CD45R antibody | YKIX753.22.2

Rat anti Dog CD45R:RPE

Product Type
Monoclonal Antibody
Clone
YKIX753.22.2
Isotype
IgG2b
Specificity
CD45R

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MCA1043PE
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Rat anti Dog CD45R antibody, clone YKIX753.22.2 recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobbold et al. 1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13 (Cobbold et al. 1994).

Target Species
Dog
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Canine thymocytes
Fusion Partners
Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2b Negative Control:RPE MCA6006PE F 100 Tests loader
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Description Rat IgG2b Negative Control:RPE

References for CD45R antibody

  1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens 43 (3): 137-54.
  2. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
    Clin Exp Immunol.146: 303-11.
  3. Giunchetti, R.C. et al. (2008) Antigenicity of a whole parasite vaccine as promising candidate against canine leishmaniasis.
    Res Vet Sci. 85 (1): 106-12.
  4. Pawlak, A., et al. (2014) Immunophenotypic characterization of canine malignant lymphoma: a retrospective study of cases diagnosed in Poland Lower Silesia, over the period 2011–2013.
    Vet Comp Oncol. 10.1111/vco.12112.
  5. Reis, A.B. et al. (2014) Cellular immunophenotypic profile in the splenic compartment during canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 157 (3-4): 190-6.
  6. Reis, A.B. et al. (2005) Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.
    Vet Immunol Immunopathol. 103 (3-4): 173-85.

RRID
AB_322645
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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