Rabbit anti Human aurora-B kinase antibody recognizes aurora kinase B (AURKB), also known as AIK2, AIM1, STK1. The Aurora kinases are serine/threonine kinases that regulate chromosomal alignment and segregation during mitosis and meiosis. Aurora B kinase interacts with INCENP and Survivin, and is involved with control of cytokinesis and chromosome bi-orientation during mitosis (Shannon and Salmon 2002). Accordingly, abundance of Aurora B kinase is regulated by the cell cycle and localizes to the centromeres from the prophase to metaphase-anaphase transition (Yu et al. 2017). The tumor suppressor p53, known as the "guardian" of the cell cycle, can be phosphorylated and degraded by Aurora B kinase (Gully et al. 2012). Aurora B is overexpressed in many primary tumors, may contribute to cancer pathogenesis through tetraploidy and genetic instability (Goldenson and Crispino 2015), and has been linked to poor prognosis. Aurora B kinase inhibitors have been suggested as anti-cancer treatments (Yu et al. 2017).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to the N-terminus of Human aurora-B kinase
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
- 12 months from date of despatch
- Entrez Gene
- GO Terms
condensed nuclear chromosome, centromeric region
protein serine/threonine kinase activity
metal ion binding
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
chromosome passenger complex
protein localization to kinetochore
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Aurora-B Kinase antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra