, but their presence was not quantified in this study. Magnification for all panels: 400X.
From: Maglietta A, Maglietta R, Staiano T, Bertoni R, Ancona N, Marra G, et al. (2016)
The Immune Landscapes of Polypoid and Nonpolypoid Precancerous Colorectal Lesions.
PLoS ONE 11(7): e0159373.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody clone EDHu-1 (MCA1853) used for the identification of skin macrophages by immunohistochemistry.
Image caption:
EBV-lytic proteins are expressed in dcSSc circulating monocytes and dcSSc skin macrophages. Immunohistochemistry of skin sections, representative immunohistologic images of CD163+ macrophages (brown) and EBV/lytic protein Zebra + (blue) cells in skin sections from dcSSc and HD. Original magnification 2× (upper panels) and 10× (inserts and lower panels). The arrows indicate monocytes represented in the high magnification inserts. Scale bars = 5μm
From: Farina A, Peruzzi G, Lacconi V, Lenna S, Quarta S, Rosato E, Vestri AR, York M, Dreyfus DH, Faggioni A, Morrone S, Trojanowska M, Farina GA.
Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
Arthritis Res Ther. 2017 Feb 28;19(1):39.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the demonstration of CD163 expression on liver macrophages from patients with primary sclerosing cholangitis by immunohistochemistry on formalin fixed OCT-embedded cryosections.
Image caption:
T cells infiltrate PSC-patient livers. Representative immunostaining and quantification analysis of (A) CD3, (B) TCR-Vα7.2, (C) NKp46, (D) CD163, and (E) MHC class I expression in controls (n = 14–17) and PSC patients (n = 14–17). Samples were counterstained with hematoxylin and dark brown indicate immunoreaction product. Quantification was performed to calculate mean intensity of the stainings (ACIA) and frequency of expression (percentage of stained area out of total area).
From: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, et al. (2014)
In Situ Characterization of Intrahepatic Non-Parenchymal Cells in PSC Reveals Phenotypic Patterns Associated with Disease Severity.
PLoS ONE 9(8): e105375.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label macrophages in human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunostaining of CD163 and CD68 on consecutive sections of paired tumor tissue and peritumoral liver tissue.
The densities of intratumoral CD68+ cells (A) and CD163+ cells (C) were lower in tumor than in corresponding peritumoral liver tissue (B and D). The density of CD163+ cells was higher than that of CD68+ cells in both tumor and in peritumoral liver tissue. Scale bar, 200 μm.
From: Kong L-Q, Zhu X-D, Xu H-X, Zhang J-B, Lu L, Wang W-Q, et al. (2013)
The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma.
PLoS ONE 8(3): e59771. doi: 10.1371/journal.pone.0059771
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to visualize M2 macrophages in human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of CD163 in liver sections, serum concentration of sCD163 and proportion of CD14 in controls and patients with CHC with different severities of fibrosis (F<2 or F≥2). (A-C) Representative histology images of liver sections with (A) H&E, (B) Masson-trichrome and (C) CD163 immunohistochemical staining (original magnification, x200). In CHC liver tissues, enhanced expression of CD163 was localized in the fibrous septum.
From: Zhao, S., Li, W., Fu, N., Kong, L., Zhang, Q., Han, F., Ren, W., Cui, P., Du, J., Wang, B., Zhang, Y., Wang, R., Kong, L., Nan, Y.
"CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C".
Experimental and Therapeutic Medicine 20.6 (2020): 228.
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label macrophages in Cynomolgus monkey endometrium by immunohistochemistry on formalin fixed , paraffin embedded tissue sections.
Image caption:
Histology of endometriosis from an advanced lesion and an early lesion in cynomolgus monkeys.
