IgY antibody | 14A3
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Mouse anti Duck IgY Light Chain
- Product Type
- Monoclonal Antibody
- Clone
- 14A3
- Isotype
- IgG1
- Specificity
- IgY
- Region
- LIGHT CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479), reveals the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also reveals a high percentage of CD8+ /14A3+ cells in duck bursa (Kothlow et al. 2005). The recognition of B cells by 14A3 using chBAFF-Flag (B cell activating factor). Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese. Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting. |
- Target Species
- Duck
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Goose - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified Pekin duck yolk IgY.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
References for IgY antibody
-
Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
Dev Comp Immunol. 29 (8): 733-48. -
Kothlow, S. et al. (2008) Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain.
Avian Pathol. 37:323-8
Further Reading
-
Schneider, K .et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
Int Immunol. 16 (1): 139-48.
- RRID
- AB_609820
MCA2480
149947
155440
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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