CAPZA1 antibody | OTI2G4
Mouse anti CAPZA1
CAPZA1 antibody recognizes F-actin-capping protein subunit alpha-1, also known as Cap Z or CapZ-alpha 1. This antibody has been extensively validated for western blotting using whole cell lysates.
CAPZA1 Antibody with Control Lysate
CAPZA1 antibody recognizes F-actin-capping protein subunit alpha-1, also known as Cap Z or CapZ-alpha 1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2G4
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00110 | WB | 100 µl | ||||
| VMA00110KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CAPZA1 antibody recognizes capZ alpha-1 (CAPZA1), also known as Cap Z and F-actin-capping protein subunit alpha-1.
CapZ alpha-1 is a member of the F-actin capping protein alpha subunit family and regulates growth of the actin filament by capping the barbed end of growing actin filaments (provided by RefSeq, Jul 2008).
Mouse anti Human CAPZA1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
CapZ alpha-1 is a member of the F-actin capping protein alpha subunit family and regulates growth of the actin filament by capping the barbed end of growing actin filaments (provided by RefSeq, Jul 2008).
Mouse anti Human CAPZA1 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CAPZA1 detects a band of approximately 33 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human CAPZA1(NP_006126) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P52907 Related reagents
- Entrez Gene
- CAPZA1 Related reagents
- GO Terms
- GO:0003779 actin binding
- GO:0030036 actin cytoskeleton organization
- GO:0007596 blood coagulation
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0006461 protein complex assembly
- GO:0006928 cellular component movement
- GO:0008290 F-actin capping protein complex
- GO:0045087 innate immune response
- GO:0051693 actin filament capping
- GO:0071203 WASH complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CAPZA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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