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Rat IgG2b Negative Control antibody

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Rat IgG2b Negative Control Antibody thumbnail image 1 Rat IgG2b Negative Control Antibody thumbnail image 2 Rat IgG2b Negative Control Antibody thumbnail image 3
Rat IgG2b Negative Control Antibody gallery image 1
Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat IgG2b isotype control (MCA6006F). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Rat anti Human CD18 (MCA503F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Rat IgG2b Negative Control Antibody gallery image 2
Figure A. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat IgG2b isotype control (MCA6006PE). Figure B. A647 conjugated Mouse anti Human CD4 (MCA1267A647) and RPE conjugated Rat anti Human CD28 (MCA709PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Rat IgG2b Negative Control Antibody gallery image 3
Figure A. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control (MCA6006F). Figure B. Purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649 and Rat anti Canine CD4 FITC (MCA1038F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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Rat IgG2b Negative Control:RPE

Product Type
Negative/Isotype Control
Isotype
IgG2b
Specificity
Rat IgG2b Negative Control

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MCA6006PE
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Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.

Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.		 Isotype controls savings up to 65%

Target Species
Negative Control
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
KLH
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Rat IgG2b Negative Control antibody

  1. Stavre, Z. et al. (2022) A role for neutrophils in early enthesitis in spondyloarthritis.
    Arthritis Res Ther. 24 (1): 24.

MCA6006PE

INN0615

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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