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Mouse anti Chicken Bu-1 antibody, clone L22 used for immunofluorescence studies.
Confocal analysis of MacRed chicken post-hatch mononuclear phagocyte populations. (A) Splenic mononuclear phagocytes (red) and Bu-1+ B-cells (green) from a 16-week-old MacRed chicken. Rings of transgene-expressing cells can clearly be seen surrounding the ellipsoid (asterisk). (B) Bursa of Fabricius from an 8-day-old MacRed chicken: Bu-1+ B cells (green) show arrangement of the B-cell follicles; mononuclear phagocytes (red) are present in the medulla (M) and interfollicular region (red arrow), but not in the cortex (C) of B-cell follicles in the bursa of Fabricius. (C) Caecal tonsil B-cell follicle from a 10-week-old MacRed chicken, showing location of mononuclear phagocytes (red) and Bu-1+ B-cells (green). Transgene-expressing cells concentrated in the medulla region (M) of the B-cell follicle are a dense network of FDC. (D) Microglial cells (red) in the cerebellum of an 8-day-old MacRed chicken showing colocalisation with CD45 staining (green). (E) Kupffer cells (red) showing colocalisation with CSF1R (green) from a 13-week-old MacRed chicken liver. (F) Lung mononuclear phagocytes (red) and Bu-1+ B-cells (green) in the interstitial tissue of the parabronchial wall from a 16-week-old MacRed chicken. The parabronchial lumen (pb) is indicated. (G) Epidermal mononuclear phagocyte cells (red) in epidermal sheet preparation from a 10-week-old MacRed chicken. (H) Breast muscle mononuclear phagocytes (red) from a 16-week-old MacRed chicken co-expressing MHCII (green). (I) Feather pulp mononuclear phagocytes from an 8-day-old MacRed chicken (red) co-stained with CD45 (green). Scale bars: 50 μm.
From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.