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ARHGDIA antibody | OTI1F2

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Western Blot Validated
Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with ARHGDIA antibody (VMA00179) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 18 second exposure. Arrow points to ARHGDIA (molecular weight 27 kDa).
Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of ARHGDIA knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti ARHGDIA antibody (VMA00179) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 54 second and (B) 30 second exposure.
Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of ARHGDIA from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti ARHGDIA

Product Type
PrecisionAb Monoclonal
Clone
OTI1F2
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00179
Datasheet
WB
SDS
100 µl loader

Mouse anti Human ARHGDIA antibody recognizes ARHGDIA, also known as GDP-dissociation inhibitor, aplysia RAS-related 1, epididymis secretory sperm binding protein Li 47e and rho GDP-dissociation inhibitor 1.

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).

Mouse anti Human ARHGDIA antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ARHGDIA detects a band of approximately 27 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ARHGDIA (NP_004300) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P52565
Entrez Gene
ARHGDIA
GO Terms
GO:0005829 cytosol
GO:0005094 Rho GDP-dissociation inhibitor activity
GO:0005096 GTPase activator activity
GO:0005856 cytoskeleton
GO:0006916 anti-apoptosis
GO:0006928 cellular component movement
GO:0007162 negative regulation of cell adhesion
GO:0007266 Rho protein signal transduction
GO:0042802 identical protein binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050771 negative regulation of axonogenesis
GO:0050772 positive regulation of axonogenesis
GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ARHGDIA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Product Specific References

Further Reading

  1. Leffers H et al. (1993) Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.
    Exp Cell Res. 209 (2): 165-74.

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