Bu-1a/b antibody | AV20

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Mouse anti Chicken Bu-1a/b

Product Type
Monoclonal Antibody
Clone
AV20
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5764 C F IP 0.25 mg
Mouse anti ChickenBu-1a/b antibody, clone AV20 recognizes Chicken Bu-1a/b, also known as chB6, a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that recognises Bu-1a but not Bu-1b is available.

Product Details

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Quail
Turkey
Guinea Fowl
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Bursal cells from day old H.B15 (Bu-1a/b) chickens.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q90747 Related reagents
Q90746 Related reagents
Regulatory
For research purposes only

Applications of Bu-1a/b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Bu-1a/b antibody

  1. Igyár;rtó BZ et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87 (2): 351-5.
  2. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  3. Schermuly, J. et al. (2015) In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
    Proc Natl Acad Sci U S A. 112 (23): 7279-84.
  4. Tregaskes, C.A. et al. (2005) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
    Dev Comp Immunol. 29 (4): 361-74.
  5. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
    Immunogenetics. 44 (3): 212-7.
  6. Baigent, S. J. & Davison, T. F. (1999) Development and composition of lymphoid lesions in the spleens of Marek's disease virus-infected chickens: Association with virus spread and the pathogenesis of Marek's disease
    Avian Pathology. 28 (3): 287-300.
  7. Barrow, A.D. et al. (2003) Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus.
    J Gen Virol. 84 (Pt 10): 2635-45.
  8. Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
    Poult Sci. 91 (2): 383-92.
  9. Rothwell, C.J. et al. (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20.
    Vet Immunol Immunopathol. 55 (1-3): 225-34.
  10. Sadeyen, J.R. et al. (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli in chickens.
    Vaccine. 33 (31): 3624-7.