CD10 antibody | SN5c
Filter by Application:
F ResetMouse anti Human CD10:FITC
- Product Type
- Monoclonal Antibody
- Clone
- SN5c
- Isotype
- IgG1
- Specificity
- CD10
Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes. Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T ( |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- MCA1556FT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1556F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Partially purified cell membrane antigens from fresh leukemia cells
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Matsuzaki, H. et al. (1987) Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.
Cancer Res. 47 (8): 2160-6.
References for CD10 antibody
-
Biddle, W.C. et al. (1989) In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.
Leuk Res. 13 (8): 699-707. -
Riemann, D. et al. (2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.
Biochem J. 354 (Pt 1): 47-55. -
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
J Cell Physiol. 214: 75-83. -
Pilling, D. et al. (2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.
PLoS One. 4 (10): e7475. -
Manini, I. et al. (2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.
Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print]. -
Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.
Cytometry B Clin Cytom. 98 (1): 88-98. -
Caponnetto, F. et al. (2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.
Cells. 10 (1): 44. -
Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil priming
bioRxiv. 24 Feb [Epub ahead of print].
View The Latest Product References
- Synonyms
- CALLA
- RRID
- AB_321495
- UniProt
- P08473
- Entrez Gene
- MME
- GO Terms
- GO:0005515 protein binding
- GO:0004222 metalloendopeptidase activity
- GO:0005887 integral to plasma membrane
- GO:0046872 metal ion binding
- GO:0006508 proteolysis
- GO:0007267 cell-cell signaling
MCA1556FT
151879MCA1556F
151878 1604If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up