For an early lesion, a tissue was taken from a brown deposition on the peritoneum from the vesicouterine pouch of monkey #7 that died of acute gastrectasia unrelated to endometriosis two days after the follow-up laparoscopy performed 11 months after the initial laparoscopy (G-L).(G) Inflammation and an endometrial gland were seen in the peritoneum of the tissue (× 40). (H) Higher magnification of G shows infiltration of mononuclear cells (× 400). (I) Higher magnification of G shows an endometrial gland (× 400). (J) CD3-positive lymphocytes (T cells) seen in the same location of H in a serial section (× 400). (K) CD163-positive macrophages and macrophage-derived foreign body multinucleated giant cells (arrow) seen in the same location of H in a serial section (× 400). (L) Expression of estrogen receptor alpha of an endometrial gland seen in the same location of I in a serial section (× 400). G, H, and I (H & E staining); J, K, and L (Immunohistochemistry).
From: Hayashi K, Nakayama M, Iwatani C, Tsuchiya H, Nakamura S, Nonoguchi K, Itoh Y, Tsuji S, Ishigaki H, Mori T, Murakami T, Ogasawara K.
The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
Tohoku J Exp Med. 2020 Aug;251(4):241-53.
doi: 10.1620/tjem.251.241.
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Mouse anti Human CD163 antibody, clone EDHu-1 used for demonstrating CD163 expression by anthracosis associated myeloid cells by immunohistochemistry.
Image caption:
Tumor-promoting phenotype of the myeloid clusters. IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P<0.001. (Scale bar, 200 μm for 100× and 400×; 25 μm for 1600×)
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody (MCA1853) used for the evaluation of CD163 expression on microglia by immunohistochemistry on formalin fixed, paraffin embedded brain sections.
Image caption:
Rasmussen's encephalitis. (A) Areas of active encephalitis and cortical scarring highlighted with increased numbers of HLADR-positive microglia/macrophages, as well as interstitial rod-like CD163-positive microglia cells (inset). (B) On adjacent sections, labelling with pS6 235/236 showed a similar distribution of cellular labelling as well as with pS6 240/244 (C). (D) pS6 240/244 highlighted many of the enlarged dysmorphic neurones in RE cases, as confirmed with co-labelling with neurofilaments (inset). (E) pS6 236/26 demonstrated labelling of smaller glial cells as well as (F) bipolar rod shape cells. Double labelling studies in RE cases confirmed the majority of pS6 labelled cells were not GFAP-positive astroglia (G) but co-labelled with populations of iba1-positive cells (microglial marker) (H), nestin and doublecortin (inset) (I) positive cells. Bar in A, B, C equivalent to approximately 100 microns, in d approximately 50 microns and E, F, G, H, I approximately 30 microns.
From: Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM, Thom M.
Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2014 Jul 8;2:71.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Identification of residues in pCD163 SRCR5 important for PRRSV-2 infection. A RT-qPCR analyses of the effect of mutated pCD163 on PRRSV-2 replication. WT or mutant pCD163 constructs (3 μg/well, 6-well plate) were transfected into PK-15 cells. After 24 h, the transfected PK-15 cells were inoculated with PRRSV-2 strain BJ-4 at a MOI of 1. At 12 hpi, total RNAs of infected PK-15 cells were extracted and then the viral RNA was measured by RT-qPCR. In parallel, expression levels of WT and mutant pCD163 were tested by IB using a commercial mouse anti-human CD163 antibody (MCA1853). Data represent means ± SEM from three independent experiments. *p <0.05.
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Identification of residues in pCD163 SRCR5 important for PRRSV-2 infection.B Analyses of PRRSV N protein expression in WT or mutant pCD163-expressed cells. The infected WT or mutant pCD163-expressed cells were harvested and lysed at 24 hpi. PRRSV N protein in WT or mutant pCD163-expressed cells was measured by IB. Significant reduction in PRRSV N protein expression is marked by an arrow. In parallel, expression levels of WT and mutant pCD163 were tested by IB using a commercial mouse anti-human CD163 antibody (MCA1853).
