ARHGDIA antibody | OTI1F2

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Anti ARHGDIA Antibody, clone OTI1F2 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ARHGDIA antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 18 second exposure. Arrow points to ARHGDIA (molecular weight 27 kDa)

Mouse anti ARHGDIA

ARHGDIA antibody recognizes Rho GDP-dissociation inhibitor 1, also known as aplysia RAS-related 1 or epididymis secretory sperm binding protein Li 47e. This antibody has been extensively validated for western blotting using whole cell lysates.

ARHGDIA Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F2
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00179	WB	100 µl
VMA00179KT	WB	2 Westerns
With Lysate		400µg

Mouse anti Human ARHGDIA antibody recognizes ARHGDIA, also known as GDP-dissociation inhibitor, aplysia RAS-related 1, epididymis secretory sperm binding protein Li 47e and rho GDP-dissociation inhibitor 1.

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).

Mouse anti Human ARHGDIA antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti ARHGDIA detects a band of approximately 27 kDa in HEK293 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human ARHGDIA (NP_004300) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P52565 Related reagents
Entrez Gene: ARHGDIA Related reagents
GO Terms: GO:0005829 cytosol; GO:0005094 Rho GDP-dissociation inhibitor activity; GO:0005096 GTPase activator activity; GO:0005856 cytoskeleton; GO:0006916 anti-apoptosis; GO:0006928 cellular component movement; GO:0007162 negative regulation of cell adhesion; GO:0007266 Rho protein signal transduction; GO:0042802 identical protein binding; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050771 negative regulation of axonogenesis; GO:0050772 positive regulation of axonogenesis; GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of ARHGDIA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Product Specific References

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

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