CD2 antibody | CC42
Mouse anti Bovine CD2 antibody, clone CC42 (MCA833GA) used for the evaluation of CD2 expression by bovine lymphocyte populations by flow cytometry.
Image caption:
CD2− natural killer (NK) cells are the predominant subset of NK cells present within skin‐draining afferent lymph (AL). Lymphocytes derived from peripheral blood (PB), AL and the lymph nodes (LNs) of seven calves were labeled with monoclonal antibodies to NKp46, CD3 and CD2 and analysed by flow cytometry. FACS plots from one representative animal illustrate the expression of NKp46 and CD2 by PB‐, AL‐ and LN‐derived lymphocytes (b). Gates were set based on Fluorescence Minus One controls.
From: Hamilton CA, Mahan S, Bell CR, Villarreal-Ramos B, Charleston B, Entrican G, Hope JC.
Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 2017 May;151(1):89-97.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD2 antibody, clone CC42 (MCA833GA) used for the evaluation of CD2 expression by bovine lymphocyte populations by flow cytometry.
Image caption:
CD2+ natural killer (NK) cells are the predominant subset of NK cells present in bovine skin. Pieces of skin were removed from calves at post mortem and enzymatically digested to obtain lymphocytes. Lymphocytes were labeled with monoclonal antibodies to NKp46 and CD2 and analysed by flow cytometry. FACS plots from three calves (303019, 403020 and 603022) illustrate the expression of NKp46 and CD2 by skin‐derived lymphocytes. Quadrants were set based on Fluorescence Minus One controls.
From: Hamilton CA, Mahan S, Bell CR, Villarreal-Ramos B, Charleston B, Entrican G, Hope JC.
Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 2017 May;151(1):89-97.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD2, clone CC42, recognizes the bovine homologue of human CD2. Clone CC42 inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/10 | 1/20 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
EDTA pH8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD2 antibody
-
Davis, W.C. & Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2).
Vet Immunol Immunopathol. 27: 43-50. -
Sopp, P. et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence.
Vet Immunol Immunopathol. 27 (1-3): 163-8. -
Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
Vet Immunol Immunopathol. 27 (1-3): 215-22. -
Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. -
Fikri, Y. et al. (2000) Purification and characterisation of bovine WC1+ gammadelta T lymphocytes from peripheral blood.
Vet Res. 31: 229-39. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Wilson, E. et al. (1999) A circulating bovine gamma delta T cell subset, which is found in large numbers in the spleen, accumulates inefficiently in an artificial site of inflammation: correlation with lack of expression of E-selectin ligands and L-selectin.
J Immunol.162: 4914-9. -
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. -
Grell, S.N. et al. (2005) Age-dependent differences in cytokine and antibody responses after experimental RSV infection in a bovine model.
Vaccine 23: 3412-23. -
Faldyna, M. et al. (1999) Leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats from herd infected with Mycobacterium avium subspecies paratuberculosis.
Vet. Med. Czech 44: 259-62 -
Faldyna, M. et al. (2006) γδ-TCR+ CD2– lymphocytes are recruited into bovine mammary gland after stimulation.
Veterinarni Medicina, 51: 258–64. -
Slama, P. et al. (2009) Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland lymphocytes.
Res Vet Sci. 87: 233-8. -
Bednarek, D. et al. (2003) Effect of steroidal and non-steroidal anti-inflammatory drugs in combination with long-acting oxytetracycline on non-specific immunity of calves suffering from enzootic bronchopneumonia.
Vet Microbiol. 96: 53-67. -
Hamilton, C.A. et al. (2017) Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 151 (1): 89-97.