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Analyses of simultaneous mutagenesis of the identified two residues for PRRSV-2 infection. A TCID50 analyses of the effect of mutated CD163 on PRRSV-2 viral titers. PK-15 cells were transfected with WT or mutated pCD163 constructs. After 24 h, the transfected PK-15 cells were inoculated with PRRSV strain BJ-4 or HN07-1 at a MOI of 1 for 48 h. The viral yields were measured by TCID50 assay in MARC-145 cells. Data represent means ± SEM of three independent experiments. **p <0.01, ***p <0.001 for mutant CD163 compared to the WT one. #p <0.05 for simultaneous mutagenesis at position 534 and 561 of CD163 compared to the single-site mutagenesis. B Analyses of PRRSV N protein expression in WT or mutant pCD163-expressed cells. PRRSV N protein expression was tested by IB as described in Figure 5B.
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label M2 type macrophages in high-grade glioma tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry. Representative microscopic images (200x magnification, scale bar 100 μm) of one GBM patient including the HE (first column) and the IHC staining for CD45 (second column), CD68 (third column) and CD163 (fourth column) Images demonstrate the morphological heterogeneity within the tumor tissue (A), the tumor core (B), the infiltration zone (C) and the perivascular area (D).
From: Kemmerer CL, Schittenhelm J, Dubois E, Neumann L, Häsler LM, Lambert M, Renovanz M, Kaeser SA, Tabatabai G, Ziemann U, Naumann U, Kowarik MC.
Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients.
BMC Cancer. 2021 Oct 15;21(1):1108.
doi: 10.1186/s12885-021-08825-1.
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label CD163 expressing cells in ovine tissues by immunohistochemistry of formalin fixed, paraffin embedded tissue sections following proteinase K mediated antigen retrieval.
Image caption:
Representative images of sections of the native ovine ACL and intra-articular graft portion stained with antibodies to CD3, Ki-67, MAC 387; CD163 and CTGF.
A-E Native ovine ACL; F-J decellularised pSFT explanted at 4 weeks; K–O; decellularised pSFT explanted at 12 weeks; P-T decellularised pSFT explanted at 26 weeks; U–Y ovine SFT (allograft control) explanted at 26 weeks. Images captured at 10× magnification unless otherwise stated. Scale bars show 100 μm.
From: Edwards JH, Jones GL, Herbert A, Fisher J, Ingham E.
Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
Biomaterials. 2021 Oct 21;279:121204.
doi: 10.1016/j.biomaterials.2021.121204
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Mouse anti Human macrophages, clone MAC387 (MCA874GA) used to label macrophages in ovine pulmonary root tissues by immunohistochemistry on formalin fixed cryosections.
Image caption:
Representative images of sections of explanted pulmonary root wall tissues stained with antibodies to α-SMA, vimentin, MAC 387, CD163, CD34, CD271, vWF and CTGF. (a) Upper panel: native ovine pulmonary artery wall tissues. Images captured at 10× magnification (scale bars 100 μm) unless otherwise stated. α-SMA (scan 2.5× magnification; scale bar 2000 μm), CD34 and vWF (20× magnification; scale bars 50 μm). Lower panel: decellularised porcine pulmonary wall tissues explanted after 12 months. Images captured at 10× magnification (scale bars 100 μm) unless otherwise stated. α-SMA (2.5× magnification; scale bar 500 μm), vimentin and vWF (20× magnification; scale bars 50 μm). Black arrows indicate the intima. (b) Images of the distal decellularised porcine pulmonary artery wall tissues explanted at 1 month. Images captured at 2.5× magnification (scale bars 500 μm). Images show MAC 387+ and CD163+ cells at the interface between cellular and acellular tissue with α-SMA+ and CD271+ cells populating the adventitia and media. Black arrows: intima. (c) Upper panel: images of the same region of central area of a decellularised porcine pulmonary artery wall tissue explanted at 3 months captured at 2.5× magnification (scale bars 500 μm). Images show the ‘front’ (dashed line) of MAC 387+ and CD 163+ cells between the media and intimal regions with α-SMA+ cells in the central media. Black arrows: intima. Lower panel: images of sequential sections of the central area of a decellularised porcine pulmonary artery wall tissue explanted at 3 months captured at 20× magnification (scale bars 50 μm) clearly showing distinct populations of MAC 387+/ CD163low; CD163+/ MAC 387+; CD34+ and CD271+ cells. Red circle: vessel present in all images. The dashed lines demarcate cellular and acellular tissue. (d) Ovine allograft pulmonary artery wall tissues explanted at 12 months. Images captured at 10× magnification (scale bars 100 μm) except α-SMA (2.5× magnification; scale bar 500 μm). Images show the presence of α-SMA+, CD163+ and CD34+ cells in the media with an amorphous intimal region and the presence of MAC 387+ cell foci at the intima with high levels of expression of CTGF and vWF. Black arrows: intima.
From: Vafaee T, Walker F, Thomas D, Roderjan JG, Veiga Lopes S, da Costa FD, Desai A, Rooney P, Jennings LM, Fisher J, Berry HE, Ingham E.
Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
J Tissue Eng. 2022 Jun 28;13:20417314221102680.
doi: 10.1177/20417314221102680.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human macrophages, clone MAC387 (MCA874GA) used to label macrophages in ovine pulmonary root tissues by immunohistochemistry on formalin fixed cryosections.
Image caption:
Representative images of sections of explanted pulmonary root leaflet tissues stained with antibodies to α-SMA, vimentin, MAC 387, CD163, CD34 or CTGF. (a) Upper panel: native ovine pulmonary root leaflet tissues. Images captured at 10× magnification (scale bars 100 μm) except CD34 (20× magnification; scale bar 50 μm). Lower panel: decellularised porcine pulmonary wall tissues explanted after 12 months in vivo. Images captured at 10× magnification (scale bars 100 μm) except vimentin (20× magnification; scale bar 50 μm). Images show that the distribution of cells expressing α-SMA, vimentin and CD163 was similar, although sparser, in the decellularised porcine pulmonary root leaflet tissues compared to native ovine tissue, however cells in the decellularised porcine pulmonary root leaflets did not express CD34 and there were high levels of expression of CTGF.
From: Vafaee T, Walker F, Thomas D, Roderjan JG, Veiga Lopes S, da Costa FD, Desai A, Rooney P, Jennings LM, Fisher J, Berry HE, Ingham E.
Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
J Tissue Eng. 2022 Jun 28;13:20417314221102680.
doi: 10.1177/20417314221102680.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human macrophages, clone MAC387 (MCA874GA) used to label macrophages in ovine pulmonary root tissues by immunohistochemistry on formalin fixed cryosections.
Image caption:
(b) Images of explanted decellularised porcine root leaflets stained with antibodies to MAC 387 and CD163 at 1 (images captured at 10× magnification; scale bars 100 μm) and 3 months (images captured at 20× magnification; scale bars 50 μm). At 1 month MAC387+ cells populated the surfaces of the leaflets distally and at 3 months, the body of the leaflets. CD163+ cells populated the basal regions of the leaflets at 1 and 3 months. (c) Images of explanted ovine allograft leaflet tissues at 12 months. Images captured at 10× magnification (scale bars 100 μm) except vimentin (20× magnification; scale bar 50 μm). Images show inflammatory infiltrate with CD3+ and CTGF+ cells (two of four explants) and paucity of vimentin+ cells with MAC 387+ cell infiltrate on leaflet surface (two of four explants).
From: Vafaee T, Walker F, Thomas D, Roderjan JG, Veiga Lopes S, da Costa FD, Desai A, Rooney P, Jennings LM, Fisher J, Berry HE, Ingham E.
Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
J Tissue Eng. 2022 Jun 28;13:20417314221102680.
doi: 10.1177/20417314221102680.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label CD163 expressing cells in porcine muscle by immunofluorescence.
