AMPK Alpha 1 antibody | RM301

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Rabbit anti AMPK Alpha 1

Product Type
Monoclonal Antibody
Clone
RM301
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6327
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human AMPK alpha 1 antibody recognizes 5'-AMP-activated protein kinase catalytic subunit alpha-1, also known as PRKAA1, AMPK1. AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit (alpha 1 and alpha 2), which is stabilized by non-catalytic regulatory beta- and gamma- subunits (Yan et al. 2018). AMPK responds to changes in intracellular adenine nucleotide levels, regulating cellular energy balance. AMPK is activated by an increase in AMP/ADP relative to ATP, and leads to an increase in rate of catabolic (ATP-generating) pathways and decreases rate of anabolic (ATP-utilizing) pathways (Carling et al. 2017). This results in maintenance of cellular energy homeostasis under conditions of hypoxia, low glucose levels, oxidative stress. Evidence suggests that AMPK acts as a tumor suppressor and has been suggested as a target for therapy, opposing tumor progression in several cancer types in part by regulating metabolism and inflammation (Li et al. 2015).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the residues near the C-terminus of Human AMPK alpha 1

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q13131
Entrez Gene
PRKAA1
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0001666 response to hypoxia
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006633 fatty acid biosynthetic process
GO:0006695 cholesterol biosynthetic process
GO:0007050 cell cycle arrest
GO:0008286 insulin receptor signaling pathway
GO:0045542 positive regulation of cholesterol biosynthetic process
GO:0045768 positive regulation of anti-apoptosis
GO:0046318 negative regulation of glucosylceramide biosynthetic process
GO:0046320 regulation of fatty acid oxidation
Regulatory
For research purposes only

Applications of AMPK Alpha 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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