CD123 antibody | 6H6

Mouse anti Human CD123

Product Type
Monoclonal Antibody
Clone
6H6
Isotype
IgG1
Specificity
CD123

Product Code Applications Pack Size List Price Your Price Qty
MCA1263GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.1 mg loader
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loader
MCA1263T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD123 antibody, clone 6H6 recognizes the human interleukin-3 (IL-3) receptor alpha chain, also known as CD123. Mouse anti Human CD123 antibody, clone 6H6 is useful for monitoring expression of the IL-3 receptor on activated endothelial cells, monocytes and eosinophils, sorting bone marrow progenitor cells and purification of basophils.

Mouse anti Human CD123 antibody, clone 6H6 does not neutralize the activity of the IL-3 receptor alpha chain.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 25 µg
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 0.5 mg/ml
Pack Size: 25 µg
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 0.1 mg
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 µg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat Pack Size: 0.1 mg
1/5 Pack Size: 25 µg
1/5 Pack Size: 0.1 mg
1/10 Pack Size: 25 µg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD123 antibody

  1. Shimizu, Y. et al. (2008) Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
    Immunol Invest.37: 1-17
  2. Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
    Br J Dermatol. 165: 321-8.

Further Reading

  1. Korpelainen, E.I. et al. (1993) The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.
    Proc Natl Acad Sci U S A. 90 (23): 11137-41.

Synonyms
Interleukin 3 Receptor Alpha
RRID
AB_2124260
UniProt
P26951
Entrez Gene
IL3RA
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004912 interleukin-3 receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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