Rabbit anti CALD1 antibody
recognizes caldesmon, also known as CDM and CAD.
CALD1 is an actin-binding protein which is also able to bind myosin, calmodulin and tropomyosin, and inhibits the actin-activated myosin ATPase by blockin actin-myosin interaction. As a "side-binder" of actin, CALD1 stabilizes the filamentous structure of actin and is therefore profoundly involved with assembly and organization of the actin cytoskeleton (Wang 2008
). CALD1 negatively regulates cell proliferation and migration and release of metalloproteinase, and is itself regulated by a range of kinases at multiple phosphorylation sites (Hai 2015
). It is found in smooth muscle and non-muscle cells. In smooth muscle, CALD1 appears to regulate smooth muscle contraction and cell motility (Eves et al. 2006
). CALD1 has been observed to be downregulated in many cancer cell types and has been identified as a potent suppressor of cell invasion, regulating podosome/invadopodium formation of cancer cells (Yoshio et al. 2007
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CALD1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.