CD28 antibody | AV7

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Mouse anti Chicken CD28

Product Type
Monoclonal Antibody
Clone
AV7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5760 F IP 0.25 mg
Mouse anti Chicken CD28, clone AV7 recognizes the chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994).

Mouse anti chicken CD28, clone AV7 has been demonstrated to recognise Turkey CD28 (Powell et al. 2009).

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Chicken Leucocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD28 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for CD28 antibody

  1. Powell F et al. (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28.
    Dev Comp Immunol. 33 (4): 540-6.
  2. Young, J.R. et al. (1994) Monomeric homologue of mammalian CD28 is expressed on chicken T cells.
    J Immunol. 152 (8): 3848-51.