AKT1 antibody | OTI4C11

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Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with AKT1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to AKT1 (molecular weight 60 kDa)

Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of AKT1 CRISPR knockout HeLa (AKT1 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti AKT1 antibody (**VMA00253**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 600 second and (B) 30 second exposure.

Anti AKT1 Antibody, clone OTI4C11 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of AKT1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti AKT1

Product Type: PrecisionAb Monoclonal
Clone: OTI4C11
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00253 Datasheet	WB SDS	100 µl	Log in		Get Quote

Mouse anti Human AKT1 antibody recognizes the RAC-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt and rac protein kinase alpha.

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1 (provided by RefSeq, Jul 2011).

Mouse anti Human AKT1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti AKT1 detects a band of approximately 60 kDa in Jurkat cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human AKT1 (NP_005154) produced in HEK293T cells

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P31749
Entrez Gene: AKT1
GO Terms: GO:0005886 plasma membrane; GO:0000060 protein import into nucleus, translocation; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding; GO:0005654 nucleoplasm; GO:0005978 glycogen biosynthetic process; GO:0006417 regulation of translation; GO:0006469 negative regulation of protein kinase activity; GO:0006809 nitric oxide biosynthetic process; GO:0006924 activation-induced cell death of T cells; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0008629 induction of apoptosis by intracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0042802 identical protein binding; GO:0009408 response to heat; GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport; GO:0010975 regulation of neuron projection development; GO:0016071 mRNA metabolic process; GO:0046326 positive regulation of glucose import; GO:0046889 positive regulation of lipid biosynthetic process; GO:0019899 enzyme binding; GO:0018105 peptidyl-serine phosphorylation; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation; GO:0030235 nitric-oxide synthase regulator activity; GO:0030307 positive regulation of cell growth; GO:0031018 endocrine pancreas development; GO:0031295 T cell costimulation; GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S; GO:0031999 negative regulation of fatty acid beta-oxidation; GO:0034405 response to fluid shear stress; GO:0043325 phosphatidylinositol-3,4-bisphosphate binding; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045600 positive regulation of fat cell differentiation; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0046777 protein autophosphorylation; GO:0048009 insulin-like growth factor receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0070141 response to UV-A; GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of AKT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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