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CD16 antibody | 3G8

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Anti Human CD16 Antibody, clone 3G8 thumbnail image 1
Anti Human CD16 Antibody, clone 3G8 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD56 (MCA2693A647) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B . Alexa Fluor 647 conjugated Mouse anti Human CD56 (MCA2693A647) and PE conjugated Mouse anti Human CD16 (MCA1569PE). All experiments performed on Human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD16:RPE

Product Type
Monoclonal Antibody
Clone
3G8
Isotype
IgG1
Specificity
CD16

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Product Code Applications Pack Size List Price Your Price Qty
MCA1569PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse Anti Human CD16 antibody, clone 3G8 recognizes the human CD16 cell surface antigen also known as the FcR III receptor. CD16 is expressed by granulocytes and NK cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Baboon
Chimpanzee
Pig-tailed macaque
Squirrel monkey
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Human PMN cells
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells or 100μl whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD16 antibody

  1. Audran, R. et al. (2009) The synthetic Plasmodium falciparum circumsporozoite peptide PfCS102 as a malaria vaccine candidate: a randomized controlled phase I trial.
    PLoS One 4(10): e7304.

Further Reading

  1. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.

Synonyms
FcRIII
UniProt
P08637
Entrez Gene
FCGR3A
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0019864 IgG binding
GO:0050776 regulation of immune response

MCA1569PE

147614 158456 160640

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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