CD28 antibody | CC220
Mouse anti Bovine CD28:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- CC220
- Isotype
- IgG1
- Specificity
- CD28
Antibody Quality and Performance Guarantee
Mouse anti Bovine CD28 antibody, clone CC220 (MCA5778EL) used in vitro to evaluate the effect on interferon γ production by peripheral blood derived mononuclear cells from healthy and mycoplasmosis affected cattle
Image caption:
IFN‐γ production in bovine mycoplasmosis. IFN‐γ production was determined in supernatant of the PBMCs culture from mycoplasmosis‐afflicted and healthy control Holstein cattle by ELISA assay. PBMCs were incubated with the combination of anti‐bovine CD3 and anti‐bovine CD28 antibodies. Each line indicates the median production in each group.
From: Goto S, Konnai S, Okagawa T, Nishimori A, Maekawa N, Gondaira S, Higuchi H, Koiwa M, Tajima M, Kohara J, Ogasawara S, Kato Y, Suzuki Y, Murata S, Ohashi K.
Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immun Inflamm Dis. 2017 Sep;5(3):355-63.
doi: 10.1002/iid3.173.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD28 antibody, clone CC220 (MCA5778EL) used for the in vitro stimulation of bovine PBMCs for the subsequent evaluation of interferon γ production.
Image caption:
Activation of T-cell proliferation and interferon-γ (IFN-γ) production by Boch4G12.
(A and B) The proliferation of CD4+ and CD8+ cells in purified bovine CD3+ T cells. Peripheral blood mononuclear cells (PBMCs) isolated from healthy cattle were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), stimulated with 1 μg/ml anti-CD3 and anti-CD28 antibodies, and then cultured for 3 days with enhanced green fluorescent protein (EGFP) cells or programmed death-ligand 1 (PD-L1)/EGFP cells without antibodies (A), or PD-L1/EGFP cells in the presence of 10 μg/ml bovine IgG control or Boch4G12 (B). n = 10 and 6, respectively; A P-value less than 0.05 was considered statistically significant. *, P <0.05, paired t-test. (C) IFN-γ production in the culture supernatant of PBMCs from bovine leukemia virus (BLV)-infected cattle (n = 10, AL:5, PL:5). PBMCs were stimulated with the supernatant of FLK-BLV cells and cultured for 6 days in the presence of 10 μg/ml rat IgG control, 4G12, bovine IgG control or Boch4G12. The concentration of IFN-γ in the culture supernatants was measured using ELISA. A P-value less than 0.05 was considered statistically significant. **, P <0.01, Wilcoxon signed rank test. Data are the means of three replicate experiment
From: Nishimori A, Konnai S, Okagawa T, Maekawa N, Ikebuchi R, Goto S, et al. (2017)
In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
PLoS ONE 12(4): e0174916.
doi: 10.1371/journal.pone.0174916.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
FN Reset| Mouse anti Bovine CD28, clone CC220 recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 is able to stimulate bovine T lymphocytes in combination with anti-bovine CD3 (Nishimori et al. 2017). For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014). |
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Plasmid DNA encoding the bovine CD28 gene.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the SP2/0 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for CD28 antibody
-
Goto, S. et al. (2017) Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis.
Immun Inflamm Dis. 5 (3): 355-363. -
Nishimori, A. et al. (2017) In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.
PLoS One. 12 (4): e0174916. -
Cunha, P. et al. (2019) Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Sci Rep. 9 (1): 16115.
Further Reading
-
Hogg, A.E. et al. (2011) Characterization of age-related changes in bovine CD8+ T-cells.
Vet Immunol Immunopathol. 140 (1-2): 47-54.
- UniProt
- Q28071
- Entrez Gene
- CD28
- GO Terms
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0042102 positive regulation of T cell proliferation
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045840 positive regulation of mitosis
MCA5778A488
152560
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