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CD16 antibody | KD1

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Anti Human CD16 Antibody, clone KD1 thumbnail image 1 Anti Human CD16 Antibody, clone KD1 thumbnail image 2 Anti Human CD16 Antibody, clone KD1 thumbnail image 3 Anti Human CD16 Antibody, clone KD1 thumbnail image 4 Anti Human CD16 Antibody, clone KD1 thumbnail image 5 Anti Human CD16 Antibody, clone KD1 thumbnail image 6 Anti Human CD16 Antibody, clone KD1 thumbnail image 7
Anti Human CD16 Antibody, clone KD1 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F) and Alexa Fluor 647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD16 Antibody, clone KD1 gallery image 2
Published customer image:
FITC conjugated Mouse anti Human CD16 antibody, clone KD1 (MCA5665F) used for the evaluation of CD16 expression on bovine monocytes by flow cytometry.
Image caption:
Gating strategy utilized for flow cytometric analysis of bovine peripheral blood leukocytes showing representative data from one animal. After gating on viable (propidium-iodide-negative) cells (A), cell doublets were excluded by SSC-A and SSC-H gating (B). Bovine mononuclear cells (MNC) and granulocytes (PMN) were gated based on their forward and side scatter characteristics and their percentages were calculated (C). Three-color immunofluorescence of bovine MNC with mAbs to CD172a, CD14 and CD16 defines three monocyte subsets in peripheral blood. Viable mononuclear cells, based on forward and side scatter characteristics, were gated on CD172a-positive cells (D). Dot plots of CD14 and CD16 expression display classical monocytes (CD14+CD16−, upper left), intermediate monocytes (CD14+CD16+, upper right) and nonclassical monocytes (CD14−CD16+, lower right) (E)

From: Pomeroy B, Sipka A, Hussen J, Eger M, Schukken Y, Schuberth HJ.
Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 2017 Feb 21;48(1):13.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD16 Antibody, clone KD1 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD11b (MCA551F) and PE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Human CD11b (MCA551F) and PE conjugated Mouse anti Human CD16 (MCA5665PE). All experiments performed on human blood gated on live single granulocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD16 Antibody, clone KD1 gallery image 4
Published customer image:
FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 (MCA5665F) used to identify monocyte populations in bovime blood samples by flow cytometry.
Image caption:
Changes in the monocyte subsets of PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify monocyte subset population. A CD172a+ monocyte cells were sorted from total PBMCs. MT indicates moderate THI condition and HT indicates high THI condition

From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD16 Antibody, clone KD1 gallery image 5
Published customer image:
FITC conjugated Mouse anti Bovine CD16 antibody, clone KD1 (MCA5665F) used to identify monocyte populations in bovime blood samples by flow cytometry.
Image caption:
Changes in the monocyte subsets of PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify monocyte subset population. A dot plot depicting the three monocyte subsets in monocytes (CD172a+): classical CD14+CD16-, intermediate CD14+CD16+, and non-classical CD14-CD16+ monocytes. MT indicates moderate THI condition and HT indicates high THI condition

From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD16 Antibody, clone KD1 gallery image 6
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647) and StarBright Violet 760 conjugated Mouse anti Human CD14 (MCA1568SBV760). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD16 Antibody, clone KD1 gallery image 7
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA5665A647) and StarBright UltraViolet 575 conjugated Mouse anti Human CD14 (MCA1568SBUV575). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD16

Product Type
Monoclonal Antibody
Clone
KD1
Isotype
IgG2a
Specificity
CD16

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Product Code Applications Pack Size List Price Your Price Qty
MCA5665
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN * 0.2 mg loader
List Price Your Price
loader
MCA5665GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN * 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16.

Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
Horse Weak
Pig Weak
Rat
Dog
Dolphin
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA5665: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA5665GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
A polyclonal population of NK cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Functional Assays 1
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 100ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD16 antibody

  1. Moretta, A. et al. (1989) CD16 surface molecules regulate the cytolytic function of CD3CD16+ human natural killer cells.
    Int J Cancer. 44 (4): 727-30.
  2. Ciccone, E. et al. (1990) Specific recognition of human CD3-CD16+ natural killer cells requires the expression of an autosomic recessive gene on target cells.
    J Exp Med. 172 (1): 47-52.
  3. Zocchi, M.R. et al. (1998) HIV-1 Tat inhibits human natural killer cell function by blocking L-type calcium channels.
    J Immunol. 161: 2938-43.
  4. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
    J Leukoc Biol. 79: 46-58.
  5. Boysen, P. et al. (2008) Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties.
    Dev Comp Immunol. 32: 773-83.
  6. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  7. Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
    Vet Immunol Immunopathol. 181: 15-23.
  8. Pomeroy, B. et al. (2016) Longitudinal characterization of bovine monocyte-derived dendritic cells from mid-gestation into subsequent lactation reveals nadir in phenotypic maturation and macrophage-like cytokine profile in late gestation.
    J Reprod Immunol. 118: 1-8.
    9. View The Latest Product References
  9. Elnaggar, M.M. et al. (2017) Identification of monoclonal antibodies cross-reactive with bottlenose dolphin orthologues of the major histocompatibility complex and leukocyte differentiation molecules.
    Vet Immunol Immunopathol. 192: 54-9.
  10. Pomeroy, B. et al. (2017) Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
    Vet Res. 48 (1): 13.
  11. Elnaggar, M.M. et al. (2019) Pattern of CD14, CD16, CD163 and CD172a expression on water buffalo (Bubalus bubalis.) leukocytes.
    Vet Immunol Immunopathol. 211: 1-5.
  12. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.
  13. Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
    Vet Immunol Immunopathol. 227: 110090.
  14. Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
    Cell Stress Chaperones. 26 (4): 705-20.
  15. Fiorenza, M.F. et al. (2021) Neutrophils recognize and amplify IFNT signals derived from day 7 bovine embryo for stimulation of ISGs expression in vitro.: A possible implication for the early maternal recognition of pregnancy.
    Biochem Biophys Res Commun. 553: 37-43.
  16. Howell, A. et al. (2024) Resting and activated bovine neutrophils and eosinophils differ in their responses to adrenergic agonists.
    Vet Immunol Immunopathol. 272: 110758.
  17. Hong, S. et al. (2024) Impact of an Injectable Trace Mineral Supplement on the Immune Response and Outcome of Mannheimia haemolytica Infection in Feedlot Cattle.
    Biol Trace Elem Res. Jun 10 [Epub ahead of print].

Flow Cytometry

Synonyms
FcRIII
RRID
AB_10964919
UniProt
P79107
P08637
O75015
Entrez Gene
FCGR3B
FCGR3A
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0031225 anchored to membrane
GO:0019864 IgG binding
GO:0016021 integral to membrane
GO:0050776 regulation of immune response

MCA5665

154513 164565 1807

MCA5665GA

164641 1808

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