Rabbit anti Human AMPK alpha 1 antibody recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as PRKAA1, AMPK1. AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit (alpha 1 and alpha 2), which is stabilized by non-catalytic regulatory beta- and gamma- subunits (Yan et al. 2018). AMPK responds to changes in intracellular adenine nucleotide levels, regulating cellular energy balance. AMPK is activated by an increase in AMP/ADP relative to ATP, and leads to an increase in rate of catabolic (ATP-generating) pathways and decreases rate of anabolic (ATP-utilizing) pathways (Carling et al. 2017). This results in maintenance of cellular energy homeostasis under conditions of hypoxia, low glucose levels, oxidative stress. Evidence suggests that AMPK acts as a tumor suppressor and has been suggested as a target for therapy, opposing tumor progression in several cancer types in part by regulating metabolism and inflammation (Li et al. 2015).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to the residues near the C-terminus of Human AMPK alpha 1
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
- 12 months from date of despatch
- Entrez Gene
- GO Terms
activation of MAPK activity
response to hypoxia
cAMP-dependent protein kinase activity
metal ion binding
fatty acid biosynthetic process
cholesterol biosynthetic process
cell cycle arrest
insulin receptor signaling pathway
positive regulation of cholesterol biosynthetic process
positive regulation of anti-apoptosis
negative regulation of glucosylceramide biosynthetic process
regulation of fatty acid oxidation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of AMPK Alpha 1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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