CD144 antibody | 55-7H1

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Mouse anti Human CD144:RPE

Product Type: Monoclonal Antibody
Clone: 55-7H1
Isotype: IgG1
Specificity: CD144

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References (2)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA6119PE Datasheet SDS SDS	F	100 Tests/1ml		Log in
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Anti Human CD144 Antibody, clone 55-7H1 thumbnail image 1

Anti Human CD144 Antibody, clone 55-7H1 thumbnail image 2

Anti Human CD144 Antibody, clone 55-7H1 thumbnail image 3

Anti Human CD144 Antibody, clone 55-7H1 gallery image 1 — Surface staining of HUVEC cells with RPE conjugated Mouse anti Human CD144 antibody, clone 55-7H1 (**MCA6119PE**).

Anti Human CD144 Antibody, clone 55-7H1 gallery image 2 — **Published customer image:**
Mouse anti Human CD144 antibody, clone 55-7H1 (**MCA6119**) used to identify VE-Cadherin expression on endothelial cells by immunofluorescence.
**Image caption:**
Viability, confluence and quantification of ECs adherent to HFM. Viable ECs on AH (A) and FN (B) coated HFM were visualized using calcein staining (green); Scale: 200 μm, VE-cadherin (green) and Hoechst (blue) indicated intercellular connection between ECs on AH (C) and FN (D) coated HFM; Scale: 100 μm. (E) Quantification of adherent ECs on AH and FN coated HFM, ECs on non-coated (NC) HFM were not detectable (N.D.).

From: Pflaum M, Jurmann S, Katsirntaki K, Mälzer M, Haverich A, Wiegmann B.
Towards Biohybrid Lung Development—Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
**Membranes. 2022; 12(1):35**.
doi: 10.3390/membranes12010035
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Human CD144 Antibody, clone 55-7H1 gallery image 3 — **Published customer image:**
Mouse anti Human CD144 antibody, clone 55-7H1 (**MCA6119**) used to stain endothelial cells *in vitro* by immunofluorescence.
**Image caption:**
Confluent EML on HFMs remained unaffected after medium exchange during 24 h rotational seeding.CLSM images for the detection of VE-cadherin (magenta) on HFM samples cultivated without (E) or with MExch (F). Nuclei were counterstained with Hoechst 33342 (blue). Scale: 200 μm.

From: Alabdullh, H.A.; Pflaum, M.; Mälzer, M.; Kipp, M.; Naghilouy-Hidaji, H.; Adam, D.; Kühn, C.; Natanov, R.; Niehaus, A.; Haverich, A.; Wiegmann, B.
Biohybrid Lung Development: Towards Complete Endothelialization of an Assembled Extracorporeal Membrane Oxygenator.
**Bioengineering 2023, 10, 72**.
doi: 10.3390/bioengineering10010072
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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F IF

Mouse anti Human CD144, clone 55-7H1 recognizes CD144, also known as cadherin 5, VE-cadherin or 7B4 antigen. CD144 is a 737 amino acid, ~130 kDa single pass type 1 transmembrane glycoprotein involved in cellular adhesion processes and is expressed by endothelial cells. CD 144 has a 25 amino acid signal peptide and a 22 amino acid pro-peptide region.

Target Species: Human
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Immunogen: Human endothelial cells
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578

RPE 561nm laser 546 578
Regulatory: For research purposes only
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the undiluted reagent to label 1x10⁶ cells in 100ul

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Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:RPE	MCA928PE	F	100 Tests	Log in
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Description	Mouse IgG1 Negative Control:RPE

References for CD144 antibody

Pflaum, M. et al. (2021) Towards Biohybrid Lung Development-Fibronectin-Coating Bestows Hemocompatibility of Gas Exchange Hollow Fiber Membranes by Improving Flow-Resistant Endothelialization.
Membranes (Basel). 12 (1): 35.
Alabdullh, A.H. et al. (2023) Biohybrid Lung Development: Towards Complete Endothelialization of an Assembled Extracorporeal Membrane Oxygenator
Bioengineering. 10 (1): 72.

Synonyms: VE-CADHERIN
UniProt: P33151
Entrez Gene: CDH5
GO Terms: GO:0005509 calcium ion binding; GO:0005102 receptor binding; GO:0016021 integral to membrane; GO:0005624 membrane fraction; GO:0007156 homophilic cell adhesion; GO:0008013 beta-catenin binding; GO:0034329 cell junction assembly; GO:0034332 adherens junction organization; GO:0044325 ion channel binding; View More GO Terms