Etanercept antibody | AbD26183_hIgG1

Human anti Etanercept:HRP

Product Type
Monoclonal Antibody
Clone
AbD26183_hIgG1
Isotype
IgG1
Specificity
Etanercept

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HCA279P
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E 0.1 mg loader
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Human anti etanercept, clone AbD26183_hIgG1 is an antibody that specifically recognizes etanercept at the region of the fusion of p75 with the Fc portion of human IgG1. The antibody does not inhibit the binding of etanercept to its target tumor necrosis factor alpha (TNFα); it binds to both free etanercept and to etanercept bound to its target TNFα and can be used to measure total etanercept very specifically.

The antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug in patient samples. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD25939 (HCA276) in monovalent Fab format, as the capture antibody.

Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A).

View a summary of all anti-etanercept antibodies.

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Etanercept
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=14.8 nM by real time, label-free molecular interaction analysis on immobilized etanercept
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Enbrel is a registered trademark of Immunex Corporation.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection antibody in a bridging ELISA.
Protocol: PK bridging ELISA to measure total drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Etanercept HCA276 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Etanercept
Human anti Etanercept HCA279 E 0.1 mg loader
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loader
Description Human anti Etanercept

Synonyms
ENBREL®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Etanercept specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"