CD45R antibody | YKIX753.22.2
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|Rat anti Dog CD45R antibody, clone YKIX753.22.2 recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobbold et al. 1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13 (Cobbold et al. 1994).|
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1ml distilled water
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Canine thymocytes
- Fusion Partners
- Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD45R antibody
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens 43 (3): 137-54.
Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol.146: 303-11.
Giunchetti, R.C. et al. (2008) Antigenicity of a whole parasite vaccine as promising candidate against canine leishmaniasis.
Res Vet Sci. 85 (1): 106-12.
Pawlak, A., et al. (2014) Immunophenotypic characterization of canine malignant lymphoma: a retrospective study of cases diagnosed in Poland Lower Silesia, over the period 2011–2013.
Vet Comp Oncol. 10.1111/vco.12112.
Reis, A.B. et al. (2014) Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
Vet Immunol Immunopathol. 157 (3-4): 190-6.
Reis, A.B. et al. (2005) Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.
Vet Immunol Immunopathol. 103 (3-4): 173-85.
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