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CD45 antibody | IH-1

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Anti Guinea Pig CD45 Antibody, clone IH-1 gallery image 1
Published customer image:
Mouse anti Guinea pig CD45 antibody, clone IH-1 (MCA1130) used for the evaluation of CD45 expression on cultured bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Validation of MSC properties. a BM-MSCs were harvested and cultured from guinea pigs’ bone marrow from healthy (sham-exposed) or CS-exposed individuals. b Cell characterization was determined by plastic adhesion, colony forming capacity, and morphology. c Purity was checked by flow cytometric analysis of CD45 expression in BM-MSCs and compared with whole blood. Fluorescence intensity of the no-antibody (left panel), the secondary antibody control (middle panel) and fluorescence intensity in the presence of the CD45 specific antibody (right panel) was measured. The lower panel shows gene expression analysis of positive (CD105, CD90, CD73) and negative (CD11b, CD31, CD34) MSC markers and β-Actin. M: molecular weight; LS: lung tissue lysate. d Adipogenic and oesteogenic differentiation. Cells were incubated for 3 weeks in either induction or normal growth medium before von-Kossa and Alizarin red staining for calcium deposits or oil red to identify lipidic vacuoles

From: Tura-Ceide O, Lobo B, Paul T, Puig-Pey R, Coll-Bonfill N, García-Lucio J, Smolders V, Blanco I, Barberà JA, Peinado VI.
Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
Respir Res. 2017 Mar 23;18(1):50.

Mouse anti Guinea Pig CD45:FITC

Mouse anti Guinea Pig CD45

Product Type
Monoclonal Antibody
Clone
IH-1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1130F
Datasheet
F
SDS
0.1 mg loader

MCA1130
Datasheet
C F IP
SDS
0.2 mg loader

Mouse anti Guinea Pig CD45 antibody, clone IH-1 reacts with CD45 on all leucocytes.

Mouse anti Guinea Pig CD45 antibody, clone IH-1 immunoprecipitates proteins of M.Wt. 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.

Product Details

Target Species
Guinea Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Guinea pig leukemic B lymphocyte L2C cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD45 antibody

  1. Hart, I.J. et al. (1992) Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen.
    Immunology. 77 (3): 377-84.
  2. Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
    Infect Immun. 78: 1670-81.
  3. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.
  4. Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
    Infect Immun. 79 (5): 1889-97.
  5. Tura-Ceide, O. et al. (2017) Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
    Respir Res. 18 (1): 50.
  6. Ishikawa, M. et al. (2017) Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae.
    J Tissue Eng Regen Med. 11 (6): 1766-78.

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