MHC Class I Monomorphic antibody | CVS22
Mouse anti Horse MHC Class I Monomorphic:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CVS22
- Isotype
- IgG2a
- Specificity
- MHC Class I Monomorphic
Antibody Quality and Performance Guarantee
Mouse anti Horse MHC class I antibody, clone CVS22 (MCA1086GA) used for evaluation of MHC class I expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.
From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.
doi: 10.1371/journal.pone.0122954.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Figure B. RPE conjugated Mouse anti Horse CD11a/CD18 antibody, clone CVS9 (MCA1081PE) and FITC conjugated Mouse anti Horse MHC Class I antibody, clone CVS22 (MCA1086F). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum.
Data acquired on the ZE5 Cell Analyzer.
Mouse anti Equine MHC Class I antibody, clone CVS22 (MCA1086GA) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
From: Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecalallogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F Reset| Mouse anti Horse MHC Class I Monomorphic antibody, clone CVS22 recognizes monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. MHC class I is expressed by all nucleated cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region. |
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Equine leucocytes.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the X63.Ag 8.653 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature.
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Horse MHC Class II Monomorphic:FITC | MCA1085F | F | 0.1 mg |
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| Description | Mouse anti Horse MHC Class II Monomorphic:FITC | ||||||
| Mouse anti Horse MHC Class II Monomorphic | MCA1085GA | C F IP | 0.1 mg |
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| Description | Mouse anti Horse MHC Class II Monomorphic | ||||||
References for MHC Class I Monomorphic antibody
-
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62:101-143 -
Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131 (1-2): 1-8. -
Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
Cytotherapy. 13 (4): 419-30. -
Soboll Hussey, G. et al. (2014) Innate immune responses of airway epithelial cells to infection with equine herpesvirus-1.
Vet Microbiol. 170 (1-2): 28-38. -
Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954. -
Maumus M et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Rapacz-leonard, A. et al. (2018) Major histocompatibility complex class I in the horse (Equus caballus) placenta during pregnancy and parturition.
Placenta. 74: 36-46.
View The Latest Product References
-
Kamm, J.L. et al. (2021) Immune response to allogeneic equine mesenchymal stromal cells.
Stem Cell Res Ther. 12 (1): 570.
MCA1086PE
151673
INN0112R
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