Actin F Type antibody | NH3

Mouse anti Human F-Actin

Product Type
Monoclonal Antibody
Clone
NH3
Isotype
IgM
Specificity
Actin F Type

Product Code Applications Pack Size List Price Your Price Qty
MCA358G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F WB 0.5 mg loader
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loader
MCA358GT
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SDS Safety Datasheet SDS
C F IF 50 µg loader
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loader
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Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.

In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human monocytes and U937 cell line.
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 / 10
Flow Cytometry 1 / 10
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader
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Description Mouse IgM Negative Control

References for Actin F Type antibody

  1. Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).
    Biochem. Soc. Trans. 16: 163-164.
  2. Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.
    J Pathol. 158 (4): 311-7.
  3. McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.
    Am J Physiol Cell Physiol. 291: C860-8.
  4. Bhonagiri, P. et al. (2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.
    Endocrinology. 152: 3373-84.
  5. Fang, S.H. et al. (2019) Relationship of α2-Macroglobulin with Steroid-Induced Femoral Head Necrosis: A Chinese Population-Based Association Study in Southeast China.
    Orthop Surg. 11 (3): 481-486.
  6. Chen, X. et al. (2013) Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.
    PLoS Negl Trop Dis. 7 (12): e2606.
  7. Grice, B.A. et al. (2019) Excess membrane cholesterol is an early contributing reversible aspect of skeletal muscle insulin resistance in C57BL/6NJ mice fed a Western-style high-fat diet.
    Am J Physiol Endocrinol Metab. 317 (2): E362-E373.

Immunofluorescence

Western Blotting

UniProt
P60709
P63261
Entrez Gene
ACTB
ACTG1
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0006928 cellular component movement
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
View more products with ACTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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