Rabbit anti Human AMPK alpha 1 antibody recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as PRKAA1, AMPK1. AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit (alpha 1 and alpha 2), which is stabilized by non-catalytic regulatory beta- and gamma- subunits (Yan et al. 2018). AMPK responds to changes in intracellular adenine nucleotide levels, regulating cellular energy balance. AMPK is activated by an increase in AMP/ADP relative to ATP, and leads to an increase in rate of catabolic (ATP-generating) pathways and decreases rate of anabolic (ATP-utilizing) pathways (Carling et al. 2017). This results in maintenance of cellular energy homeostasis under conditions of hypoxia, low glucose levels, oxidative stress. Evidence suggests that AMPK acts as a tumor suppressor and has been suggested as a target for therapy, opposing tumor progression in several cancer types in part by regulating metabolism and inflammation (Li et al. 2015).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/100	1/200
Western Blotting		1/100	1/200