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Argonaute 2 antibody | H01/6G5

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Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with argonaute 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to argonaute 2 (molecular weight 90 kDa)
Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of argonaute 2 from HeLa whole cell lysate. The Input lane is 10% input; the ARGONAUTE 2-IP lane is the immunoprecipitation with the argonaute 2 antibody (2.5 µg, VMA00681); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA929). Western blot analysis was performed using argonaute 2 antibody (1/1,000, VMA00681) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to argonaute 2.
Anti Argonaute 2 Antibody, clone H01/6G5 (PrecisionAb Monoclonal Antibody) gallery image 3
Figure A. FITC conjugated mouse anti human CD95 (MCA1539F) and Mouse anti mouse IgG2a isotype control (MCA929) detected with RPE conjugated goat anti mouse IgG2a (STAR133PE). Figure B. FITC conjugated mouse anti human CD95 (MCA1539F) and Mouse anti Argonaute 2 (VMA00681) detected with RPE conjugated goat anti mouse IgG2a (STAR133PE). All experiments performed on Leucoperm (BUF09) fixed and permeabilized HeLa cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Argonaute 2

Product Type
PrecisionAb Monoclonal
Clone
H01/6G5
Isotype
IgG2a
Format
Purified
Specificity
Argonaute 2

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VMA00681
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Mouse anti Human argonaute 2 antibody recognizes argonaute 2, also known as PPD, eIF2C 2, eukaryotic translation initiation factor 2C, AGO2 and protein slicer.

AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).

Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti argonaute 2 detects a band of approximately 90 kDa in HeLa cell lysates.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant protein of amino acids 1-516 of human argonaute 2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

References for Argonaute 2 antibody

  1. Ji, C. et al. (2020) Circ_0091581 Promotes the Progression of Hepatocellular Carcinoma Through Targeting miR-591/FOSL2 Axis.
    Dig Dis Sci. Oct 11 [Epub ahead of print].

UniProt
Q9UKV8
Entrez Gene
EIF2C2
GO Terms
GO:0005515 protein binding
GO:0000340 RNA 7-methylguanosine cap binding
GO:0000932 cytoplasmic mRNA processing body
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005634 nucleus
GO:0005845 mRNA cap binding complex
GO:0006350 transcription
GO:0016442 RNA-induced silencing complex
GO:0031054 pre-microRNA processing
GO:0035068 micro-ribonucleoprotein complex
GO:0035197 siRNA binding
GO:0035278 negative regulation of translation involved in gene silencing by miRNA
GO:0035279 mRNA cleavage involved in gene silencing by miRNA
GO:0045449 regulation of transcription
GO:0045947 negative regulation of translational initiation
GO:0070551 endoribonuclease activity, cleaving siRNA-paired mRNA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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