FOXP3 antibody | AbD07627

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Human anti Bovine FOXP3:Alexa Fluor® 647

Human anti Bovine FOXP3

Product Type
Monoclonal Antibody
Clone
AbD07627
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA116A647 F* 100 Tests/1ml
HCA116A E F* 50 µg
HCA116 E F* 0.1 mg
Human anti Bovine FoxP3 antibody, clone AbD07627 recognizes bovine FoxP3, a ~48 kDa forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis.

FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid conjugated to Alexa Fluor® 647.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Pack Size: 50 µg
Reconstitute with 0.5 ml distilled water
Pack Size: 0.1 mg
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Bovine Foxp3-N1 fusion protein
Approx. Protein Concentrations
Total protein concentration 0.05 mg/ml
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 50 µg
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Pack Size: 0.1 mg
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q2LEZ0 Related reagents
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents.

His-tag is a registered trademark of EMD Biosciences.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of FOXP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
ELISA
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of 0.1% Triton X-100 for this purpose.
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of 0.1% Triton X-100 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
ELISA
This product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 100 Tests/1ml F
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 1 ml F
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti Histidine Tag:FITC MCA1396F 0.1 mg F IF

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647 HCA052A647 1 ml E F
HuCAL Fab-dHLX-MH Negative Control HCA052A 50 µg C E P WB
HuCAL Fab-dHLX-MH Negative Control HCA052 0.1 mg C E F P WB

Application Based External Images

Flow Cytometry

Product Specific References

References for FOXP3 antibody

  1. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15 (1): 11-20.
  2. Walsh, N.M. et al. (2016) A Morphological and Immunophenotypic Map of the Immune Response in Merkel Cell Carcinoma.
    Hum Pathol. Mar 2. pii: S0046-8177(16)00064-2. [Epub ahead of print]
  3. Maślanka T & Jaroszewski JJ (2013) Foxp3 expression in bovine CD8+ T cells is associated with the intensity of CD25 expression.
    J Vet Med Sci. 75 (2): 241-4.
  4. Maślanka T & Jaroszewski JJ (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
    J Vet Sci. 14 (2): 125-34.
  5. Walsh, N.M. et al. (2016) A morphological and immunophenotypic map of the immune response in Merkel cell carcinoma.
    Hum Pathol. 52: 190-6.