ATP Citrate Lyase antibody | 5F8D11

Mouse anti ATP Citrate Lyase

Product Type
PrecisionAb Monoclonal
Clone
5F8D11
Isotype
IgG1
Format
Purified
Specificity
ATP Citrate Lyase

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VMA00116
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Mouse anti Human ATP citrate lyase antibody recognizes ATP citrate lyase, also known as ATP citrate synthase, ATP-citrate (pro-S-)-lyase, ATP-citrate synthase and citrate cleavage enzyme.

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for ATP citrate lyase (provided by RefSeq, Jul 2008).

Mouse anti Human ATP citrate lyase antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ATP citrate lyase detects a band of approximately 125 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human ATP Citrate Lysate, amino acids 306-502, expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P53396
Entrez Gene
ACLY
GO Terms
GO:0005515 protein binding
GO:0003878 ATP citrate synthase activity
GO:0005829 cytosol
GO:0004775 succinate-CoA ligase (ADP-forming) activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006101 citrate metabolic process
GO:0006112 energy reserve metabolic process
GO:0006200 ATP catabolic process
GO:0008815 citrate (pro-3S)-lyase activity
GO:0009346 citrate lyase complex
GO:0015936 coenzyme A metabolic process
GO:0019432 triglyceride biosynthetic process
GO:0031325 positive regulation of cellular metabolic process
GO:0035338 long-chain fatty-acyl-CoA biosynthetic process
GO:0044262 cellular carbohydrate metabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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