Image caption:
Double staining of BrdU and CD163 in a muscle cross-section of a piglet at 5 dpn. Fluorescence images of CD163 (red), BrdU (green) and Dapi nuclear stain (blue) and the merged image. Arrows indicate examples of cells that are CD163+ (red), BrdU+ (green) or CD163+/BrdU+ (white). Scale bar represents 20 μm.
From: Albrecht, E.; Zhao, Y.; Sciascia, Q.L.; Metges, C.C.; Maak, S.
Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets.
Cells 2023, 12, 580.
doi: 10.3390/cells12040580
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDhu-1 (MCA1853) used to label macrophages in biopsy sections by immunohistochemistry.
Image caption:
Immunohistochemical stainings before and 5 months after start of therapy with DMF. Original magnification: IHC × 200.
From: Gabutti M, Heidemeyer K, Seyed Jafari SM, Bossart S, Hunger RE, Feldmeyer L, Yawalkar N.
Dimethyl Fumarate Used as an Effective Treatment for Granuloma Annulare Disseminatum: An Immunohistochemical Case Study.
Int J Mol Sci. 2023 Aug 28;24(17):13355.
doi: 10.3390/ijms241713355.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCCA1853) used for the detection of macrophages in porcine liver and lung by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical localisation of ADGRE1 in pig tissues. Immunohistochemical staining was carried out on frozen pig liver (A–C) and lung (D–F) tissue. Representative images are shown (n = 3). Tissues were incubated without primary antibody (negative control A,D), antibody recognizing CD163 (positive control B,E) and monoclonal antibody ROS-4E12-3E6 recognizing ADGRE1 (C,F). Scale bar = 100 μm.
From: Waddell LA, Lefevre L, Bush SJ, Raper A, Young R, Lisowski ZM, McCulloch MEB, Muriuki C, Sauter KA, Clark EL, Irvine KM, Pridans C, Hope JC, Hume DA.
ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages.
Front Immunol. 2018 Oct 1;9:2246.
doi: 10.3389/fimmu.2018.02246.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label lapine macrophages by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of macrophage population within synovial membranes at 72h after MSU injection. (A–C) Representative images of RAM11 antigen staining in the synovium of Control (A), MSU (B) and MSU+MSC (C) groups. (D–F), Representative images of CD-163 antigen staining in the synovium of Control (D), MSU (E) and MSU+MSC (F) groups. (G–I), Representative images of arginase-1 staining in the synovium of Control (G), MSU (H) and MSU+MSC (I). Scale bar=50μm. (J) Densitometric analysis of RAM11 staining percentage, and (K) CD163 staining percentage in the SM of each group of animals. (L) Percentage of arginase-1 staining in lining layer of each group of animals. (M) Ratio of CD163 to RAM11 positive staining. (N) Ratio of Arginase-1 to RAM11 positive staining. Bars show the mean and SEM. MSC, mesenchymal stem cells; MSU, monosodium urate.
From:Medina JP, Bermejo-Álvarez I, Pérez-Baos S, Yíñez R, Fernández-García M, García-Olmo D, Mediero A, Herrero-Beaumont G, Largo R.
MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 induction.
Front Immunol. 2023 Jul 18;14:1193179.
doi: 10.3389/fimmu.2023.1193179.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label M2 macrophages in human skin by immunohistochemistry of skin biopsy sections.
Image caption:
Immunohistochemical staining of the cellular infiltrate with different leucocyte populations and proinflammatory cytokines from a skin-biopsy specimen obtained before and 1 month after initiating treatment with canakinumab. Original magnification 200 ×.
From: Bossart S, Seyed Jafari SM, Heidemeyer K, Yan K, Feldmeyer L, Borradori L, Yawalkar N.
Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome.
Front Med (Lausanne). 2023 Mar 15;10:1050230.
doi: 10.3389/fmed.2023.1050230.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to demonstrate CD163 expression on THP-1 cells in vitro by immunofluorescence.
