IgY antibody | 14A3

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Mouse anti Duck IgY Light Chain

Product Type
Monoclonal Antibody
Clone
14A3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2480 E F WB 0.25 mg
Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.

Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa (Kothlow et al. 2005). The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor).

Mouse anti Duck IgY Light Chain antibody, clone 14A3 recognizes IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies.

Mouse anti Duck IgY Light Chain antibody, clone 14A3 detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Product Details

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
Purified Pekin duck yolk IgY.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgY antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB

Product Specific References

References for IgY antibody

  1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  2. Kothlow, S. et al. (2008) Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain.
    Avian Pathol. 37:323-8

Further Reading

  1. Schneider, K .et al. (2004) Chicken BAFF--a highly conserved cytokine that mediates B cell survival.
    Int Immunol. 16 (1): 139-48.