CD14 antibody | AV141
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E ResetMouse anti Chicken CD14
- Product Type
- Monoclonal Antibody
- Clone
- AV141
- Isotype
- IgG1
- Specificity
- CD14
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD14 antibody, clone AV141 recognises chicken monocyte differentiation antigen CD14, a 465 amino acid GPI-anchored membrane glycoprotein containing multiple http://www.ebi.ac.uk/interpro/entry/IPR026906
. CD14 is a monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Clone AV141 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to Mouse anti Chicken CD14 antibody, clone AV141, other antibodies to chicken CD antigens and chicken cytokines from this project are now available from Bio-Rad. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken CD14/huIg fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product is suitable for use in indirect ELISA applications.
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| Description | Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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- UniProt
- B0BL87
MCA5926GA
150082
156889
163251
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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