Aurora-B Kinase antibody | RM278

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Rabbit anti Aurora-B Kinase

Product Type
Monoclonal Antibody
Clone
RM278
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6320
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human aurora-B kinase antibody recognizes aurora kinase B (AURKB), also known as AIK2, AIM1, STK1. The Aurora kinases are serine/threonine kinases that regulate chromosomal alignment and segregation during mitosis and meiosis. Aurora B kinase interacts with INCENP and Survivin, and is involved with control of cytokinesis and chromosome bi-orientation during mitosis (Shannon and Salmon 2002). Accordingly, abundance of Aurora B kinase is regulated by the cell cycle and localizes to the centromeres from the prophase to metaphase-anaphase transition (Yu et al. 2017). The tumor suppressor p53, known as the "guardian" of the cell cycle, can be phosphorylated and degraded by Aurora B kinase (Gully et al. 2012). Aurora B is overexpressed in many primary tumors, may contribute to cancer pathogenesis through tetraploidy and genetic instability (Goldenson and Crispino 2015), and has been linked to poor prognosis. Aurora B kinase inhibitors have been suggested as anti-cancer treatments (Yu et al. 2017).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the N-terminus of Human aurora-B kinase

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q96GD4
Entrez Gene
AURKB
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000780 condensed nuclear chromosome, centromeric region
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005819 spindle
GO:0006468 protein phosphorylation
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0032133 chromosome passenger complex
GO:0034501 protein localization to kinetochore
Regulatory
For research purposes only

Applications of Aurora-B Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/200 1/500
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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