CD16 antibody | KD1
FITC conjugated Mouse anti Human CD16 antibody, clone KD1 (MCA5665F) used for the evaluation of CD16 expression on bovine monocytes by flow cytometry.
Image caption:
Gating strategy utilized for flow cytometric analysis of bovine peripheral blood leukocytes showing representative data from one animal. After gating on viable (propidium-iodide-negative) cells (A), cell doublets were excluded by SSC-A and SSC-H gating (B). Bovine mononuclear cells (MNC) and granulocytes (PMN) were gated based on their forward and side scatter characteristics and their percentages were calculated (C). Three-color immunofluorescence of bovine MNC with mAbs to CD172a, CD14 and CD16 defines three monocyte subsets in peripheral blood. Viable mononuclear cells, based on forward and side scatter characteristics, were gated on CD172a-positive cells (D). Dot plots of CD14 and CD16 expression display classical monocytes (CD14+CD16−, upper left), intermediate monocytes (CD14+CD16+, upper right) and nonclassical monocytes (CD14−CD16+, lower right) (E)
From: Pomeroy B, Sipka A, Hussen J, Eger M, Schukken Y, Schuberth HJ.
Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 2017 Feb 21;48(1):13.
Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (< href="http://www.ncbi.nlm.nih.gov/pubmed/?term=2137855"target="_blank">Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16.
Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).
Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Sheep Horse Weak Pig Weak Rat Dog Dolphin - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- A polyclonal population of NK cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3U1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P79107 Related reagents
- P08637 Related reagents
- O75015 Related reagents
- Entrez Gene
- FCGR3B Related reagents
- FCGR3A Related reagents
- FCGR3A Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD16 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Functional Assays 1 |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Our Slide-A-Lyzer® Dialysis Cassette (EQU003) is recommended suitable for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 100ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD16 antibody
-
Moretta, A. et al. (1989) CD16 surface molecules regulate the cytolytic function of CD3CD16+ human natural killer cells.
Int J Cancer. 44 (4): 727-30. -
Zocchi, M.R. et al. (1998) HIV-1 Tat inhibits human natural killer cell function by blocking L-type calcium channels.
J Immunol. 161: 2938-43. -
Ciccone, E. et al. (1990) Specific recognition of human CD3-CD16+ natural killer cells requires the expression of an autosomic recessive gene on target cells.
J Exp Med. 172 (1): 47-52. -
Boysen, P. et al. (2008) Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties.
Dev Comp Immunol. 32: 773-83. -
Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
J Leukoc Biol. 79: 46-58. -
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41 (1): 4. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Cantón, G.J. et al. (2013) Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.
Vet Res. 44: 60. -
Pomeroy, B. et al. (2016) Longitudinal characterization of bovine monocyte-derived dendritic cells from mid-gestation into subsequent lactation reveals nadir in phenotypic maturation and macrophage-like cytokine profile in late gestation.
J Reprod Immunol. 118: 1-8. -
Pomeroy, B. et al. (2017) Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 48 (1): 13. -
Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
Vet Immunol Immunopathol. 181: 15-23. -
Elnaggar, M.M. et al. (2017) Identification of monoclonal antibodies cross-reactive with bottlenose dolphin orthologues of the major histocompatibility complex and leukocyte differentiation molecules.
Vet Immunol Immunopathol. 192: 54-9.
