Mouse anti ChickenBu-1a/b antibody, clone AV20
recognizes Chicken Bu-1a/b, also known as chB6, a type 1 transmembrane protein.
Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22
that recognises Bu-1a but not Bu-1b is available.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Bursal cells from day old H.B15 (Bu-1a/b) chickens.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Bu-1a/b antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for Bu-1a/b antibody
Igyár;rtó BZ et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
Poult Sci. 87 (2): 351-5.
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65.
Schermuly, J. et al. (2015) In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
Proc Natl Acad Sci U S A. 112 (23): 7279-84.
Tregaskes, C.A. et al. (2005) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
Dev Comp Immunol. 29 (4): 361-74.
Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
Immunogenetics. 44 (3): 212-7.
Baigent, S. J. & Davison, T. F. (1999) Development and composition of lymphoid lesions in the spleens of Marek's disease virus-infected chickens: Association with virus spread and the pathogenesis of Marek's disease
Avian Pathology. 28 (3): 287-300.
Barrow, A.D. et al. (2003) Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus.
J Gen Virol. 84 (Pt 10): 2635-45.
Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
Poult Sci. 91 (2): 383-92.
Rothwell, C.J. et al. (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20.
Vet Immunol Immunopathol. 55 (1-3): 225-34.
Sadeyen, J.R. et al. (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli in chickens.
Vaccine. 33 (31): 3624-7.