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CD11a/CD18 antibody | CVS9

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Anti Horse CD11a/CD18 Antibody, clone CVS9 thumbnail image 1 Anti Horse CD11a/CD18 Antibody, clone CVS9 thumbnail image 2 Anti Horse CD11a/CD18 Antibody, clone CVS9 thumbnail image 3 Anti Horse CD11a/CD18 Antibody, clone CVS9 thumbnail image 4
Anti Horse CD11a/CD18 Antibody, clone CVS9 gallery image 1
Staining of equine peripheral blood lymphocytes with Mouse anti Horse CD11a/CD18 (MCA1081GA), followed by Goat anti Mouse IgG:FITC (STAR117F).
Anti Horse CD11a/CD18 Antibody, clone CVS9 gallery image 2
Staining of equine peripheral blood lymphocytes with Mouse anti Horse CD11a/CD18:RPE (MCA1081PE).
Anti Horse CD11a/CD18 Antibody, clone CVS9 gallery image 3
Staining of equine peripheral blood lymphocytes with Mouse anti Horse CD11a/CD18:FITC (MCA1081F).
Anti Horse CD11a/CD18 Antibody, clone CVS9 gallery image 4
Figure A. FITC conjugated Mouse anti Horse MHC Class I Monomorphic (MCA1086F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Horse MHC Class I Monomorphic (MCA1086F) and RPE conjugated Mouse anti Horse CD11a/CD18 (MCA1081PE). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse seum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Horse CD11a/CD18:RPE

Product Type
Monoclonal Antibody
Clone
CVS9
Isotype
IgG1
Specificity
CD11a/CD18

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MCA1081PE
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Mouse anti Horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells.

In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.

Target Species
Horse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Equine leucocytes.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for CD11a/CD18 antibody

  1. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
    Vet Immunol Immunopathol. 42 (1): 3-60.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. Hammond, S.A. et al. (1999) Functional characterization of equine dendritic cells propagated ex vivo using recombinant human GM-CSF and recombinant equine IL-4.
    Vet Immunol Immunopathol. 71 (3-4): 197-214.
  4. McClure JT et al. (2001) Immunophenotypic classification of leukemia in 3 horses.
    J Vet Intern Med. 15 (2): 144-52.
  5. Laval, K. et al. (2015) Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells.
    J Virol. 89 (21): 10912-23.
  6. Schröck, C. et al. (2017) Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.
    Vet Med Sci. 3 (4): 239-251.
  7. Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
    J Vet Diagn Invest. 10406387221077969.

MCA1081PE

164689 INN0112R

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