IgY antibody | 14A3

Mouse anti Duck IgY Light Chain

Product Type
Monoclonal Antibody
Clone
14A3
Isotype
IgG1
Specificity
IgY
Region
LIGHT CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA2480
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F WB 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.

Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479), reveals the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also reveals a high percentage of CD8+ /14A3+ cells in duck bursa (Kothlow et al. 2005). The recognition of B cells by 14A3 using chBAFF-Flag (B cell activating factor).

Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese.

Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified Pekin duck yolk IgY.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)

References for IgY antibody

  1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  2. Kothlow, S. et al. (2008) Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain.
    Avian Pathol. 37:323-8

Further Reading

  1. Schneider, K .et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
    Int Immunol. 16 (1): 139-48.

RRID
AB_609820
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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