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CD16/CD56 | LNK16 / MEM-188

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Mouse anti Human CD16/CD56:FITC

Product Type
Monoclonal Antibody Panel
Clone
LNK16 / MEM-188
Isotype
Cocktail
Specificity
CD16/CD56

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Product Code Applications Pack Size List Price Your Price Qty
MCA2074F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD16/CD56 antibody panel, clones LNK16, MEM-188 is a combination of two monoclonal antibodies recognising the human CD16, clone LNK16 and CD56, clone MEM-188 cell surface antigens. This combination is particularly useful for the detection of all natural killer cells due to the fact that different subsets of these cells exist that are either CD16+/CD56+, CD16+/CD56- or CD16-/CD56+.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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References for CD16/CD56

  1. Costa, P. CD56 Workshop Panel Report. In: Leucocyte typing VI. white cell differentiation antigens (1997) p. 271-272.
    Kishimoto, T. et al. Eds. Garland Publishing Inc. New York and London.
  2. Harbige, L.S. et al. (2016) Immune Response of Healthy Adults to the Ingested Probiotic Lactobacillus casei Shirota.
    Scand J Immunol. 84 (6): 353-64.

RRID
AB_323451
UniProt
O75015
P08637
P13591
Entrez Gene
FCGR3B
FCGR3A
NCAM1
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response

MCA2074F

0413 161193

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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