Actin Beta antibody | AbD12141

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Human anti Human Actin Beta:HRP

Human anti Human Actin Beta

Human anti Human Actin Beta:DyLight®800

Human anti Human Actin Beta:DyLight®680

Product Type
Monoclonal Antibody
Clone
AbD12141
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA147P
Datasheet
WB
MSDS
0.1 mg loader

HCA147
Datasheet
WB
MSDS
0.1 mg loader

HCA147D800
Datasheet
WB
MSDS
0.1 mg loader

HCA147D680
Datasheet
WB
MSDS
0.1 mg loader

Human anti human actin beta antibody, clone AbD12141 recognizes the 42 kDa product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling.

Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products.

Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Vertebrates Expected from Sequence
Invertebrates Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 800 - liquid.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 680 - liquid.
Preparation
StrepTactin affinity chromatography
Preparation
StrepTactin affinity chromatography
Preparation
StrepTactin affinity chromatography
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified human beta-actin
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P60709
Entrez Gene
ACTB
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

DyLight®is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Actin Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting
Western Blotting 1/500 1/5000
Western Blotting
Western Blotting 1/1000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting
HCA147P is suitable for use as a loading control
Western Blotting
HCA147 detects a band of approximately 42 kDa in HeLa cell lysates. HCA147 is suitable for use as a loading control.
Western Blotting
HCA147D800 is suitable for use as a loading control
Western Blotting
HCA147D680 is suitable for use as a loading control

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti c-Myc:Alk.Phos. MCA2200A C E P WB* 0.1 mg loader
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 F* 100 Tests/1ml loader
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 F* 100 Tests/1ml loader
Mouse anti c-Myc:Biotin MCA2200B C E P WB* 0.1 mg loader
Mouse anti c-Myc:FITC MCA2200F F* 0.1 mg loader
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-dHLX-MSx2 Negative Control HCA102 E 0.1 mg loader

Product Specific References

References for Actin Beta antibody

  1. Walsh, C.A. et al. (2020) The mevalonate precursor enzyme HMGCS1 is a novel marker and key mediator of cancer stem cell enrichment in luminal and basal models of breast cancer.
    PLoS One. 15 (7): e0236187.