CD8 antibody | CVS21

Mouse anti Horse CD8:RPE

Product Type
Monoclonal Antibody
Clone
CVS21
Isotype
IgG2a
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1080PE
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Mouse anti Horse CD8 antibody, clone CVS21 recognizes the equine homolog of human CD8. Equine CD8 is expressed by a subset of T lymphocytes.

In addition to the CVS21 clone, other CVS clones recognising equine MHC and cell surface antigens are available.

Target Species
Horse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Equine peripheral blood mononuclear cells.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Horse CD4:FITC MCA1078F F 0.1 mg loader
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Description Mouse anti Horse CD4:FITC

References for CD8 antibody

  1. Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
    Domest Anim Endocrinol. 29: 203-13.
  2. Krakowski, L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition
    J Vet Res. 61 (2): 217-21.
  3. Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
    J Vet Intern Med. 33 (2): 889-96.
  4. Hillmann, A. et al. (2019) A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.
    PLoS One. 14 (6): e0218949.
  5. Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
    Vet Immunol Immunopathol. 199: 22-31.
  6. Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
    Animals (Basel). 11(9):2726.
  7. Schauer, M. et al. (2018) Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
    Sci Rep. 8 (1): 12332.
View more products with CD8 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Horse Products