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Actin F Type antibody | NH3

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Anti Human F-Actin Antibody, clone NH3 gallery image 1
Western blot analysis of HeLa whole cell lysate probed with Mouse anti-human F-Actin (MCA358G)followed by detection with HRP conjugated Rabbit F(ab')2 anti Mouse IgG (Human Adsorbed) (1/2,000 STAR13B) and visualized on the ChemiDoc MP with 22 second exposure. Arrow points at F-Actin

Mouse anti Human F-Actin

Product Type
Monoclonal Antibody
Clone
NH3
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA358G
Datasheet
C E F WB
SDS
0.5 mg loader

MCA358GT
Datasheet
C F IF
SDS
50 µg loader

Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.

In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human monocytes and U937 cell line.
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P60709
P63261
Entrez Gene
ACTB
ACTG1
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
Regulatory
For research purposes only

Applications of Actin F Type antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 / 10
Flow Cytometry 1 / 10
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Actin F Type antibody

  1. Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).
    Biochem. Soc. Trans. 16: 163-164.
  2. Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.
    J Pathol. 158 (4): 311-7.
  3. McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.
    Am J Physiol Cell Physiol. 291: C860-8.
  4. Bhonagiri, P. et al. (2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.
    Endocrinology. 152: 3373-84.
  5. Fang, S.H. et al. (2019) Relationship of α2-Macroglobulin with Steroid-Induced Femoral Head Necrosis: A Chinese Population-Based Association Study in Southeast China.
    Orthop Surg. 11 (3): 481-486.
  6. Chen, X. et al. (2013) Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.
    PLoS Negl Trop Dis. 7 (12): e2606.

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