CALD1 antibody | RM396

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Rabbit anti CALD1

Product Type
Monoclonal Antibody
Clone
RM396
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6361
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti CALD1 antibody recognizes caldesmon, also known as CDM and CAD.

CALD1 is an actin-binding protein which is also able to bind myosin, calmodulin and tropomyosin, and inhibits the actin-activated myosin ATPase by blockin actin-myosin interaction. As a "side-binder" of actin, CALD1 stabilizes the filamentous structure of actin and is therefore profoundly involved with assembly and organization of the actin cytoskeleton (Wang 2008). CALD1 negatively regulates cell proliferation and migration and release of metalloproteinase, and is itself regulated by a range of kinases at multiple phosphorylation sites (Hai 2015). It is found in smooth muscle and non-muscle cells. In smooth muscle, CALD1 appears to regulate smooth muscle contraction and cell motility (Eves et al. 2006). CALD1 has been observed to be downregulated in many cancer cell types and has been identified as a potent suppressor of cell invasion, regulating podosome/invadopodium formation of cancer cells (Yoshio et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of human CALD1. This antibody may also react to mouse or rat CALD1, as predicted by immunogen homology

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q05682
Entrez Gene
CALD1
GO Terms
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005516 calmodulin binding
GO:0005523 tropomyosin binding
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0017022 myosin binding
GO:0030016 myofibril
Regulatory
For research purposes only

Applications of CALD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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