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CD14 antibody | 61D3

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Mouse anti Human CD14:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
61D3
Isotype
IgG1
Specificity
CD14

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Product Code Applications Pack Size List Price Your Price Qty
MCA2804C
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human CD14 antibody, clone 61D3 recognizes human CD14, otherwise known as monocyte differentiation antigen. It is a ~40 kDa protein found on cell surfaces, particularly macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) to mediate the innate immune response to bacterial lipopolysaccharide.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Dog
Rabbit
Sheep
Pig
Cynomolgus monkey
Goat
Cat
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Cy5 - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE-Cy5 488nm laser 496 667
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

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References for CD14 antibody

  1. Balasubramanian, K. & Schroit, A.J. (1998) Characterization of phosphatidylserine-dependent beta2-glycoprotein I macrophage interactions. Implications for apoptotic cell clearance by phagocytes.
    J Biol Chem. 273 (44): 29272-7.
  2. Giles, K.M. et al. (2001) Glucocorticoid augmentation of macrophage capacity for phagocytosis of apoptotic cells is associated with reduced p130Cas expression, loss of paxillin/pyk2 phosphorylation, and high levels of active Rac.
    J Immunol. 167: 976-86.
  3. Henriksen, P.A. et al. (2004) Gene delivery of the elastase inhibitor elafin protects macrophages from neutrophil elastase-mediated impairment of apoptotic cell recognition.
    FEBS Lett. 574: 80-4.
  4. Gagro, A. et al. (2006) Type I cytokine profiles of human naïve and memory B lymphocytes: a potential for memory cells to impact polarization.
    Immunology. 118 (1): 66-77.
  5. Amorim, I.F. et al. (2011) Toll receptors type-2 and CR3 expression of canine monocytes and its correlation with immunohistochemistry and xenodiagnosis in visceral leishmaniasis.
    PLoS One. 6: e27679.
  6. Eleftheriou D et al. (2012) Endothelial injury in childhood stroke with cerebral arteriopathy: a cross-sectional study.
    Neurology. 79 (21): 2089-96.
  7. Raghuraman, S. et al. (2012) Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
    J Infect Dis. 205: 763-71.
  8. Werner, J.M. et al. (2013) Innate immune responses in hepatitis C virus-exposed healthcare workers who do not develop acute infection.
    Hepatology. 58 (5): 1621-31.
    9. View The Latest Product References
  9. Serti, E. et al. (2015) Successful Interferon-Free Therapy of Chronic Hepatitis C Virus Infection Normalizes Natural Killer Cell Function.
    Gastroenterology. 149 (1): 190-200.e2.
  10. Santos, B.P. et al. (2017) Blood and milk polymorphonuclear leukocyte and monocyte/macrophage functions in naturally caprine arthritis encephalitis virus infection in dairy goats.
    Vet Immunol Immunopathol. 188: 21-6.
  11. Santos, B.P. et al. (2017) Blood and milk polymorphonuclear leukocyte and monocyte/macrophage functions in naturally caprine arthritis encephalitis virus infection in dairy goats.
    Vet Immunol Immunopathol. 188: 21-6.

Flow Cytometry

Functional Assays

RRID
AB_1100736
UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid

MCA2804C

155679 156320 157868 159914 162773 162814 164665 167365

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