AMPK Alpha 1 antibody | RM301
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WB P ResetRabbit anti AMPK Alpha 1
- Product Type
- Monoclonal Antibody
- Clone
- RM301
- Isotype
- IgG
- Specificity
- AMPK Alpha 1
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Antibody Quality and 
Unrivaled 
| Rabbit anti Human AMPK alpha 1 antibody recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as PRKAA1, AMPK1. AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit (alpha 1 and alpha 2), which is stabilized by non-catalytic regulatory beta- and gamma- subunits (Yan et al. 2018). AMPK responds to changes in intracellular adenine nucleotide levels, regulating cellular energy balance. AMPK is activated by an increase in AMP/ADP relative to ATP, and leads to an increase in rate of catabolic (ATP-generating) pathways and decreases rate of anabolic (ATP-utilizing) pathways (Carling et al. 2017). This results in maintenance of cellular energy homeostasis under conditions of hypoxia, low glucose levels, oxidative stress. Evidence suggests that AMPK acts as a tumor suppressor and has been suggested as a target for therapy, opposing tumor progression in several cancer types in part by regulating metabolism and inflammation (Li et al. 2015). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to the residues near the C-terminus of Human AMPK alpha 1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/100 | 1/200 |
| Western Blotting | |
1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- Q13131
- Entrez Gene
- PRKAA1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0001666 response to hypoxia
- GO:0005829 cytosol
- GO:0004691 cAMP-dependent protein kinase activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006633 fatty acid biosynthetic process
- GO:0006695 cholesterol biosynthetic process
- View More GO Terms
- GO:0007050 cell cycle arrest
- GO:0008286 insulin receptor signaling pathway
- GO:0045542 positive regulation of cholesterol biosynthetic process
- GO:0045768 positive regulation of anti-apoptosis
- GO:0046318 negative regulation of glucosylceramide biosynthetic process
- GO:0046320 regulation of fatty acid oxidation
View more products with AMPK specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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