CD282 antibody | AbD12542
Fitc conjugated Human anti Bovine CD282 antibody, clone AbD12542 (HCA152F) used to evaluate TLR2 expression on bovine PBMC by immunofluorescence.
Image caption:
Higher abundance of TLR2 and MHC class II in PBMC of MAP-resistant compared with persistently MAP-infected cows. (A1) Higher abundance of TLR2 in PBMC from MAP-resistant compared with persistently MAP-infected cows as measured by flow cytometry analysis with representative histograms. (A2) Higher mean fluorescence intensity of TLR2 positive PBMC from MAP-resistant compared to persistently MAP-infected cows. Mean fluorescence intensity from flow cytometry ± SD
From:Korbonits L, Kleinwort KJH, Amann B, Didier A, Märtlbauer E, Hauck SM, Deeg CA.
Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived LymphocyteProteome.
Metabolites. 2022 Sep 29;12(10):924.
doi: 10.3390/metabo12100924.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Human anti Bovine CD282:FITC
- Product Type
- Monoclonal Antibody
- Clone
- AbD12542
- Isotype
- HuCAL Fab bivalent
- Specificity
- CD282
Antibody Quality and Performance Guarantee
| Human anti Bovine CD282 antibody, clone AbD12542 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to fluorescein isothiocyanate (FITC) - liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Fc-fusion protein containing the sequence 21-588 from bovine TLR2
- Approx. Protein Concentrations
- Ig concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD282 antibody
-
Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139: 313-8. -
Garza-Cuartero, L. et al. (2016) Fasciola hepatica infection reduces Mycobacterium bovis burden and mycobacterial uptake and suppresses the pro-inflammatory response.
Parasite Immunol. 38 (7): 387-402. -
Conejeros, I. et al. (2015) Effect of the synthetic Toll-like receptor ligands LPS, Pam3CSK4, HKLM and FSL-1 in the function of bovine polymorphonuclear neutrophils.
Dev Comp Immunol. 52 (2): 215-25. -
Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924.
- Synonyms
- TLR2
- RRID
- AB_10846933
- UniProt
- Q95LA9
- Entrez Gene
- TLR2
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0006954 inflammatory response
- GO:0045087 innate immune response
HCA151F
0215
169113
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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