FOXP3 antibody | AbD07627

Human anti Bovine FOXP3

Product Type
Monoclonal Antibody
Clone
AbD07627
Isotype
HuCAL Fab bivalent
Specificity
FOXP3

Product Code Applications Pack Size List Price Your Price Qty
HCA116
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SDS Safety Datasheet SDS
E F * 0.1 mg loader
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Human anti Bovine FoxP3 antibody, clone AbD07627 recognizes bovine FoxP3, a ~48 kDa forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis.

FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Bovine Foxp3-N1 fusion protein
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of 0.1% Triton X-100 for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
ELISA
This product is suitable for use in direct ELISA applications

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Description Mouse anti Histidine Tag:Alexa Fluor® 647
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
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Description Mouse anti Histidine Tag:Biotin
Mouse anti Histidine Tag:FITC MCA1396F F IF 0.1 mg loader
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Description Product Code Applications Pack Size List Price Your Price Quantity
HuCAL Fab-dHLX-MH Negative Control HCA052 E 0.1 mg loader
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Description HuCAL Fab-dHLX-MH Negative Control

References for FOXP3 antibody

  1. Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
    Pol J Vet Sci. 15 (1): 11-20.
  2. Walsh, N.M. et al. (2016) A Morphological and Immunophenotypic Map of the Immune Response in Merkel Cell Carcinoma.
    Hum Pathol. Mar 2. pii: S0046-8177(16)00064-2. [Epub ahead of print]
  3. Maślanka T & Jaroszewski JJ (2013) Foxp3 expression in bovine CD8+ T cells is associated with the intensity of CD25 expression.
    J Vet Med Sci. 75 (2): 241-4.
  4. Maślanka T & Jaroszewski JJ (2013) In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25-CD4+ cells.
    J Vet Sci. 14 (2): 125-34.
  5. Walsh, N.M. et al. (2016) A morphological and immunophenotypic map of the immune response in Merkel cell carcinoma.
    Hum Pathol. 52: 190-6.
  6. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus. S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.

Flow Cytometry

RRID
AB_1658061
UniProt
Q2LEZ0
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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