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FOXP3 antibody | AbD07627

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Anti Bovine FOXP3 Antibody, clone AbD07627 thumbnail image 1
Anti Bovine FOXP3 Antibody, clone AbD07627 gallery image 1
Published customer image:
Horseradish peroxidase conjugated Mouse anti Bovine IgG2 antibody, clone IL-A73 (MCA2441P) used to evaluate bovine IgG2 levels in serum samples by ELISA.
Image caption:
Antibody profile of S19 and RB51 prime vaccinated, and RB51 revaccinated cattle measured by I-ELISA using S19 and RB51 whole-cell antigens.
The results are expressed as mean. Data for total IgG, IgG1 and IgG2 are shown. Vaccinations were indicated by arrows. Lowercase letters indicate statistical difference between days on same group (one-way ANOVA followed by paired t-test).

From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, et al. (2015)
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.
doi: 10.1371/journal.pone.0136696
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Human anti Bovine FOXP3:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
AbD07627
Isotype
HuCAL Fab bivalent
Specificity
FOXP3

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Product Code Applications Pack Size List Price Your Price Qty
HCA116A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/1ml
List Price Your Price

Human anti Bovine FoxP3 antibody, clone AbD07627 recognizes bovine FoxP3, a ~48 kDa forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis.

FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid conjugated to Alexa Fluor 647.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Bovine Foxp3-N1 fusion protein
Approx. Protein Concentrations
Total protein concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of 0.1% Triton X-100 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

References for FOXP3 antibody

  1. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15 (1): 11-20.
  2. Maślanka T & Jaroszewski JJ (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
    J Vet Sci. 14 (2): 125-34.
  3. Maślanka T & Jaroszewski JJ (2013) Foxp3 expression in bovine CD8+ T cells is associated with the intensity of CD25 expression.
    J Vet Med Sci. 75 (2): 241-4.
  4. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus. S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.

Flow Cytometry

RRID
AB_1658062
UniProt
Q2LEZ0
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.

HCA116A647

1711

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