CD101 antibody | BB27
Mouse anti Human CD101:FITC
- Product Type
- Monoclonal Antibody
- Clone
- BB27
- Isotype
- IgG1
- Specificity
- CD101
Antibody Quality and Performance Guarantee
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F Reset| Mouse anti Human CD101 antibody, clone BB27 recognizes human CD101, also known as Immunoglobulin superfamily member 2 (IgSF2), . Cell surface glycoprotein V7, Glu-Trp-Ile EWI motif-containing protein 101 or EWI-101. CD101 is a 1021 amino acid, including a 20 amino acid signal peptide, ~140 kDa single pass type I homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. CD101 plays a major role in the activation of T cells by skin dendritic cells. Mouse anti Human CD101 antibody, clone BB27 has been reported to inhibit allogeneic T-cell responses (Bagot et al. 1997). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human thymic clone B12.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cell in 100μl.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
Source Reference
-
Gouttefangeas, C. et al. (1994) Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.
Int Immunol. 6 (3): 423-30.
References for CD101 antibody
-
Bagot, M. et al. (1997) CD101 is expressed by skin dendritic cells. Role in T-lymphocyte activation.
Tissue Antigens. 50 (5): 439-48. -
Grassi, F. et al. (1998) Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules.
J Leukoc Biol. 64 (4): 484-93. -
Jovanovic, D.V. et al. (2011) CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages.
J Rheumatol. 38: 419-28. -
Ohradanova-Repic, A. et al. (2016) Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel.
Clin Transl Immunology. 5 (1): e55. -
Okuno, M. et al. (2017) Nucleotide substitutions in CD101, the human homolog of a diabetes susceptibility gene in non-obese diabetic mouse, in patients with type 1 diabetes.
J Diabetes Investig. 8 (3): 286-94.
- RRID
- AB_324108
- UniProt
- Q93033
- Entrez Gene
- CD101
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
MCA2236F
1607
169577
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