Braf antibody | RM308

Rabbit anti Braf

Product Type
Monoclonal Antibody
Clone
RM308
Isotype
IgG
Specificity
Braf

Product Code Applications Pack Size List Price Your Price Qty
MCA6328
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SDS Safety Datasheet SDS
P WB 0.1 ml loader
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Rabbit anti Human BRAF antibody recognizes serine/threonine-protein kinase B-raf, also known as p94, RAFB1. BRAF is a serine-threonine kinase activated by Ras (Ascierto et al. 2012). BRAF activates the MAP kinase / ERK signaling pathway, which results in activation of transcription factors important for cell growth, proliferation, and survival (McCain 2013). BRAF is a "driver oncogene", frequently activated by point mutation in human cancer. For example, BRAF mutations have been identified in 50% of skin melanomas (Mackiewicz and Mackiewicz 2018), and also occur in colorectal cancer, papillary thyroid cancer, and non-small cell lung cancers (Halpenny et al. 2017). BRAF inhibitors vemurafenib and dabrafenib have been developed as treatments for BRAF-mutant melanoma (Fedorenko et al. 2015).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the residues near N-terminus of Human BRAF
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/200 1/500
Western Blotting 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P15056
Entrez Gene
BRAF
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007264 small GTPase mediated signal transduction
GO:0007268 synaptic transmission
GO:0009887 organ morphogenesis
GO:0048011 nerve growth factor receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products