ARHGDIA antibody | OTI1F2
Mouse anti ARHGDIA
ARHGDIA antibody recognizes Rho GDP-dissociation inhibitor 1, also known as aplysia RAS-related 1 or epididymis secretory sperm binding protein Li 47e. This antibody has been extensively validated for western blotting using whole cell lysates.
ARHGDIA Antibody with Control Lysate
ARHGDIA antibody recognizes Rho GDP-dissociation inhibitor 1, also known as aplysia RAS-related 1 or epididymis secretory sperm binding protein Li 47e. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1F2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00179 | WB | 100 µl | ||||
| VMA00179KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ARHGDIA antibody recognizes ARHGDIA, also known as GDP-dissociation inhibitor, aplysia RAS-related 1, epididymis secretory sperm binding protein Li 47e and rho GDP-dissociation inhibitor 1.
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).
Mouse anti Human ARHGDIA antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).
Mouse anti Human ARHGDIA antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ARHGDIA detects a band of approximately 27 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ARHGDIA (NP_004300) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P52565 Related reagents
- Entrez Gene
- ARHGDIA Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0005094 Rho GDP-dissociation inhibitor activity
- GO:0005096 GTPase activator activity
- GO:0005856 cytoskeleton
- GO:0006916 anti-apoptosis
- GO:0006928 cellular component movement
- GO:0007162 negative regulation of cell adhesion
- GO:0007266 Rho protein signal transduction
- GO:0042802 identical protein binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050771 negative regulation of axonogenesis
- GO:0050772 positive regulation of axonogenesis
- GO:0051056 regulation of small GTPase mediated signal transduction
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ARHGDIA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Product Specific References
Further Reading
-
Leffers H et al. (1993) Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.
Exp Cell Res. 209 (2): 165-74.
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