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Bu-1a/b antibody | AV20

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Anti Chicken Bu-1a/b Antibody, clone AV20 thumbnail image 1 Anti Chicken Bu-1a/b Antibody, clone AV20 thumbnail image 2 Anti Chicken Bu-1a/b Antibody, clone AV20 thumbnail image 3 Anti Chicken Bu-1a/b Antibody, clone AV20 thumbnail image 4 Anti Chicken Bu-1a/b Antibody, clone AV20 thumbnail image 5
Anti Chicken Bu-1a/b Antibody, clone AV20 gallery image 1
Published customer image:
Mouse anti ChickenBu-1a/b antibody, clone AV20 (MCA5764) used to identify Bu-1 expressing cells in chicken conjunctiva associated lymphoid tissue by immunofluorescence.
Image caption:
Cellular infiltrates of head-associated lymphoid tissues following vaccination with BeauR-M41(S1) and BeauR-QX(S1). Cryosections were stained with monoclonal antibodies to detect Bu-1+ (green) and CD8α+ (red) cells. Nuclei were labeled with DAPI (blue). Scale bars, 50 μm. Representative images are shown in all panels.

From: Ellis S, Keep S, Britton P, de Wit S, Bickerton E, Vervelde L.
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol. 2018 Nov 12;92(23):e01473-18.
doi: 10.1128/JVI.01473-18.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken Bu-1a/b Antibody, clone AV20 gallery image 2
Published customer image:
Mouse anti Chicken Bu1a/b antibody, clone AV20 (MCA5764) used to evaluate chB6 expression on chicken lung cells by flow cytometry.
Image caption:
Phenotyping of lung cells infected with APEC by flow cytometry. Gradient purified lung cells were isolated and subjected to flow cytometric analysis to phenotype APECpos cells against a panel of cell surface markers. (C) Representative graphs of APEC O2-GFP inoculated birds gated for single live cells and plotted identically to the APEC O1-GFP inoculated birds without the GRL1 and GRL2 antibody staining which was only performed with APEC O1-GFP inoculated birds. APEC O2-GFPpos cells were CD45pos CD11pos, and CD3neg chB6neg K1. Again, a positive and negative MRC1L-B and MHC II cell population was detected within the APEC O2-GFPpos cells.

From: Alber A, Morris KM, Bryson KJ, Sutton KM, Monson MS, Chintoan-Uta C, Borowska D, Lamont SJ, Schouler C, Kaiser P, Stevens MP, Vervelde L.
Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
Front Immunol. 2020 Jan 10;10:3055.
doi: 10.3389/fimmu.2019.03055.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken Bu-1a/b Antibody, clone AV20 gallery image 3
Published customer image:
Mouse anti Chicken Bu1a/b antibody, clone AV20 (MCA5764) used to evaluate chB6 expression on chicken lung cells by flow cytometry.
Image caption:
Phenotyping of lung cells infected with APEC by flow cytometry. Gradient purified lung cells were isolated and subjected to flow cytometric analysis to phenotype APECpos cells against a panel of cell surface markers. (A) Representative graphs of APEC O1-GFP inoculated birds gated for single, live cells. APEC O1-GFPpos cells were gated against CSF1R-transgene in the top left graph visualising the APEC O1-GFPpos cells amongst the CSF1R-tgneg, CSF1R-tglow and CSF1R-tghigh cells. The horizontal gate divides the CSF1R-tgneg and CSF1R-tglow cells. Further phenotypic analysis showed that APEC O1-GFPpos cells are CD45pos CD11pos GRL1pos GRL2pos, and CD3neg chB6neg K1neg/low. A positive and negative MRC1L-B and MHC II cell population was detected within the APEC O1-GFPpos cells.

From: Alber A, Morris KM, Bryson KJ, Sutton KM, Monson MS, Chintoan-Uta C, Borowska D, Lamont SJ, Schouler C, Kaiser P, Stevens MP, Vervelde L.
Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
Front Immunol. 2020 Jan 10;10:3055.
doi: 10.3389/fimmu.2019.03055.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken Bu-1a/b Antibody, clone AV20 gallery image 4
Published customer image:
Mouse anti Chicken Bu-1a/b antibody, clone AV20 (MCA5764) used to label chicken non-B cells by flow cytometry.
Image caption:
chB6 expression on non-B cells: an IEL preparation was stained with antibodies against chB6 (AV20), TCRγδ(TCR1), and BAFF-R (2C4). Cells were gated for viable, single leukocytes. Gating on chB6+ cells (A) reveals that chB6+ cells consist of three subsets: BAFF-R+/TCRγδ− B cells, BAFF-R−/TCRγδ+ γδ T cells, and so far, uncharacterised BAFF-R−/TCRγδ− cells (B).

