Argonaute 2 antibody | H01/6G5

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Mouse anti Argonaute 2

Argonaute 2 antibody recognizes argonaute 2, also known as PAZ Piwi domain protein, PPD, eIF2C 2, eukaryotic translation initiation factor 2C, or protein slicer.

Argonaute 2 Antibody with Control Lysate

Argonaute 2 antibody recognizes argonaute 2, also known as PAZ Piwi domain protein, PPD, eIF2C 2, eukaryotic translation initiation factor 2C, or protein slicer.

Product Type
PrecisionAb™ Monoclonal
Clone
H01/6G5
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00681 WB 100 µl
VMA00681KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human argonaute 2 antibody recognizes argonaute 2, also known as PPD, eIF2C 2, eukaryotic translation initiation factor 2C, AGO2 and protein slicer.

AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).

Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti argonaute 2 detects a band of approximately 90 kDa in HeLa cell lysates
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-516 of human argonaute 2
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9UKV8 Related reagents
Entrez Gene
EIF2C2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000340 RNA 7-methylguanosine cap binding
GO:0000932 cytoplasmic mRNA processing body
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0005845 mRNA cap binding complex
GO:0006350 transcription
GO:0016442 RNA-induced silencing complex
GO:0031054 pre-microRNA processing
GO:0035068 micro-ribonucleoprotein complex
GO:0035197 siRNA binding
GO:0035278 negative regulation of translation involved in gene silencing by miRNA
GO:0035279 mRNA cleavage involved in gene silencing by miRNA
GO:0045449 regulation of transcription
GO:0045947 negative regulation of translational initiation
GO:0070551 endoribonuclease activity, cleaving siRNA-paired mRNA
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Argonaute 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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