CD148 antibody | D02/2F7

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Mouse anti CD148

Product Type
PrecisionAb Monoclonal
Clone
D02/2F7
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00907
Datasheet
WB
SDS
100 µl loader

Mouse anti CD148 antibody recognizes receptor-type tyrosine-protein phosphatase eta (RPTP), also known as PTPRJ and DEP1.

CD148 is a transmembrane protein tyrosine phosphatase, a group of proteins important for regulating cell signaling pathways. CD148 expression is found on a wide range of cell types, including epithelial cells, endothelial cells, and hematopoetic cell populations. CD148 appears to function in suppression of growth factor signals, inhibiting cell growth and transformation (Takahashi et al. 2016). More specifically, CD148 may dephosphorylate proteins important for tumor growth or survival, including ERK1/2 and VEGFR. Tumor-suppressive roles of CD148 have been identified in colon, breast, and thyroid cancers. In contrast, CD148 may promote metastasis in other cancers including breast cancer and glioma cells. In gastric cancer, suppression of CD148 may be an indicator of poor outcomes, suggesting its use as a prognostic marker and therapeutic target (Sun et al. 2020).

Product Details

Target Species
Human
Western Blotting
Mouse anti CD148 antibody detects a band of approximately 250 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 36-541 of human CD148
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q12913
Entrez Gene
PTPRJ
GO Terms
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005911 cell-cell junction
GO:0006470 protein dephosphorylation
GO:0007267 cell-cell signaling
GO:0008013 beta-catenin binding
GO:0045295 gamma-catenin binding
GO:0070097 delta-catenin binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of CD148 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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