Catalase antibody | OTI1B6

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Anti Catalase Antibody, clone OTI1B6 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with catalase antibody (**VMA00129**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 5 second exposure. Arrow points to catalase (molecular weight 60 kDa).

Mouse anti Catalase

Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Catalase Antibody with Control Lysate

Catalase antibody recognizes the catalase protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1B6
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00129	WB	100 µl
VMA00129KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human catalase antibody recognizes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress.

Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. It converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in the CAT gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by catalase (provided by RefSeq, Oct 2009).

Mouse anti Human catalase antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti catalase detects a band of approximately 60 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human catalase (NP_111743) produced in HEK293T cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P04040 Related reagents
Entrez Gene: CAT Related reagents
GO Terms: GO:0004096 catalase activity; GO:0005778 peroxisomal membrane; GO:0005782 peroxisomal matrix; GO:0006144 purine base metabolic process; GO:0006195 purine nucleotide catabolic process; GO:0009650 UV protection; GO:0020037 heme binding; GO:0042744 hydrogen peroxide catabolic process; GO:0042803 protein homodimerization activity; GO:0043066 negative regulation of apoptosis; GO:0050661 NADP binding; GO:0051262 protein tetramerization; GO:0051781 positive regulation of cell division
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Catalase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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