ALIX antibody | OTI1A4

100% Secure


Mouse anti ALIX

ALIX antibody recognizes programmed cell death 6-interacting protein, also known as ALG-2 interacting protein 1, ALG-2-interacting protein X, PDCD6-interacting protein, or apoptosis-linked gene 2-interacting protein X.

ALIX Antibody with Control Lysate

ALIX antibody recognizes programmed cell death 6-interacting protein, also known as ALG-2 interacting protein 1, ALG-2-interacting protein X, PDCD6-interacting protein, or apoptosis-linked gene 2-interacting protein X.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1A4
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00273 WB 100 µl
VMA00273KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human ALIX antibody recognizes the programmed cell death 6-interacting protein, also known as ALG-2 interacting protein 1, ALG-2-interacting protein X, PDCD6-interacting protein, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4 and programmed cell death 6-interacting protein.

The PDCD6IP (ALIX) gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of PDCD6IP binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. PDCD6IP product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of PDCD6IP product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for PDCD6IP. Related pseudogenes have been identified on chromosome 15 (provided by RefSeq, Jan 2012).

Mouse anti Human ALIX antibody detects a band of 96 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ALIX detects a band of approximately 96 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human ALIX (NP_037506) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8WUM4 Related reagents
Entrez Gene
PDCD6IP Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005815 microtubule organizing center
GO:0007049 cell cycle
GO:0015031 protein transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0048306 calcium-dependent protein binding
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ALIX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted