CD28 antibody | AV7

Mouse anti Chicken CD28

Product Type
Monoclonal Antibody
Clone
AV7
Isotype
IgG1
Specificity
CD28

Product Code Applications Pack Size List Price Your Price Qty
MCA5760
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.25 mg
List Price Your Price
Search for Batch Specific Datasheets

Mouse anti Chicken CD28, clone AV7 recognizes the chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994).

Mouse anti chicken CD28, clone AV7 has been demonstrated to recognise Turkey CD28 (Powell et al. 2009).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Chicken Leucocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for CD28 antibody

  1. Powell F et al. (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28.
    Dev Comp Immunol. 33 (4): 540-6.
  2. Young, J.R. et al. (1994) Monomeric homologue of mammalian CD28 is expressed on chicken T cells.
    J Immunol. 152 (8): 3848-51.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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