AKT1 antibody | OTI4C11

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Mouse anti AKT1

AKT1 antibody recognizes the Rac-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha or proto-oncogene c-Akt. This antibody has been extensively validated for western blotting using whole cell lysates.

AKT1 Antibody with Control Lysate

AKT1 antibody recognizes the Rac-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha or proto-oncogene c-Akt. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4C11
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00253 WB 100 µl
VMA00253KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human AKT1 antibody recognizes the RAC-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt and rac protein kinase alpha.

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1 (provided by RefSeq, Jul 2011).

Mouse anti Human AKT1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti AKT1 detects a band of approximately 60 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human AKT1 (NP_005154) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P31749 Related reagents
Entrez Gene
AKT1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000060 protein import into nucleus, translocation
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
GO:0005654 nucleoplasm
GO:0005978 glycogen biosynthetic process
GO:0006417 regulation of translation
GO:0006469 negative regulation of protein kinase activity
GO:0006809 nitric oxide biosynthetic process
GO:0006924 activation-induced cell death of T cells
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0009408 response to heat
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0010975 regulation of neuron projection development
GO:0016071 mRNA metabolic process
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0019899 enzyme binding
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0030235 nitric-oxide synthase regulator activity
GO:0030307 positive regulation of cell growth
GO:0031018 endocrine pancreas development
GO:0031295 T cell costimulation
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0031999 negative regulation of fatty acid beta-oxidation
GO:0034405 response to fluid shear stress
GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045600 positive regulation of fat cell differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046777 protein autophosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0070141 response to UV-A
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of AKT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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