Akt (pSer473) antibody | RM251

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Rabbit anti Akt (pSer473)

Product Type
PrecisionAb Monoclonal
Clone
RM251
Isotype
IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00900
Datasheet
P WB
SDS
100 µl loader

Rabbit anti Human Akt (pSer473) antibody recognizes RAC-alpha serine/threonine-protein kinase, also known as Protein Kinase B alpha, proto-oncogene c-Akt, RAC, when phosphorylated at serine 473. The Akt family comprises three serine-threonine kinases, Akt1, Akt2, and Akt3, which play important roles in cell survival and proliferation (Barnett et al. 2005). The PI3K/Akt signaling pathway is activated by growth factors, which in turn activates Akt, and regulates cell survival during stress (Hemmings and Restuccia et al. 2012). Phosphorylation of threonine 308 and serine 473 are required for full activation of AKT1 (Jacinto et al. 2006).

Product Details

Target Species
Human
Western Blotting
Rabbit anti AKT (pSer473) antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to Human Akt (pSer473)

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P31749
P31751
Q9Y243
Entrez Gene
AKT1
AKT2
AKT3
GO Terms
GO:0005886 plasma membrane
GO:0000060 protein import into nucleus, translocation
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
GO:0005654 nucleoplasm
GO:0005978 glycogen biosynthetic process
GO:0006417 regulation of translation
GO:0006469 negative regulation of protein kinase activity
GO:0006809 nitric oxide biosynthetic process
GO:0006924 activation-induced cell death of T cells
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042802 identical protein binding
GO:0009408 response to heat
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0010975 regulation of neuron projection development
GO:0016071 mRNA metabolic process
GO:0046326 positive regulation of glucose import
GO:0046889 positive regulation of lipid biosynthetic process
GO:0019899 enzyme binding
GO:0018105 peptidyl-serine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0030235 nitric-oxide synthase regulator activity
GO:0030307 positive regulation of cell growth
GO:0031018 endocrine pancreas development
GO:0031295 T cell costimulation
GO:0031659 positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0031999 negative regulation of fatty acid beta-oxidation
GO:0034405 response to fluid shear stress
GO:0043325 phosphatidylinositol-3,4-bisphosphate binding
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045600 positive regulation of fat cell differentiation
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046777 protein autophosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0070141 response to UV-A
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
GO:0005515 protein binding
GO:0005634 nucleus
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0007165 signal transduction
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
Regulatory
For research purposes only

Applications of Akt (pSer473) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/200 1/500
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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