Argonaute 2 antibody | H01/6G5
Mouse anti Argonaute 2
Argonaute 2 antibody recognizes argonaute 2, also known as PAZ Piwi domain protein, PPD, eIF2C 2, eukaryotic translation initiation factor 2C, or protein slicer.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- H01/6G5
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00681 | WB | 100 µl |
|
Mouse anti Human argonaute 2 antibody recognizes argonaute 2, also known as PPD, eIF2C 2, eukaryotic translation initiation factor 2C, AGO2 and protein slicer.
AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).
Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
AGO2 encodes a member of the argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for AGO2 (provided by RefSeq, Sep 2009).
Mouse anti Human argonaute 2 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti argonaute 2 detects a band of approximately 90 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-516 of human argonaute 2
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9UKV8 Related reagents
- Entrez Gene
- EIF2C2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000340 RNA 7-methylguanosine cap binding
- GO:0000932 cytoplasmic mRNA processing body
- GO:0003743 translation initiation factor activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005634 nucleus
- GO:0005845 mRNA cap binding complex
- GO:0006350 transcription
- GO:0016442 RNA-induced silencing complex
- GO:0031054 pre-microRNA processing
- GO:0035068 micro-ribonucleoprotein complex
- GO:0035197 siRNA binding
- GO:0035278 negative regulation of translation involved in gene silencing by miRNA
- GO:0035279 mRNA cleavage involved in gene silencing by miRNA
- GO:0045449 regulation of transcription
- GO:0045947 negative regulation of translational initiation
- GO:0070551 endoribonuclease activity, cleaving siRNA-paired mRNA
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Argonaute 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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