Image caption:
THP-1 macrophages cultured on laminin-211 or -511 demonstrate distinct kinetic behavior.
(C) Morphology and immunofluorescence staining for CD206 and CD163 in THP-1 macrophages cultured on LN-211/-511/-111 and on no-laminin coating with IL-4 or IL-10. Asterisks show the cytoplasm of macrophages grown on LN-511.
From: Yoshiba N, Edanami N, Ohkura N, Maekawa T, Takahashi N, Tsuzuno T, Maeda T, Tabeta K, Izumi K, Noiri Y, Yoshiba K.
Laminin Isoforms in Human Dental Pulp: Lymphatic Vessels Express Laminin-332, and Schwann Cell-Associated Laminin-211 Modulates CD163 Expression of M2-like Macrophages.
Immunohorizons. 2021 Dec 29;5(12):1008-1020.
doi: 10.4049/immunohorizons.2100110.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to demonstrate CD163 expression on THP-1 cells in vitro by immunofluorescence.
Image caption:
Localization of CD206+ or CD163+ cells in human dental pulp.
(A–H) Healthy teeth (A–D) and representative images of active (E–G) or slowly progressing (H) carious teeth. (B) Double immunofluorescence staining of CD206 and CD163 correspondingCD206+CD163− single-positive cells on a blood vessel. (D) Double immunofluorescence staining of CD163 and laminin α2-chains (LAMA2), and CD206 and CD163 on nerve fibers. Arrows indicate CD206+CD163+ double-positive macrophages; insets show single-channel images of cells indicated by an asterisk. (F) Double immunofluorescence staining of CD206 and CD163 near the section shown in (E). (Fb and Fb′) Closer views of the boxed area in (Fa). Arrowheads indicate single-positive cells, and the arrow indicates a weakly double-positive cell. (G) Double immunofluorescence staining of CD163 and laminin α2-chains near the section shown in (F). (Gb) Closer view of the boxed area in (Ga). Arrows highlight the close association of CD163+ cells with laminin α2-chains (Ga and Gb). (H) Double immunofluorescence staining of CD206 and CD163 in a slowly progressing carious tooth. (Hb and Hb′) Closer views of the boxed area in (Ha). Arrow and arrowhead indicate CD206+CD163+ and CD206−CD163+ cells, respectively. (I) Graph showing the ratios of CD206+CD163+ double-positive/CD206−CD163+ single-positive macrophages in intact, active, or slowly progressing carious teeth. Data are presented as the mean ± SEM; n = 4 per group. ##p <0.01, one-way ANOVA and Bonferroni tests. bv, blood vessel; nf, nerve fiber; ob, odontoblast; p, dental pulp; Sc, Schwann cell. Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm except where indicated.
From: Yoshiba N, Edanami N, Ohkura N, Maekawa T, Takahashi N, Tsuzuno T, Maeda T, Tabeta K, Izumi K, Noiri Y, Yoshiba K.
Laminin Isoforms in Human Dental Pulp: Lymphatic Vessels Express Laminin-332, and Schwann Cell-Associated Laminin-211 Modulates CD163 Expression of M2-like Macrophages.
Immunohorizons. 2021 Dec 29;5(12):1008-1020.
doi: 10.4049/immunohorizons.2100110.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F P IF C WB ResetMouse anti Human CD163:StarBright Violet 790
- Product Type
- Monoclonal Antibody
- Clone
- EDHu-1
- Isotype
- IgG1
- Specificity
- CD163
Antibody Quality and Performance Guarantee
| Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone. Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment. |
Our CD163 (EDHu-1) Antibody has been referenced in >88 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Sheep Pig Guinea Pig Bovine Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 790 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 790 402 782 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD163 antibody
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Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
Nature. 409 (6817): 198-201. -
Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
Circ Res. 92: 1193-200. -
Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
J Biol Chem. 279 (49): 51561-7. -
Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
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Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
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Martens JH et al. (2006) Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
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Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
Am J Pathol. 168 (3): 822-34. -
Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood 109: 5223-9.
