Argonaute 2 antibody | H01/6G5
Mouse anti Argonaute 2
Argonaute 2 antibody recognizes argonaute 2, also known as PAZ Piwi domain protein, PPD, eIF2C 2, eukaryotic translation initiation factor 2C, or protein slicer.
Argonaute 2 Antibody with Control Lysate
Argonaute 2 antibody recognizes argonaute 2, also known as PAZ Piwi domain protein, PPD, eIF2C 2, eukaryotic translation initiation factor 2C, or protein slicer.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- H01/6G5
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00681 | WB | 100 µl | ||||
| VMA00681KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human argonaute 2 antibody recognizes argonaute 2, also known as PPD, eIF2C 2, eukaryotic translation initiation factor 2C, AGO2 and protein slicer.
AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).
Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).
Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti argonaute 2 detects a band of approximately 90 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-516 of human argonaute 2
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9UKV8 Related reagents
- Entrez Gene
- EIF2C2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000340 RNA 7-methylguanosine cap binding
- GO:0000932 cytoplasmic mRNA processing body
- GO:0003743 translation initiation factor activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005634 nucleus
- GO:0005845 mRNA cap binding complex
- GO:0006350 transcription
- GO:0016442 RNA-induced silencing complex
- GO:0031054 pre-microRNA processing
- GO:0035068 micro-ribonucleoprotein complex
- GO:0035197 siRNA binding
- GO:0035278 negative regulation of translation involved in gene silencing by miRNA
- GO:0035279 mRNA cleavage involved in gene silencing by miRNA
- GO:0045449 regulation of transcription
- GO:0045947 negative regulation of translational initiation
- GO:0070551 endoribonuclease activity, cleaving siRNA-paired mRNA
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Argonaute 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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