CD123 antibody | 6H6

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Mouse anti Human CD123:FITC

Mouse anti Human CD123

Mouse anti Human CD123:RPE

Product Type: Monoclonal Antibody
Clone: 6H6
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1263F	F	100 Tests/0.5ml
MCA1263T	C F IP WB	25 µg
MCA1263GA	C F IP WB	0.1 mg
MCA1263PE	F	100 Tests/0.5ml

Mouse anti Human CD123 antibody, clone 6H6 recognizes the human interleukin-3 (IL-3) receptor alpha chain, also known as CD123. Mouse anti Human CD123 antibody, clone 6H6 is useful for monitoring expression of the IL-3 receptor on activated endothelial cells, monocytes and eosinophils, sorting bone marrow progenitor cells and purification of basophils.

Mouse anti Human CD123 antibody, clone 6H6 does not neutralize the activity of the IL-3 receptor alpha chain.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Preservative Stabilisers: Pack Size: 25 µg
0.09% Sodium Azide (NaN₃)

Pack Size: 0.1 mg
0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Approx. Protein Concentrations: Pack Size: 25 µg
IgG concentration 0.5mg/ml

Pack Size: 0.1 mg
IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Pack Size: 25 µg
Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 0.1 mg
Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of despatch

More Information

UniProt: P26951 Related reagents
Entrez Gene: IL3RA Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0004912 interleukin-3 receptor activity
Regulatory: For research purposes only

Applications of CD123 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry	1/5 Pack Size: 25 µg Neat Pack Size: 0.1 mg	1/10 Pack Size: 25 µg 1/5 Pack Size: 0.1 mg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5ul of the suggested working dilution to label 1 x 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 10⁶ cells in 100ul.
Flow Cytometry: Use 5μl of the suggested working dilution to label 1 x 10⁶ cells in 100μl

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Product Specific References

References for CD123 antibody

Korpelainen, E.I. et al. (1993) The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.
Proc Natl Acad Sci U S A. 90 (23): 11137-41.
Shimizu, Y. et al. (2008) Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
Immunol Invest.37: 1-17
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8.

Request a different product with the CD123 specificity