Bu-1a/b antibody | AV20
Mouse anti ChickenBu-1a/b antibody, clone AV20 (MCA5764) used to identify Bu-1 expressing cells in chicken conjunctiva associated lymphoid tissue by immunofluorescence.
Image caption:
Cellular infiltrates of head-associated lymphoid tissues following vaccination with BeauR-M41(S1) and BeauR-QX(S1). Cryosections were stained with monoclonal antibodies to detect Bu-1+ (green) and CD8α+ (red) cells. Nuclei were labeled with DAPI (blue). Scale bars, 50 μm. Representative images are shown in all panels.
From: Ellis S, Keep S, Britton P, de Wit S, Bickerton E, Vervelde L.
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol. 2018 Nov 12;92(23):e01473-18.
doi: 10.1128/JVI.01473-18.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF ResetMouse anti Chicken Bu-1a/b
- Product Type
- Monoclonal Antibody
- Clone
- AV20
- Isotype
- IgG1
- Specificity
- Bu-1a/b
Antibody Quality and Performance Guarantee
| Mouse anti ChickenBu-1a/b antibody, clone AV20 recognizes Chicken Bu-1a/b, also known as chB6, a type 1 transmembrane protein. Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that recognises Bu-1a but not Bu-1b is available. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Quail Turkey Guinea Fowl - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Bursal cells from day old H.B15 (Bu-1a/b) chickens.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Source Reference
-
Veromaa, T. et al. (1988) Monoclonal Antibodies Against Chicken Bu-la and Bu-lb Alloantigens
Hybridoma 7 (1): 41-8.
References for Bu-1a/b antibody
-
Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
Immunogenetics. 44 (3): 212-7. -
Rothwell, C.J. et al. (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20.
Vet Immunol Immunopathol. 55 (1-3): 225-34. -
Baigent, S. J. & Davison, T. F. (1999) Development and composition of lymphoid lesions in the spleens of Marek's disease virus-infected chickens: Association with virus spread and the pathogenesis of Marek's disease
Avian Pathology. 28 (3): 287-300. -
Barrow, A.D. et al. (2003) Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus.
J Gen Virol. 84 (Pt 10): 2635-45. -
Tregaskes, C.A. et al. (2005) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
Dev Comp Immunol. 29 (4): 361-74. -
Igyár;rtó BZ et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
Poult Sci. 87 (2): 351-5. -
Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
Poult Sci. 91 (2): 383-92. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65.
View The Latest Product References
-
Sadeyen, J.R. et al. (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli. in chickens.
Vaccine. 33 (31): 3624-7. -
Schermuly, J. et al. (2015) In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
Proc Natl Acad Sci U S A. 112 (23): 7279-84. -
Ellis, S. et al. (2018) Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol.92 (23): e01473-18.
- Synonyms
- Bursal Antigen 1 A/B
- RRID
- AB_10720221
- UniProt
- Q90747
- Q90746
MCA5764
150541
161337
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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