Androgen Receptor antibody | RM254

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Anti Androgen Receptor Antibody, clone RM254 thumbnail image 1

Anti Androgen Receptor Antibody, clone RM254 thumbnail image 2

Anti Androgen Receptor Antibody, clone RM254 thumbnail image 3

Anti Androgen Receptor Antibody, clone RM254 gallery image 1 — Western blot analysis of 22RV1 whole cell lysates probed with Rabbit anti Androgen Receptor (**MCA6308**). Arrows point to full length androgen receptor. Splice variants are also marked.

Anti Androgen Receptor Antibody, clone RM254 gallery image 2 — Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using Rabbit anti Androgen Receptor (**MCA6308**) at a 1/2500 dilution.

Anti Androgen Receptor Antibody, clone RM254 gallery image 3 — Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cells sections using Rabbit anti Androgen Receptor (**MCA6308**) at a 1/2500 dilution.

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Rabbit anti Androgen Receptor

Product Type: Monoclonal Antibody
Clone: RM254
Isotype: IgG
Specificity: Androgen Receptor

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Product Code Applications Pack Size List Price Your Price Qty

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MCA6308 Datasheet Datasheet SDS SDS	P WB	0.1 ml		Log in	Get Quote
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Rabbit anti Human androgen receptor antibody recognizes androgen receptor (AR), also known as Dihydrotestosterone receptor (DHTR), NR3C4. Androgen Receptor is a ligand-activated transcription factor that belongs to a large family of nuclear receptor proteins. Unbound Androgen Receptor resides predominately in the cytoplasm as a heteromeric complex with hsp90 and other chaperone proteins. Circulating testosterone is converted to dihydrotestosterone (DHT) which binds to androgen receptor in the cytoplasm, releasing androgen receptor from its inhibition with HSPs and allowing translocation to the nucleus, where androgen receptor can initiate transcription of its target genes (Tan et al. 2015). Androgen signaling plays a role in pathogenesis of prostate cancer, and gonadal depletion of androgens is a central therapy for advanced prostate cancer. However, progression to castration-resistant prostate cancer has led to development of therapies that directly target the androgen receptor (Dai et al. 2017) Androgen receptor also seems to play a major role in carcinogenesis of triple negative breast cancer (TNBC), a very aggressive cancer type which comprises 15 to 20% of all breast cancers (Gerratana et al. 2018).

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen: A peptide corresponding to the N-terminus of androgen receptor
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/1000	1/2500
Western Blotting		1/100	1/1000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt: P10275
Entrez Gene: AR
GO Terms: GO:0016049 cell growth; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005102 receptor binding; GO:0004882 androgen receptor activity; GO:0005497 androgen binding; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0006810 transport; GO:0007267 cell-cell signaling; View More GO Terms; GO:0008013 beta-catenin binding; GO:0008134 transcription factor binding; GO:0008219 cell death; GO:0008270 zinc ion binding; GO:0008283 cell proliferation; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010843 promoter binding; GO:0016563 transcription activator activity; GO:0019899 enzyme binding; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0046983 protein dimerization activity