CD205 antibody | IL-A114
Filter by Application:
F ResetMouse anti Bovine CD205
- Product Type
- Monoclonal Antibody
- Clone
- IL-A114
- Isotype
- IgG1
- Specificity
- CD205
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD205 antibody, clone IL-A114 recognizes bovine CD205 (also known as DEC205) and originally described as BoWC6. CD205 is a cell surface antigen with an approximate molecular weight of ~210-220 kDa. CD205 is expressed on afferent lymph dendritic cells (veiled cells), B cells, a subset of T cells and by cortical and medullary thymocytes. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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| Description | Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) | ||||||
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| Description | Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
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| Description | Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | ||||||
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Bovine Dendritic Cell Growth Kit | PBP014KZZ | FN | 1 ml | Log in | |||
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| Bovine Dendritic Cell Growth Kit | PBP015KZZ | FN | 5 ml | Log in | |||
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| Description | Bovine Dendritic Cell Growth Kit | ||||||
References for CD205 antibody
-
Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6.
Vet Immunol Immunopathol. 39 (1-3): 193-9. -
Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.
Vet Immunol Immunopathol. 39 (1-3): 283-90. -
Parsons, K.R. et al. (1993) Studies of monoclonal antibodies identifying two novel bovine lymphocyte antigen differentiation clusters: workshop clusters (WC) 6 and 7.
Vet Immunol Immunopathol. 39 (1-3): 187-92. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.
Immunology. 111 (3): 262-72. -
Tamao, H. et al. (2011) Distribution of immune cells and expression of interleukin receptors in ileal Peyer's patches of calves.
Cell Tissue Res. 346: 245-54. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365.
- Synonyms
- WC6 Antigen
- UniProt
- Q6WY07
MCA2450GA
1111
1111R
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