Akt (pSer473) antibody | RM251

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Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of lysates from 293 cells either grown in medium with serum, serum starved, or insulin treated, and probed with Rabbit anti AKT (pSer473) (1/1000, **VMA00900**) or anti-AKT1. Arrow points to AKT phosphorylated at serine 473.

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Rabbit anti AKT (pSer473) (**VMA00900**) at a 1/400 dilution.

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) gallery image 3 — **Fig. 1. Western blot analysis of whole cell lysates probed with A, Rabbit anti Akt Antibody or B, Rabbit anti Akt (pSer473) Antibody (1/1,000, VMA00900)**. It is followed by detection with HRP conjugated Goat Anti-Rabbit (1/10,000, 170-5046). Membranes were treated with (+) and without (-) lambda protein phosphatase as indicated and visualized on the ChemiDoc MP Imaging System.

Anti Akt (pSer473) Antibody, clone RM251 (PrecisionAb Monoclonal Antibody) gallery image 4 — **Fig. 2. Total Protein Normalization.** Normalized signal intensity of Akt (pSer473) from PVD treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein

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Rabbit anti Akt (pSer473)

Product Type: PrecisionAb Monoclonal
Clone: RM251
Isotype: IgG
Format: Purified
Specificity: Akt (pSer473)

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Rabbit anti Human Akt (pSer473) antibody recognizes RAC-alpha serine/threonine-protein kinase, also known as Protein Kinase B alpha, proto-oncogene c-Akt, RAC, when phosphorylated at serine 473. The Akt family comprises three serine-threonine kinases, Akt1, Akt2, and Akt3, which play important roles in cell survival and proliferation (Barnett et al. 2005). The PI3K/Akt signaling pathway is activated by growth factors, which in turn activates Akt, and regulates cell survival during stress (Hemmings and Restuccia et al. 2012). Phosphorylation of threonine 308 and serine 473 are required for full activation of AKT1 (Jacinto et al. 2006).

Target Species: Human
Western Blotting: Rabbit anti AKT (pSer473) antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse Expected from Sequence

Rat Expected from Sequence

Bovine Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen: A phospho-peptide corresponding to Human Akt (pSer473)
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence
Bovine	Expected from Sequence

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/200	1/500
Western Blotting		1/1000	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt: P31749; P31751; Q9Y243
Entrez Gene: AKT1; AKT2; AKT3
GO Terms: GO:0005886 plasma membrane; GO:0000060 protein import into nucleus, translocation; GO:0005829 cytosol; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding; GO:0005654 nucleoplasm; GO:0005978 glycogen biosynthetic process; GO:0006417 regulation of translation; View More GO Terms; GO:0006469 negative regulation of protein kinase activity; GO:0006809 nitric oxide biosynthetic process; GO:0006924 activation-induced cell death of T cells; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0008629 induction of apoptosis by intracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0042802 identical protein binding; GO:0009408 response to heat; GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport; GO:0010975 regulation of neuron projection development; GO:0016071 mRNA metabolic process; GO:0046326 positive regulation of glucose import; GO:0046889 positive regulation of lipid biosynthetic process; GO:0019899 enzyme binding; GO:0018105 peptidyl-serine phosphorylation; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030168 platelet activation; GO:0030235 nitric-oxide synthase regulator activity; GO:0030307 positive regulation of cell growth; GO:0031018 endocrine pancreas development; GO:0031295 T cell costimulation; GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S; GO:0031999 negative regulation of fatty acid beta-oxidation; GO:0034405 response to fluid shear stress; GO:0043325 phosphatidylinositol-3,4-bisphosphate binding; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045600 positive regulation of fat cell differentiation; GO:0045725 positive regulation of glycogen biosynthetic process; GO:0046777 protein autophosphorylation; GO:0048009 insulin-like growth factor receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0070141 response to UV-A; GO:0090004 positive regulation of establishment of protein localization in plasma membrane; GO:0005515 protein binding; GO:0005634 nucleus; GO:0032000 positive regulation of fatty acid beta-oxidation; GO:0007165 signal transduction; GO:0005794 Golgi apparatus; GO:0006468 protein phosphorylation