Actin F Type antibody | NH3
Filter by Application:
WB ResetMouse anti Human F-Actin
- Product Type
- Monoclonal Antibody
- Clone
- NH3
- Isotype
- IgM
- Specificity
- Actin F Type
Antibody Quality and Performance Guarantee
| Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human monocytes and U937 cell line.
- Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1 / 10 | |
| Flow Cytometry | |
1 / 10 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Western Blotting | |
1/100 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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References for Actin F Type antibody
-
Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).
Biochem Soc Trans. 16: 163-4. -
Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.
J Pathol. 158 (4): 311-7. -
McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.
Am J Physiol Cell Physiol. 291: C860-8. -
Bhonagiri, P. et al. (2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.
Endocrinology. 152: 3373-84. -
Chen, X. et al. (2013) Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.
PLoS Negl Trop Dis. 7 (12): e2606. -
Fang, S.H. et al. (2019) Relationship of α2-Macroglobulin with Steroid-Induced Femoral Head Necrosis: A Chinese Population-Based Association Study in Southeast China.
Orthop Surg. 11 (3): 481-486. -
Grice, B.A. et al. (2019) Excess membrane cholesterol is an early contributing reversible aspect of skeletal muscle insulin resistance in C57BL/6NJ mice fed a Western-style high-fat diet.
Am J Physiol Endocrinol Metab. 317 (2): E362-E373.
- UniProt
- P60709
- P63261
- Entrez Gene
- ACTB
- ACTG1
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0006928 cellular component movement
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0034329 cell junction assembly
- View More GO Terms
- GO:0034332 adherens junction organization
View more products with ACTIN specificity
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