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CD148 antibody | D02/2F7

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siRNA Validated
IP-MS Validated
Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CD148 antibody (1/1,000, VMA00907) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CD148 (molecular weight 250 kDa).
Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of CD148 from HeLa whole cell lysate. The Input lane is 10% input; the CD148-IP lane is the immunoprecipitation with the CD148 antibody (2.5 µg, VMA00907); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using CD148 antibody (1/1000, VMA00907) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP with 26 second exposure. Arrow points to CD148.
Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against CD148 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti CD148 antibody (VMA00907) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 162 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti CD148 Antibody, clone D02/2F7 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of CD148 from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against CD148 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti CD148

Product Type
PrecisionAb Monoclonal
Clone
D02/2F7
Isotype
IgG1
Format
Purified
Specificity
CD148

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VMA00907
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Mouse anti CD148 antibody recognizes receptor-type tyrosine-protein phosphatase eta (RPTP), also known as PTPRJ and DEP1.

CD148 is a transmembrane protein tyrosine phosphatase, a group of proteins important for regulating cell signaling pathways. CD148 expression is found on a wide range of cell types, including epithelial cells, endothelial cells, and hematopoetic cell populations. CD148 appears to function in suppression of growth factor signals, inhibiting cell growth and transformation (Takahashi et al. 2016). More specifically, CD148 may dephosphorylate proteins important for tumor growth or survival, including ERK1/2 and VEGFR. Tumor-suppressive roles of CD148 have been identified in colon, breast, and thyroid cancers. In contrast, CD148 may promote metastasis in other cancers including breast cancer and glioma cells. In gastric cancer, suppression of CD148 may be an indicator of poor outcomes, suggesting its use as a prognostic marker and therapeutic target (Sun et al. 2020).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti CD148 antibody detects a band of approximately 250 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 36-541 of human CD148
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
As supplied, 12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
Q12913
Entrez Gene
PTPRJ
GO Terms
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005911 cell-cell junction
GO:0006470 protein dephosphorylation
GO:0007267 cell-cell signaling
GO:0008013 beta-catenin binding
GO:0045295 gamma-catenin binding
GO:0070097 delta-catenin binding

VMA00907

100005037

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