CD172a antibody | CC149



















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F IF C ResetMouse anti Bovine CD172a:RPE-Cy5
- Product Type
- Monoclonal Antibody
- Clone
- CC149
- Isotype
- IgG2b
- Specificity
- CD172a
Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin. |
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Bovine afferent veiled dendritic cells
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE-Cy5 488nm laser 496 667 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- Cy and CyDye are registered trademarks of GE Healthcare
The Lightning Link® technology used in the creation of this product is provided under an intellectual property license from Abcam Limited. For information on purchasing a license to this technology for purposes other than research contact Abcam at partnership@abcam.com. Lightning Link® is a registered trademark of Abcam Limited.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:RPE-Cy5 | MCA691C | F | 100 Tests |
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List Price | Your Price | ||||||
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Description | Mouse IgG2b Negative Control:RPE-Cy5 |
References for CD172a antibody
-
Smith, R. et al. (2003) A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes.
Blood.102: 2532-40. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
Immunology. 126:201-8 -
Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.
PLoS One. 4: e6414. -
Jensen, K. et al. (2014) Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.
Vet Immunol Immunopathol. 158 : 224-32. -
Hussen J et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
Dev Comp Immunol. 47 (2): 169-77. -
Eger, M. et al. (2015) Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets.
Vet Immunol Immunopathol. 166 (1-2): 33-42. -
Vachiery N et al. (2015) An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 46 (1): 117.
View The Latest Product References
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Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9. -
Pridans, C. et al. (2016) A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 197 (6): 2297-305. -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis in Canchim calves.
Vet Immunol Immunopathol. 230: 110132. -
Barut, G.T. et al. (2020) Transcriptomic profiling of bovine blood dendritic cells and monocytes following TLR stimulation.
Eur J Immunol. 50 (11): 1691-711. -
Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
Vet Immunol Immunopathol. 227: 110090. -
Tahoun, A. et al. (2020) Inflammasome Activation in Bovine Peripheral Blood-Derived Macrophages Is Associated with Actin Rearrangement.
Animals (Basel). 10 (4): 655. -
Ibeagha-Awemu, E.M. et al. (2021) Regionally Distinct Immune and Metabolic Transcriptional Responses in the Bovine Small Intestine and Draining Lymph Nodes During a Subclinical Mycobacterium avium subsp. paratuberculosis Infection.
Front Immunol. 12: 760931. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
Marzo, S. et al. (2022) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression.
Vet Immunol Immunopathol. 243: 110363. -
Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
Vet Immunol Immunopathol. 248: 110417. -
Cunha, P. et al. (2022) Simplified Approaches for the Production of Monocyte-Derived Dendritic Cells and Study of Antigen Presentation in Bovine.
Front Vet Sci. 9: 891893. -
Hong, S. et al. (2024) Impact of an Injectable Trace Mineral Supplement on the Immune Response and Outcome of Mannheimia haemolytica Infection in Feedlot Cattle.
Biol Trace Elem Res. Jun 10 [Epub ahead of print]. -
Scatà, C.M. et al. (2024) Characterization of Cellular Immune System at Different Ages in Water Buffalo (Bubalus bubalis)
Journal of Buffalo Science. 13: 133-139. -
Muir, A. et al. (2024) Single-cell analysis reveals lasting immunological consequences of influenza infection and respiratory immunization in the pig lung.
PLoS Pathog. 20 (7): e1011910. -
Gilbert, F.B. et al. (2025) Expression of FcμR by bovine mononuclear blood leukocytes.
Dev Comp Immunol. 162: 105304.
Further Reading
-
Howard, C.J. et al. (1999) Dendritic cells in cattle: phenotype and function.
Vet Immunol Immunopathol. 72 (1-2): 119-24.
- Synonyms
- SIRP ALPHA
- UniProt
- O46631
- Entrez Gene
- SIRPA
- GO Terms
- GO:0016021 integral to membrane
- GO:0017124 SH3 domain binding
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