From: Citation: Härtle S, Sutton K, Vervelde L and Dalgaard TS (2024)
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry.
Front. Vet. Sci. 11:1385400.
doi: 10.3389/fvets.2024.1385400
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken Bu-1a/b Antibody, clone AV20 gallery image 5
Published customer image:
Mouse anti Chicken Bu-1a/b antibody, clone AV20 (MCA5764) used to identify chicken B cells by flow cytometry following conjugation to allophycocyanin.
Image caption:
(b) Flow cytometry gating strategy used for the analysis of splenocytes that underwent antibody staining for the identification of avian lymphocytes (CD4+, CD8α+, γδTCR+, CD4+γδTCR+) and antigen-presenting avian phagocytes (MRCL1-B+, MHC-II+, MRCL1-B+MHC-II+hi, MRCL1-B+MHC-II+lo). FSC-Area/FSC-Linear doublet discrimination (singlets) ensured that mostly single cells were selected for analysis. This was flowed by exclusion of dead cells using the fluorescent intercalator 7AAD, that undergoes a spectral shift upon association with DNA. Finally, forward versus side scatter (FSC vs SSC) gating is commonly used to identify cells of interest based on size and granularity (complexity). Isotype controls are also represented in the overall strategy.

From:Saint-Martin V, Guillory V, Chollot M, Fleurot I, Kut E, Roesch F, Caballero I, Helloin E, Chambellon E, Ferguson B, Velge P, Kempf F, Trapp S, Guabiraba R.
The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
Commun Biol. 2024 Sep 20;7(1):1185.
doi: 10.1038/s42003-024-06815-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Chicken Bu-1a/b

Product Type
Monoclonal Antibody
Clone
AV20
Isotype
IgG1
Specificity
Bu-1a/b

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Product Code Applications Pack Size List Price Your Price Qty
MCA5764
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader

Mouse anti Chicken Bu-1a/b antibody, clone AV20 recognizes Chicken Bu-1a/b, also known as chB6, a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that recognises Bu-1a but not Bu-1b is available.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Guinea Fowl
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Bursal cells from day old H.B15 (Bu-1a/b) chickens.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Source Reference

  1. Rothwell, C.J. et al. (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20.
    Vet Immunol Immunopathol. 55 (1-3): 225-34.

References for Bu-1a/b antibody

  1. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
    Immunogenetics. 44 (3): 212-7.
  2. Baigent, S.J. & Davison, T.F. (1999) Development and composition of lymphoid lesions in the spleens of Marek's disease virus-infected chickens: Association with virus spread and the pathogenesis of Marek's disease.
    Avian Pathol. 28 (3): 287-300.
  3. Barrow, A.D. et al. (2003) Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus.
    J Gen Virol. 84 (Pt 10): 2635-45.
  4. Tregaskes, C.A. et al. (2005) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
    Dev Comp Immunol. 29 (4): 361-74.
  5. Igyár;rtó BZ et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87 (2): 351-5.
  6. Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
    Poult Sci. 91 (2): 383-92.
  7. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  8. Sadeyen, J.R. et al. (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli. in chickens.
    Vaccine. 33 (31): 3624-7.
    9. View The Latest Product References
  9. Schermuly, J. et al. (2015) In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
    Proc Natl Acad Sci U S A. 112 (23): 7279-84.
  10. Ellis, S. et al. (2018) Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
    J Virol.92 (23): e01473-18.
  11. Alber, A. et al. (2019) Avian Pathogenic Escherichia coli (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in CSF1R-Reporter Transgenic Chickens.
    Front Immunol. 10: 3055.
  12. Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
    Frontiers in Veterinary Science. 23 May [Epub ahead of print].
  13. Saint-Martin, V. et al. (2024) The gut microbiota and its metabolite butyrate shape metabolism and antiviral immunity along the gut-lung axis in the chicken.
    Commun Biol. 7 (1): 1185.

Synonyms
Bursal Antigen 1 A/B
RRID
AB_10720221
UniProt
Q90747
Q90746

MCA5764

150541 161337 167960

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