Actin F Type antibody | NH3

In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human monocytes and U937 cell line.
- Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P60709
- P63261
- Entrez Gene
- ACTB
- ACTG1
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0006928 cellular component movement
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- Regulatory
- For research purposes only
Applications of Actin F Type antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1 / 10 | ||
Flow Cytometry | 1 / 10 | ||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Western Blotting | 1/100 | 1/500 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
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|
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
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|
Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
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|
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgM Negative Control | MCA692 | F | 100 Tests |
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Product Specific References
References for Actin F Type antibody
-
Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).
Biochem. Soc. Trans. 16: 163-164. -
Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.
J Pathol. 158 (4): 311-7. -
McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.
Am J Physiol Cell Physiol. 291: C860-8. -
Bhonagiri, P. et al. (2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.
Endocrinology. 152: 3373-84. -
Fang, S.H. et al. (2019) Relationship of α2-Macroglobulin with Steroid-Induced Femoral Head Necrosis: A Chinese Population-Based Association Study in Southeast China.
Orthop Surg. 11 (3): 481-486. -
Chen, X. et al. (2013) Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.
PLoS Negl Trop Dis. 7 (12): e2606.
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