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Mouse anti Duck CD4 antibody, clone Du CD4-2 (MCA2478) used along with Mouse anti Duck CD8&aplha; antibody, clone Du CD8-1 (MCA2479) for the demonstration of lymphocyte subsets in goose spleen sections by immunohistochemistry using formalin fixed, paraffin embedded tissue sections.
Global characteristics of goslings after infection with GPV and H9N2. (A) Body weight change of GPV- and H9N2-infected goslings from day 0 to 5. ** p≤0.01, *** p≤0.001; (B) the expression level of IL-1β and IL-6 in the spleen tissues of GPV- and H9N2-infected goslings. ** p≤0.01, *** p≤0.001; (C) histological changes in goose spleens infected with GPV and H9N2 at 5 dpi. The spleen tissues from (a) mock-, (b) GPV-, and (c) H9N2-infected goslings were stained with H &E, and diffuse hemorrhage was observed in either of virus-infected spleen sections, scale bar = 100 μm; and (D) Detection of (a) GPV and (b) H9N2 antigen, as well as (c,d) CD4- and (e,f) CD8α-positive cells by immunohistochemical analysis. The dark brown represents positive signs for viral antigen, CD4, or CD8α molecules, scale bar = 100 μm.
From: Chen S, Wang A, Sun L, Liu F, Wang M, Jia R, Zhu D, Liu M, Yang Q, Wu Y, Sun K, Chen X, Cheng A.
Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.
Int J Mol Sci. 2016 Dec 1;17(12). pii: E1990.