View The Latest Product References
-
Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
J Clin Oncol. 27: 3330-7. -
Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
Neuropathol Appl Neurobiol. 35: 82-8. -
Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
Leuk Lymphoma. 50: 1647-60. -
Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Atherosclerosis. 207: 103-10. -
Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Am J Pathol. 174: 1097-108. -
Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Arterioscler Thromb Vasc Biol. 30 (6): 1253-62. -
Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
Vet Microbiol. 145: 47-53. -
Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
Am J Pathol. 177: 1286-97. -
Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
Vet Pathol. 47: 518-28. -
Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35. -
Kong, L.Q. et al. (2013) The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
PLoS One. 8 (3): e59771. -
Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One 8: e65121. -
Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
Endocrinology 154: 471-82. -
Berglin, L. et al. (2014) In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
PLoS One 9: e105375. -
Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2: 71. -
Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel) 6: 1244-59. -
Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
PLoS Pathog. 10: e1004467. -
Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
PLoS One 10 (3): e0120895. -
Blair, T.C. et al. (2016) Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis.
J Neuroimmunol. 291: 1-10. -
Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47 (1): 2. -
Lakritz, J R. et al. (2016) α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
Am J Pathol. 186 (7): 1754-61. -
Fry, L.M. et al. (2016) East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
PLoS One 11 (5): e0156004. -
Wächter, C. et al. (2016) Loss of cerebellar neurons in the progression of lentiviral disease: effects of CNS-permeant antiretroviral therapy.
J Neuroinflammation. 13 (1): 272. -
Derricott, H. et al. (2016) Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth.
Am J Pathol. 186 (4): 952-61. -
Zhu, C. et al. (2017) Activation of CECR1 in M2-like TAMs promotes paracrine stimulation-mediated glial tumor progression.
Neuro Oncol. 19 (5): 648-59. -
Furukawa S et al. (2017) Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease.
Sci Rep. 7: 42413. -
Mallard, J. et al. (2017) A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.
J Immunol Methods. 442: 59-63. -
Farina, A. et al. (2017) Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
Arthritis Res Ther. 19 (1): 39. -
Schultz, N. et al. (2017) Amylin alters human brain pericyte viability and NG2 expression.
J Cereb Blood Flow Metab. 37 (4): 1470-82. -
Boxberg, M. et al. (2018) PD-L1 and PD-1 and characterization of tumor-infiltrating lymphocytes in high grade sarcomas of soft tissue - prognostic implications and rationale for immunotherapy.
Oncoimmunology. 7 (3): e1389366. -
Waddell, L.A. et al. (2018) ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages.
Front Immunol. 9: 2246. -
Eligini, S. et al. (2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.
Sci Rep. 9 (1): 8680. -
Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
Int J Biol Sci. 15 (2): 481-492. -
Zhao, S. et al. (2020) CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C
Experimental and Therapeutic Medicine. 20 (6): 1-1. -
Hayashi, K. et al. (2020) The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
Tohoku J Exp Med. 251 (4): 241-53. -
Pestronk, A. (2020) Chronic Graft Versus Host Myopathies: Noninflammatory, Multi-Tissue Pathology With Glycosylation Disorders.
J Neuropathol Exp Neurol. 79 (1): 102-12. -
Palaiologou, E. et al. (2020) Human placental villi contain stromal macrovesicles associated with networks of stellate cells.
J Anat. 236 (1): 132-41. -
Frafjord, A. et al. (2020) Antibody combinations for optimized staining of macrophages in human lung tumours.
Scand J Immunol. 92 (1): e12889. -
Ströbel, S. et al. (2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.
Sci Rep. 11 (1): 22765. -
Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
Pediatr Nephrol. 36 (2): 349-59. -
Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
Biomaterials. 279: 121204. -
Kemmerer, C.L. et al. (2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients
BMC Cancer. 21(1):1108. -
Lehmann, M. et al. (2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.
