Actin F Type antibody | NH3
Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.
In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human monocytes and U937 cell line.
- Approx. Protein Concentrations
- IgM concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P60709
- P63261
- Entrez Gene
- ACTB
- ACTG1
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005524 ATP binding
- GO:0005856 cytoskeleton
- GO:0006928 cellular component movement
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- Regulatory
- For research purposes only
Applications of Actin F Type antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1 / 10 | ||
| Flow Cytometry | 1 / 10 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Western Blotting | 1/100 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
|
|
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control | MCA692 | F | 100 Tests |
|
Product Specific References
References for Actin F Type antibody
-
Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).
Biochem. Soc. Trans. 16: 163-164. -
Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.
J Pathol. 158 (4): 311-7. -
McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.
Am J Physiol Cell Physiol. 291: C860-8. -
Bhonagiri, P. et al. (2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.
Endocrinology. 152: 3373-84. -
Fang, S.H. et al. (2019) Relationship of α2-Macroglobulin with Steroid-Induced Femoral Head Necrosis: A Chinese Population-Based Association Study in Southeast China.
Orthop Surg. 11 (3): 481-486. -
Chen, X. et al. (2013) Molecular characterization of severin from Clonorchis sinensis excretory/secretory products and its potential anti-apoptotic role in hepatocarcinoma PLC cells.
PLoS Negl Trop Dis. 7 (12): e2606. -
Grice, B.A. et al. (2019) Excess membrane cholesterol is an early contributing reversible aspect of skeletal muscle insulin resistance in C57BL/6NJ mice fed a Western-style high-fat diet.
Am J Physiol Endocrinol Metab. 317 (2): E362-E373.
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