Actin Beta antibody | AbD12141
Human anti Human Actin Beta:HRP
Human anti Human Actin Beta
Human anti Human Actin Beta:DyLight®800
Human anti Human Actin Beta:DyLight®680
- Product Type
- Monoclonal Antibody
- Clone
- AbD12141
- Isotype
- HuCAL Fab bivalent
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| HCA147P | WB | 0.1 mg | ||||
| HCA147 | WB | 0.1 mg | ||||
| HCA147D800 | WB | 0.1 mg | ||||
| HCA147D680 | WB | 0.1 mg |
Human anti human actin beta antibody, clone AbD12141 recognizes the 42 kDa product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modelling.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products.
Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.
Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between the human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products.
Actin beta is a commonly used Western blotting loading control for cell and tissue lysates across many species due to its ubiquitous expression and evolutionary conservation rendering antibodies against this target cross reactive with protein preparations from many different organisms.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Vertebrates Expected from Sequence Invertebrates Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 800 - liquid.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to DyLight 680 - liquid.
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- StrepTactin affinity chromatography
- Preparation
- StrepTactin affinity chromatography
- Preparation
- StrepTactin affinity chromatography
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified human beta-actin
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- 1.0 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of reconstitution
- Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P60709 Related reagents
- Entrez Gene
- ACTB Related reagents
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
DyLight®is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Actin Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | |||
| Western Blotting | 1/100 | 1/1000 | |
| Western Blotting | |||
| Western Blotting | 1/1000 | 1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
- Western Blotting
- HCA147P is suitable for use as a loading control
- Western Blotting
- HCA147 detects a band of approximately 42 kDa in HeLa cell lysates. HCA147 is suitable for use as a loading control.
- Western Blotting
- HCA147D800 is suitable for use as a loading control
- Western Blotting
- HCA147D680 is suitable for use as a loading control
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti c-Myc:Alk.Phos. | MCA2200A | 0.1 mg | C E P WB* | ||
| Mouse anti c-Myc:Alexa Fluor® 488 | MCA2200A488 | 100 Tests/1ml | F* | ||
| Mouse anti c-Myc:Alexa Fluor® 647 | MCA2200A647 | 100 Tests/1ml | F* | ||
| Mouse anti c-Myc:Biotin | MCA2200B | 0.1 mg | C E P WB* | ||
| Mouse anti c-Myc:FITC | MCA2200F | 0.1 mg | F* | ||
| Mouse anti c-Myc:HRP | MCA2200P | 0.1 mg | C E P WB* | ||
| Mouse anti c-Myc:RPE | MCA2200PE | 100 Tests | WB | ||
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | 75 µl | E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-dHLX-MSx2 Negative Control | HCA102 | 0.1 mg | C E F P WB |