J Eur Acad Dermatol Venereol. 35 (11): e752-e755. -
Gonçalves, V.M. et al. (2021) Macrophage and Lymphocyte Infiltration Is Associated with Volumetric Tumor Size but Not with Volumetric Growth in the T&yyml;bingen Schwannoma Cohort.
Cancers (Basel). 13 (3): 466. -
Baumann, D. et al. (2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.
J Immunother Cancer.9 (7): e002319. -
da Silva M.C.M. et al. (2021) Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine.
Sci Rep. 11 (1): 15989. -
Ma, H. et al. (2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro..
Vet Res. 52 (1): 97. -
Holsapple, J.S. et al. (2021) Low Intensity Shockwave Treatment Modulates Macrophage Functions Beneficial to Healing Chronic Wounds.
Int J Mol Sci. 22(15):7844. -
Galea, I. et al. (2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.
Stroke. 53 (5): 1633-42. -
Bartalska, K. et al. (2022) A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation.
iScience. 25 (7): 104580. -
Pooley, H.B. et al. (2022) Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue.
Vet Immunol Immunopathol. 252: 110482. -
Rabufetti, A. et al. (2022) New onset of sarcoidosis after COVID-19 infection.
J Eur Acad Dermatol Venereol. 36 (10): e756-e759. -
Vafaee, T. et al. (2022) Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
J Tissue Eng. 13: 20417314221102680. -
Albrecht, E. et al. (2023) Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets
Cells. 12 (4): 580. -
Park, S.M. et al. (2023) Seven-colour multiplex immunochemistry/immunofluorescence and whole slide imaging of frozen sections.
J Immunol Methods. 518: 113490. -
Wu, H. et al. (2023) Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophages and bone repair via MAPK/JNK inhibition and OXPHOS activation.
Biomaterials. 293: 121990. -
Lin, C. et al. (2023) Macrophage-like rapid uptake and toxicity of tattoo ink in human monocytes.
Immunology. Nov 14 [Epub ahead of print]. -
Gabutti, M. et al. (2023) Dimethyl Fumarate Used as an Effective Treatment for Granuloma Annulare Disseminatum: An Immunohistochemical Case Study.
Int J Mol Sci. 24 (17): 13355 -
White, K.S. et al. (2023) Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis.
Front Immunol. 14: 1240946. -
Medina, J.P. et al. (2023) MSC therapy ameliorates experimental gouty arthritis hinting an early COX-2 induction.
Front Immunol. 14: 1193179. -
Bossart, S. et al. (2023) Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome.
Front Med (Lausanne). 10: 1050230. -
Lehmann, M. et al. (2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.
J Eur Acad Dermatol Venereol. 35 (11): e752-e755. -
Ruggiero, A.D. et al. (2023) Long-term dasatinib plus quercetin effects on aging outcomes and inflammation in nonhuman primates: implications for senolytic clinical trial design.
Geroscience. 45 (5): 2785-803. -
Yoshiba, N. et al. (2021) Laminin Isoforms in Human Dental Pulp: Lymphatic Vessels Express Laminin-332, and Schwann Cell-Associated Laminin-211 Modulates CD163 Expression of M2-like Macrophages.
Immunohorizons. 5 (12): 1008-20. -
Ahmed, A. et al. (2022) Tertiary lymphoid structures and their association to immune phenotypes and circulatory IL2 levels in pancreatic ductal adenocarcinoma.
Oncoimmunology. 11 (1): 2027148. -
Agulló-Ros, I. et al. (2024) Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats
Veterinary Microbiology. : 110320.
- UniProt
- Q86VB7
- Entrez Gene
- CD163
- GO Terms
- GO:0005515 protein binding
- GO:0006953 acute-phase response
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
- GO:0005887 integral to plasma membrane
MCA1853SBV790
64595340
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