Actin Beta antibody | AbD12141
Dylight 680 conjugated Human anti Human beta actin antibody, clone AbD12141 (HCA147D680) used as a loading control for western blotting.
Image caption:
High expression of HMGCS1 was a consistent feature in CSC enriched subpopulations of luminal and basal breast cancer subtypes and, the silencing of HMGCS1 gene expression reduced the levels of CSC activity. A. HMGCS1 gene expression measured by qPCR in MCF-7, T47D and MDA-MB-231 (MDA-231) cell lines cultured as adherent monolayer or 16 hour CSC-enriched (CSC-en) cultures. HMGCS1 transcript levels are relative to individual monolayer controls. Mean ±SEM from three biological replicates are shown. B. HMGCS1 protein expression in MCF-7, T47D and MDA-MB-231 cell lines cultured as adherent monolayers, 16 hours suspension cultures or 5-days mammosphere cultures. A representative western blot and associated densitometry are shown. C. Representative western blot and associated densitometry of MCF-7, T47D and MDA-MB-231 cell lines transfected with HMGCS1 siRNA confirming the knockdown of HMGCS1 protein expression, relative to the scrambled negative control. β-actin was used as a loading control.
From: Citation: Walsh CA, Akrap N, Garre E, Magnusson Y, Harrison H, Andersson D, et al. (2020)
The mevalonate precursor enzyme HMGCS1 is a novel marker and key mediator of cancer stem cell enrichment in luminal and basal models of breast cancer.
PLoS ONE 15(7): e0236187.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Human anti Human Actin Beta
- Product Type
- Monoclonal Antibody
- Clone
- AbD12141
- Isotype
- HuCAL Fab bivalent
- Specificity
- Actin Beta
Antibody Quality and Performance Guarantee
| Human anti human actin beta antibody, clone AbD12141 recognizes the 42 kDa product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling. Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Vertebrates Expected from Sequence Invertebrates Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- StrepTactin affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified human beta-actin
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- Western Blotting
- Human anti Human actin beta antibody, clone AbD12141 detects a band of approximately 42 kDa in HeLa cell lysates. Human anti Human actin beta antibody, clone AbD12141 is suitable for use as a loading control.
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References for Actin Beta antibody
-
Walsh, C.A. et al. (2020) The mevalonate precursor enzyme HMGCS1 is a novel marker and key mediator of cancer stem cell enrichment in luminal and basal models of breast cancer.
PLoS One. 15 (7): e0236187. -
Lederhofer, J. et al. (2018) Development of a Virosomal RSV Vaccine Containing 3D-PHAD® Adjuvant: Formulation, Composition, and Long-Term Stability.
Pharm Res. 35 (9): 172.
- Synonyms
- ACTB
- RRID
- AB_10612234
- UniProt
- P60709
- Entrez Gene
- ACTB
HCA147
153727
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