ARNT antibody | E01/1H8

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Mouse anti ARNT

Product Type
PrecisionAb Monoclonal
Clone
E01/1H8
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00963
Datasheet
IP WB
SDS
100 µl loader

Mouse anti ARNT antibody recognizes aryl hydrocarbon receptor nuclear translocator, also known as HIF1-beta, bHLHe2. ARNT is a transcription factor which predominantly binds HIF1-alpha or aryl hydrocarbon receptor, forming the heterodimer complex HIF1 which regulates expression of genes involved with a variety of physiological and pathological processes (Wu et al. 2018). Genes activated by HIF have a hypoxia-response element (HRE) within their promoter or enhancer. In this way, ARNT participates in the cellular response to reduced oxygen concentration (Mandl and Depping 2017). Under varying oxygen concentrations in cellular microenvironments, ARNT can also promote cell survival and angiogenesis (Shieh et al. 2014). In cancer, ARNT regulates tumorigenesis by activating genes involved with tumor growth and angiogenesis. Accordingly, ARNT expression has been identified in a range of cancer types and has been suggested as a prognostic biomarker and a target for cancer therapies (Huang et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti ARNT detects a band of approximately 94 kDa in MCF-7 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 469-789 of human ARNT
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P27540
Entrez Gene
ARNT
GO Terms
GO:0001666 response to hypoxia
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0046886 positive regulation of hormone biosynthetic process
GO:0017162 aryl hydrocarbon receptor binding
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045821 positive regulation of glycolysis
GO:0046982 protein heterodimerization activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ARNT antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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