Androgen Receptor antibody | RM254

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Rabbit anti Androgen Receptor

Product Type
Monoclonal Antibody
Clone
RM254
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6308
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human androgen receptor antibody recognizes androgen receptor (AR), also known as Dihydrotestosterone receptor (DHTR), NR3C4. Androgen Receptor is a ligand-activated transcription factor that belongs to a large family of nuclear receptor proteins. Unbound Androgen Receptor resides predominately in the cytoplasm as a heteromeric complex with hsp90 and other chaperone proteins. Circulating testosterone is converted to dihydrotestosterone (DHT) which binds to androgen receptor in the cytoplasm, releasing androgen receptor from its inhibition with HSPs and allowing translocation to the nucleus, where androgen receptor can initiate transcription of its target genes (Tan et al. 2015). Androgen signaling plays a role in pathogenesis of prostate cancer, and gonadal depletion of androgens is a central therapy for advanced prostate cancer. However, progression to castration-resistant prostate cancer has led to development of therapies that directly target the androgen receptor (Dai et al. 2017) Androgen receptor also seems to play a major role in carcinogenesis of triple negative breast cancer (TNBC), a very aggressive cancer type which comprises 15 to 20% of all breast cancers (Gerratana et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the N-terminus of androgen receptor

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P10275
Entrez Gene
AR
GO Terms
GO:0016049 cell growth
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005102 receptor binding
GO:0004882 androgen receptor activity
GO:0005497 androgen binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006810 transport
GO:0007267 cell-cell signaling
GO:0008013 beta-catenin binding
GO:0008134 transcription factor binding
GO:0008219 cell death
GO:0008270 zinc ion binding
GO:0008283 cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0019899 enzyme binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0046983 protein dimerization activity
Regulatory
For research purposes only

Applications of Androgen Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/2500
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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