Catalase antibody | OTI1B6

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Mouse anti Catalase

Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Catalase Antibody with Control Lysate

Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1B6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00129 WB 100 µl
VMA00129KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human catalase antibody recognizes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress.

Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. It converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in the CAT gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by catalase (provided by RefSeq, Oct 2009).

Mouse anti Human catalase antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human catalase (NP_111743) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P04040 Related reagents
Entrez Gene
CAT Related reagents
GO Terms
GO:0004096 catalase activity
GO:0005778 peroxisomal membrane
GO:0005782 peroxisomal matrix
GO:0006144 purine base metabolic process
GO:0006195 purine nucleotide catabolic process
GO:0009650 UV protection
GO:0020037 heme binding
GO:0042744 hydrogen peroxide catabolic process
GO:0042803 protein homodimerization activity
GO:0043066 negative regulation of apoptosis
GO:0050661 NADP binding
GO:0051262 protein tetramerization
GO:0051781 positive regulation of cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Catalase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti catalase detects a band of approximately 60 kDa in K562 cell lysates.
Western Blotting
Anti catalase detects a band of approximately 60 kDa in K562 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB