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c-Myc antibody | 9E10

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Anti c-Myc Antibody, clone 9E10 thumbnail image 1 Anti c-Myc Antibody, clone 9E10 thumbnail image 2 Anti c-Myc Antibody, clone 9E10 thumbnail image 3 Anti c-Myc Antibody, clone 9E10 thumbnail image 4 Anti c-Myc Antibody, clone 9E10 thumbnail image 5 Anti c-Myc Antibody, clone 9E10 thumbnail image 6 Anti c-Myc Antibody, clone 9E10 thumbnail image 7
Anti c-Myc Antibody, clone 9E10 gallery image 1
c-myc tagged protein detected with Mouse anti c-myc:Alk Phos (MCA2200A)
Anti c-Myc Antibody, clone 9E10 gallery image 2
Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody (MCA2200) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence.
Anti c-Myc Antibody, clone 9E10 gallery image 3
Published customer image:
Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 (MCA2200A647) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression.
Image caption:
Expansion of specific iNKR subsets in decidual NK cells compared with matched pbNK. The expression of KIR2DL1, KIR2DL3/L2/S2, KIR3DL1, LILRB1, and NKG2A was examined by nine-color flow cytometry in matched pbNK and dNK from 21 donors. (A) Thirty-two distinct NK subsets were distinguished on the basis of the five iNKR in paired samples of pbNK and dNK (black and red graphs, respectively). The frequency of each subset from one representative donor is shown as a percentage of total NK cells. This provides a “fingerprint” of the iNKR repertoire, which is characteristic for each donor. The receptor combination for each subset is denoted by black-filled circles, with total number of iNKR in each subset indicated beneath. All NKG2A+ subsets are grouped to the right. The donor shown was genotyped as C1/C1. (B) The proportion of pbNK and dNK that express specific receptor combinations is shown as a percentage of total NK cells (mean ± SD, n = 21 matched pbNK and dNK pairs). The proportion of dNK that express two or more iNKR is significantly higher than in pbNK. (C) The percentage of dNK cells that are positive for Ki-67 was determined in each iNKR subset by intracellular staining. Control staining used isotype-matched mAb. The NK cells shown were gated on total KIR2DL1+ dNK as shown in Fig. 1A. (D) The percentages of dNK that are positive by intracellular staining for Ki-67 (•) and for CD122 (▴) are shown for all NKG2A+ dNK subsets (mean for each subset indicated by • or ▴ ± SD, derived from n = 11 donors). (E) Mean fluorescence intensity (MFI) of staining of each subset is shown following staining for CD122 and c-Myc in the NKG2A+ dNK subsets from (D). For each donor, MFI of each subset was expressed relative to the subset expressing NKG2A alone, which was set at 100%. Relative MFI of CD122 and c-Myc is highly correlated (Spearman rank correlation coefficient, R2 = 0.84, p <0.001).

From: Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A.
Tissue-Specific Education of Decidual NK Cells.
J Immunol. 2015 Oct 1;195(7):3026-32.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti c-Myc Antibody, clone 9E10 gallery image 4
Published customer image:
Mouse anti c-myc antibody, clone 9E10 (MCA2200) used for the detection of C-myc tagged Chs3versions derived from spheroblasts by western blotting
Image caption:
Topology determination of Chs3 using Proteinase K experiments with different myc-epitope tagged versions of Chs3. Cells expressing Chs313myc or different Chs33myc versions were converted into spheroplasts and treated with or without proteinase K in the presence or absence of Triton X-100. TCA precipitated proteins were solubilized and analyzed by sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) and Western blots using either: α-Chs3 or α-myc (A); and α-phosphofructokinase (PFK) (B) for immunostaining. Detection of PFK serves as a control for the integrity of the spheroplasts. Standard proteins are indicated with molecular masses given in kDa.

From: Gohlke S, Muthukrishnan S, Merzendorfer H.
In Vitro and In Vivo Studies on the Structural Organization of Chs3 from Saccharomyces cerevisiae.
Int J Mol Sci. 2017 Mar 25;18(4). pii: E702.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti c-Myc Antibody, clone 9E10 gallery image 5
Figure A. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD49f (MCA699F) and Alexa Fluor conjugated Rat anti Human C-myc (MCA2200A647). All experiments performed on live single Hela cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti c-Myc Antibody, clone 9E10 gallery image 6
Published customer image:
Mouse anti c-myc antibody, clone 9E10 (MCA2200) used for the detection of c-myc tagged HeLa cells by immunofluorescence.
Image caption:
siRNA-approach to identify the import receptor for hTERT.
HeLa cells were treated with a control siRNA or with siRNAs against importin β, transportin, importin 9 or importin 7 and transfected with plasmids coding for hTERT-GFP, myc-EZI, NES-GFP2-M9 or NES-GFP2-NLS, as indicated. Cells were fixed and analyzed by fluorescent microscopy. EZI was detected using an antibody against the myc-tag. Bars, 10 μm.

From: Frohnert C, Hutten S, Wälde S, Nath A, Kehlenbach RH (2014)
Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase.
PLoS ONE 9(2): e88887.
DOI: 10.1371/journal.pone.0088887
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti c-Myc Antibody, clone 9E10 gallery image 7
Published customer image:
Mouse anti c-myc antibody, clone 9E10 (MCA2200) used for the detection of c-myc tagged HeLa cells by immunofluorescence.
Image caption:
Importin 7 functions as an import receptor for hTERT.
Overexpression of exogenous importin 7 rescues nuclear import of hTERT-GFP. HeLa cells were treated with control siRNAs or with siRNAs against importin 7 and transfected with plasmids coding for either myc-EZI or hTERT-GFP alone or together with a plasmid coding for HA-importin 7, as indicated. Cells were fixed and analyzed by fluorescence microscopy. myc-EZI and HA-importin 7 were detected using specific antibodies against the myc- and HA-tag, respectively. Bars, 10 μm or 20 μm (control in C).

From: Frohnert C, Hutten S, Wälde S, Nath A, Kehlenbach RH (2014)
Importin 7 and Nup358 Promote Nuclear Import of the Protein Component of Human Telomerase.
PLoS ONE 9(2): e88887.
DOI: 10.1371/journal.pone.0088887
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti c-Myc:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
9E10
Isotype
IgG1
Specificity
c-Myc

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MCA2200A647
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Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).

Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Epitope tag
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
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Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for c-Myc antibody

  1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Mol Cell Biol. 5 (12): 3610-6.
  2. Spandidos, D.A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
    Anticancer Res. 7 (6): 1299-304.
  3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
    Microb Cell Fact. 9: 74.
  4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.
    Biochem J. 404: 23-9.
  5. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
    Protein Eng. 14: 803-6.
  6. Gohlke, S. et al. (2017) In Vitro and In Vivo Studies on the Structural Organization of Chs3 from Saccharomyces cerevisiae.
    Int J Mol Sci. 18 (4): pii: E702.
  7. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.
    J Immunol. 184: 6359-66.
  8. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.
    J Biol Chem. 285: 26878-88.
    9. View The Latest Product References
  9. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
    Blood. 106: 2936-43.
  10. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.
    Mol Biol Cell. 21: 2996-3006.
  11. Rowshanravan, B. et al. (2014) RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis.
    J Cell Sci. 127: 2849-61.
  12. Taylor K et al. (2015) Nanocell targeting using engineered bispecific antibodies.
    MAbs. 7 (1): 53-65.
  13. Sharkey, A.M. et al. (2015) Tissue-Specific Education of Decidual NK Cells.
    J Immunol. 195 (7): 3026-32.
  14. Frohnert, C. et al. (2014) Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
    PLoS One. 9 (2): e88887.
  15. Hage, N. et al. (2015) Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag.
    Protein Expr Purif. 106: 25-30.
  16. Mann, J.K. & Park, S. (2015) Epitope-Specific Binder Design by Yeast Surface Display.
    Methods Mol Biol. 1319: 143-54.
  17. Paraskevopoulou, V. et al. (2019) Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori.
    Protein Expr Purif. 163: 105446.
  18. Lim, H.K. et al. (2010) Flow cytometric analysis of genetic FRET detectors containing variable substrate sequences.
    Biotechnol Prog. 26 (6): 1765-71.
  19. Walker, L.M. et al. (2009) Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.
    J Mol Biol. 389 (2): 365-75.
  20. Matos, J. et al. (2013) Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation.
    Cell Rep. 4 (1): 76-86.
  21. Paraskevopoulou, V. et al. (2020) Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori.
    Curr Res Struct Biol. 15 Dec [Epub ahead of print].
  22. Kalusche, S. et al. (2020) Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis?
    Vaccines (Basel). 8 (4) Dec 13 [Epub ahead of print].
  23. Hollandsworth, H.M. et al. (2020) Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.
    Transl Oncol. 13 (12): 100857.
  24. Paraskevopoulou, V. et al. (2021) Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori.
    Curr Res Struct Biol. 3: 19-29.
  25. Low, S. et al. (2020) VHH antibody targeting the chemokine receptor CX3CR1 inhibits progression of atherosclerosis.
    MAbs. 12 (1): 1709322.

Further Reading

  1. Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.
    Oncogene. 18: 3004-16.
  2. Krauß, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
    Proteins. 73: 552-65.

Flow Cytometry

Immunofluorescence

Western Blotting

RRID
AB_566936
UniProt
P01106
Entrez Gene
MYC
GO Terms
GO:0005515 protein binding
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006879 cellular iron ion homeostasis
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0032204 regulation of telomere maintenance
GO:0070888 E-box binding
GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation

MCA2200A647

0414 154995

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