ARHGDIA antibody | OTI1F2
KO Validated
Mouse anti Human ARHGDIA antibody, clone OTI1F2 recognizes ARHGDIA, also known as GDP-dissociation inhibitor, aplysia RAS-related 1, epididymis secretory sperm binding protein Li 47e and rho GDP-dissociation inhibitor 1.
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).
Mouse anti Human ARHGDIA antibody, clone OTI1F2 detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al. 1993).
Mouse anti Human ARHGDIA antibody, clone OTI1F2 detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ARHGDIA detects a band of approximately 27 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ARHGDIA (NP_004300) produced in HEK293T cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P52565
- Entrez Gene
- ARHGDIA
- GO Terms
- GO:0005829 cytosol
- GO:0005094 Rho GDP-dissociation inhibitor activity
- GO:0005096 GTPase activator activity
- GO:0005856 cytoskeleton
- GO:0006916 anti-apoptosis
- GO:0006928 cellular component movement
- GO:0007162 negative regulation of cell adhesion
- GO:0007266 Rho protein signal transduction
- GO:0042802 identical protein binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050771 negative regulation of axonogenesis
- GO:0050772 positive regulation of axonogenesis
- GO:0051056 regulation of small GTPase mediated signal transduction
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ARHGDIA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Product Specific References
Further Reading
-
Leffers H et al. (1993) Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.
Exp Cell Res. 209 (2): 165-74.
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