CD123 antibody | 6H6
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Mouse anti Human CD123
- Product Type
- Monoclonal Antibody
- Clone
- 6H6
- Isotype
- IgG1
- Specificity
- CD123
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD123 antibody, clone 6H6 recognizes the human interleukin-3 (IL-3) receptor alpha chain, also known as CD123. Mouse anti Human CD123 antibody, clone 6H6 is useful for monitoring expression of the IL-3 receptor on activated endothelial cells, monocytes and eosinophils, sorting bone marrow progenitor cells and purification of basophils. Mouse anti Human CD123 antibody, clone 6H6 does not neutralize the activity of the IL-3 receptor alpha chain. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.1 mg0.09% sodium azide (NaN3)Pack Size: 25 µg<0.1% sodium azide (NaN3)
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.5 mg/mlPack Size: 25 µgIgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Pack Size: 0.1 mg
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 µg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat Pack Size: 0.1 mg 1/5 Pack Size: 25 µg |
1/5 Pack Size: 0.1 mg 1/10 Pack Size: 25 µg |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD123 antibody
-
Shimizu, Y. et al. (2008) Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
Immunol Invest.37: 1-17 -
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8.
Further Reading
-
Korpelainen, E.I. et al. (1993) The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.
Proc Natl Acad Sci U S A. 90 (23): 11137-41.
- Synonyms
- Interleukin 3 Receptor Alpha
- RRID
- AB_2124260
- UniProt
- P26951
- Entrez Gene
- IL3RA
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004912 interleukin-3 receptor activity
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