Browsing Modes for Business Users

ARNT antibody | E01/1H8

ISO 9001 Quality Assurance Manufacturing | Print
siRNA Validated
IP-MS Validated
Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ARNT antibody (1/1000, VMA00963) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to ARNT (molecular weight 94 kDa)
Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of ARNT from MCF-7 whole cell lysate. The Input lane is 10% input; the ARNT-IP lane is the immunoprecipitation with the ARNT antibody (2.5 µg, VMA00963); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using ARNT antibody (1/1000, VMA00963) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to ARNT.
Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against ARNT (1 & 2) or a scrambled siRNA (-) probed with Mouse anti ARNT antibody (VMA00963) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 137 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti ARNT Antibody, clone E01/1H8 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of ARNT from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against ARNT (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti ARNT

Product Type
PrecisionAb Monoclonal
Clone
E01/1H8
Isotype
IgG1
Format
Purified
Specificity
ARNT

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00963
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
List Price Your Price
loader

Mouse anti ARNT antibody recognizes aryl hydrocarbon receptor nuclear translocator, also known as HIF1-beta, bHLHe2. ARNT is a transcription factor which predominantly binds HIF1-alpha or aryl hydrocarbon receptor, forming the heterodimer complex HIF1 which regulates expression of genes involved with a variety of physiological and pathological processes (Wu et al. 2018). Genes activated by HIF have a hypoxia-response element (HRE) within their promoter or enhancer. In this way, ARNT participates in the cellular response to reduced oxygen concentration (Mandl and Depping 2017). Under varying oxygen concentrations in cellular microenvironments, ARNT can also promote cell survival and angiogenesis (Shieh et al. 2014). In cancer, ARNT regulates tumorigenesis by activating genes involved with tumor growth and angiogenesis. Accordingly, ARNT expression has been identified in a range of cancer types and has been suggested as a prognostic biomarker and a target for cancer therapies (Huang et al. 2015). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti ARNT detects a band of approximately 94 kDa in MCF-7 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 469-789 of human ARNT
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P27540
Entrez Gene
ARNT
GO Terms
GO:0001666 response to hypoxia
GO:0001938 positive regulation of endothelial cell proliferation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0046886 positive regulation of hormone biosynthetic process
GO:0017162 aryl hydrocarbon receptor binding
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045821 positive regulation of glycolysis
GO:0046982 protein heterodimerization activity

VMA00963

100005852

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note