Catalase antibody | OTI1B6
Mouse anti Catalase
Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.
Catalase Antibody with Control Lysate
Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1B6
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00129 | WB | 100 µl | ||||
| VMA00129KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human catalase antibody recognizes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress.
Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. It converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in the CAT gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by catalase (provided by RefSeq, Oct 2009).
Mouse anti Human catalase antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. It converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in the CAT gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by catalase (provided by RefSeq, Oct 2009).
Mouse anti Human catalase antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human catalase (NP_111743) produced in HEK293T cells
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P04040 Related reagents
- Entrez Gene
- CAT Related reagents
- GO Terms
- GO:0004096 catalase activity
- GO:0005778 peroxisomal membrane
- GO:0005782 peroxisomal matrix
- GO:0006144 purine base metabolic process
- GO:0006195 purine nucleotide catabolic process
- GO:0009650 UV protection
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0042803 protein homodimerization activity
- GO:0043066 negative regulation of apoptosis
- GO:0050661 NADP binding
- GO:0051262 protein tetramerization
- GO:0051781 positive regulation of cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Catalase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti catalase detects a band of approximately 60 kDa in K562 cell lysates.
- Western Blotting
- Anti catalase detects a band of approximately 60 kDa in K562 cell lysates.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
