Bu-1a/b antibody | AV20

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Mouse anti Chicken Bu-1a/b

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.25 mg loader

Mouse anti ChickenBu-1a/b antibody, clone AV20 recognizes Chicken Bu-1a/b, also known as chB6, a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that recognises Bu-1a but not Bu-1b is available.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Guinea Fowl
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Bursal cells from day old H.B15 (Bu-1a/b) chickens.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

For research purposes only

Applications of Bu-1a/b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Bu-1a/b antibody

  1. Tregaskes, C.A. et al. (1996) Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure.
    Immunogenetics. 44 (3): 212-7.
  2. Rothwell, C.J. et al. (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20.
    Vet Immunol Immunopathol. 55 (1-3): 225-34.
  3. Baigent, S. J. & Davison, T. F. (1999) Development and composition of lymphoid lesions in the spleens of Marek's disease virus-infected chickens: Association with virus spread and the pathogenesis of Marek's disease
    Avian Pathology. 28 (3): 287-300.
  4. Barrow, A.D. et al. (2003) Infection of macrophages by a lymphotropic herpesvirus: a new tropism for Marek's disease virus.
    J Gen Virol. 84 (Pt 10): 2635-45.
  5. Tregaskes, C.A. et al. (2005) Conservation of biological properties of the CD40 ligand, CD154 in a non-mammalian vertebrate.
    Dev Comp Immunol. 29 (4): 361-74.
  6. Igyár;rtó BZ et al. (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody.
    Poult Sci. 87 (2): 351-5.
  7. Meyerhoff, R.R. et al. (2012) Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys.
    Poult Sci. 91 (2): 383-92.
  8. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  9. Sadeyen, J.R. et al. (2015) A cyclophosphamide-sensitive cell compartment is essential for homologous protection conferred by licensed vaccines for the control of avian pathogenic Escherichia coli. in chickens.
    Vaccine. 33 (31): 3624-7.
  10. Schermuly, J. et al. (2015) In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
    Proc Natl Acad Sci U S A. 112 (23): 7279-84.

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